Enantioseparation of the constituents involved in the phenylalanine-tyrosine metabolic pathway by capillary electrophoresis tandem mass spectrometry

Catecholamines dopamine, norepinephrine, and epinephrine are well-known neurotransmitters playing different roles in the nervous and endocrine system. These compounds are biologically synthesized in the phenylalanine-tyrosine pathway which consists on the successive conversion of L-phenylalanine int...

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Autores: Sánchez López, Elena, Marcos González, Alberto, Ambrosio, Emilio, Marina Alegre, María Luisa|||0000-0002-5583-1624, Crego Navazo, Antonio Luis|||0000-0002-4083-5294
Tipo de recurso: artículo
Fecha de publicación:2016
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/48166
Acceso en línea:http://hdl.handle.net/10017/48166
https://dx.doi.org/10.1016/j.Chroma.2016.06.053
Access Level:acceso abierto
Palabra clave:Capillary electrophoresis
Enantiomeric multicomponent separation
Phenylalanine-tyrosine metabolic pathway
Plasma sample
Tandem mass spectrometry
Química
Chemistry
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spelling Enantioseparation of the constituents involved in the phenylalanine-tyrosine metabolic pathway by capillary electrophoresis tandem mass spectrometrySánchez López, ElenaMarcos González, AlbertoAmbrosio, EmilioMarina Alegre, María Luisa|||0000-0002-5583-1624Crego Navazo, Antonio Luis|||0000-0002-4083-5294Capillary electrophoresisEnantiomeric multicomponent separationPhenylalanine-tyrosine metabolic pathwayPlasma sampleTandem mass spectrometryQuímicaChemistryCatecholamines dopamine, norepinephrine, and epinephrine are well-known neurotransmitters playing different roles in the nervous and endocrine system. These compounds are biologically synthesized in the phenylalanine-tyrosine pathway which consists on the successive conversion of L-phenylalanine into L-tyrosine, L-3,4-dihydroxyphenylalanine (L-DOPA), dopamine, norepinephrine, and epinephrine. This work describes the development of an enantioselective CE-ESI-MS2 methodology enabling, for the first time, the simultaneous enantioseparation of all the constituents involved in the Phe-Tyr metabolic pathway, since all these compounds except dopamine are chiral. The developed method was based on the use of a dual CDs system formed by 180 mM of methyl-beta-CD and 40 mM of 2-hydroxypropy1-beta-CD dissolved in 2 M formic acid (pH 1.2) and presented the advantage of avoiding the use of any time-consuming labelling procedure. LODs ranged from 40 to 150 nM and the unequivocal identification of the compounds investigated was achieved through their MS2 spectra. The applicability of this methodology to the analysis of biological samples (rat plasma) was also demonstrated. (C) 2016 Elsevier B.V. All rights reserved.Ministerio de Economía y Competitividad20162016-10-07journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/48166https://dx.doi.org/10.1016/j.Chroma.2016.06.053reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available CTQ2013-48740-P Metodolgoías avanzadas para análisis quiral mediante técnicas microseparativas. Búsqueda de biomarcadores en sistemas enantioselectivosopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-ShareAlike 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-sa/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/481662026-06-18T11:13:07Z
dc.title.none.fl_str_mv Enantioseparation of the constituents involved in the phenylalanine-tyrosine metabolic pathway by capillary electrophoresis tandem mass spectrometry
title Enantioseparation of the constituents involved in the phenylalanine-tyrosine metabolic pathway by capillary electrophoresis tandem mass spectrometry
spellingShingle Enantioseparation of the constituents involved in the phenylalanine-tyrosine metabolic pathway by capillary electrophoresis tandem mass spectrometry
Sánchez López, Elena
Capillary electrophoresis
Enantiomeric multicomponent separation
Phenylalanine-tyrosine metabolic pathway
Plasma sample
Tandem mass spectrometry
Química
Chemistry
title_short Enantioseparation of the constituents involved in the phenylalanine-tyrosine metabolic pathway by capillary electrophoresis tandem mass spectrometry
title_full Enantioseparation of the constituents involved in the phenylalanine-tyrosine metabolic pathway by capillary electrophoresis tandem mass spectrometry
title_fullStr Enantioseparation of the constituents involved in the phenylalanine-tyrosine metabolic pathway by capillary electrophoresis tandem mass spectrometry
title_full_unstemmed Enantioseparation of the constituents involved in the phenylalanine-tyrosine metabolic pathway by capillary electrophoresis tandem mass spectrometry
title_sort Enantioseparation of the constituents involved in the phenylalanine-tyrosine metabolic pathway by capillary electrophoresis tandem mass spectrometry
dc.creator.none.fl_str_mv Sánchez López, Elena
Marcos González, Alberto
Ambrosio, Emilio
Marina Alegre, María Luisa|||0000-0002-5583-1624
Crego Navazo, Antonio Luis|||0000-0002-4083-5294
author Sánchez López, Elena
author_facet Sánchez López, Elena
Marcos González, Alberto
Ambrosio, Emilio
Marina Alegre, María Luisa|||0000-0002-5583-1624
Crego Navazo, Antonio Luis|||0000-0002-4083-5294
author_role author
author2 Marcos González, Alberto
Ambrosio, Emilio
Marina Alegre, María Luisa|||0000-0002-5583-1624
Crego Navazo, Antonio Luis|||0000-0002-4083-5294
author2_role author
author
author
author
dc.subject.none.fl_str_mv Capillary electrophoresis
Enantiomeric multicomponent separation
Phenylalanine-tyrosine metabolic pathway
Plasma sample
Tandem mass spectrometry
Química
Chemistry
topic Capillary electrophoresis
Enantiomeric multicomponent separation
Phenylalanine-tyrosine metabolic pathway
Plasma sample
Tandem mass spectrometry
Química
Chemistry
description Catecholamines dopamine, norepinephrine, and epinephrine are well-known neurotransmitters playing different roles in the nervous and endocrine system. These compounds are biologically synthesized in the phenylalanine-tyrosine pathway which consists on the successive conversion of L-phenylalanine into L-tyrosine, L-3,4-dihydroxyphenylalanine (L-DOPA), dopamine, norepinephrine, and epinephrine. This work describes the development of an enantioselective CE-ESI-MS2 methodology enabling, for the first time, the simultaneous enantioseparation of all the constituents involved in the Phe-Tyr metabolic pathway, since all these compounds except dopamine are chiral. The developed method was based on the use of a dual CDs system formed by 180 mM of methyl-beta-CD and 40 mM of 2-hydroxypropy1-beta-CD dissolved in 2 M formic acid (pH 1.2) and presented the advantage of avoiding the use of any time-consuming labelling procedure. LODs ranged from 40 to 150 nM and the unequivocal identification of the compounds investigated was achieved through their MS2 spectra. The applicability of this methodology to the analysis of biological samples (rat plasma) was also demonstrated. (C) 2016 Elsevier B.V. All rights reserved.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-10-07
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/48166
https://dx.doi.org/10.1016/j.Chroma.2016.06.053
url http://hdl.handle.net/10017/48166
https://dx.doi.org/10.1016/j.Chroma.2016.06.053
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available CTQ2013-48740-P Metodolgoías avanzadas para análisis quiral mediante técnicas microseparativas. Búsqueda de biomarcadores en sistemas enantioselectivos
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-ShareAlike 4.0 International
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-ShareAlike 4.0 International
http://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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