Iflavirus covert infection increases susceptibility to nucleopolyhedrovirus disease in Spodoptera exigua

Naturally occurring covert infections in lepidopteran populations can involve multiple viruses with potentially different transmission strategies. In this study, we characterized covert infection by two RNA viruses, Spodoptera exigua iflavirus 1 (SeIV-1) and Spodoptera exigua iflavirus 2 (SeIV-2) (f...

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Autores: Carballo Palos, Arkaitz, Williams, Trevor, Murillo Pérez, Rosa, Caballero Murillo, Primitivo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/37341
Acceso en línea:https://hdl.handle.net/2454/37341
Access Level:acceso abierto
Palabra clave:Iflavirus
SeMNPV
Covert infection
Spodoptera exigua
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spelling Iflavirus covert infection increases susceptibility to nucleopolyhedrovirus disease in Spodoptera exiguaCarballo Palos, ArkaitzWilliams, TrevorMurillo Pérez, RosaCaballero Murillo, PrimitivoIflavirusSeMNPVCovert infectionSpodoptera exiguaNaturally occurring covert infections in lepidopteran populations can involve multiple viruses with potentially different transmission strategies. In this study, we characterized covert infection by two RNA viruses, Spodoptera exigua iflavirus 1 (SeIV-1) and Spodoptera exigua iflavirus 2 (SeIV-2) (family Iflaviridae) that naturally infect populations of Spodoptera exigua, and examined their influence on susceptibility to patent disease by the nucleopolyhedrovirus Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) (family Baculoviridae). The abundance of SeIV-1 genomes increased up to ten-thousand-fold across insect developmental stages after surface contamination of host eggs with a mixture of SeIV-1 and SeIV-2 particles, whereas the abundance of SeIV-2 remained constant across all developmental stages. Low levels of SeIV-2 infection were detected in all groups of insects, including those that hatched from surface-decontaminated egg masses. SeIV-1 infection resulted in reduced larval weight gain, and an unbalanced sex ratio, whereas larval developmental time, pupal weight, and adult emergence and fecundity were not significantly affected in infected adults. The inoculation of S. exigua egg masses with iflavirus, followed by a subsequent infection with SeMNPV, resulted in an additive effect on larval mortality. The 50% lethal concentration (LC50) of SeMNPV was reduced nearly 4-fold and the mean time to death was faster by 12 h in iflavirus-treated insects. These results suggest that inapparent iflavirus infections may be able to modulate the host response to a new pathogen, a finding that has particular relevance to the use of SeMNPV as the basis for biological pest control products.This research was funded by Spanish Ministry of Science and Innovation (AGL2011-30352-C02-01). AC received a doctoral grant from AGL2011-30352-C02-01.MDPIAgronomia, Bioteknologia eta ElikaduraInstitute for Multidisciplinary Research in Applied Biology - IMABAgronomía, Biotecnología y Alimentación2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/ziphttps://hdl.handle.net/2454/37341reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/MICINN//AGL2011-30352-C02-01© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/373412026-06-17T12:41:47Z
dc.title.none.fl_str_mv Iflavirus covert infection increases susceptibility to nucleopolyhedrovirus disease in Spodoptera exigua
title Iflavirus covert infection increases susceptibility to nucleopolyhedrovirus disease in Spodoptera exigua
spellingShingle Iflavirus covert infection increases susceptibility to nucleopolyhedrovirus disease in Spodoptera exigua
Carballo Palos, Arkaitz
Iflavirus
SeMNPV
Covert infection
Spodoptera exigua
title_short Iflavirus covert infection increases susceptibility to nucleopolyhedrovirus disease in Spodoptera exigua
title_full Iflavirus covert infection increases susceptibility to nucleopolyhedrovirus disease in Spodoptera exigua
title_fullStr Iflavirus covert infection increases susceptibility to nucleopolyhedrovirus disease in Spodoptera exigua
title_full_unstemmed Iflavirus covert infection increases susceptibility to nucleopolyhedrovirus disease in Spodoptera exigua
title_sort Iflavirus covert infection increases susceptibility to nucleopolyhedrovirus disease in Spodoptera exigua
dc.creator.none.fl_str_mv Carballo Palos, Arkaitz
Williams, Trevor
Murillo Pérez, Rosa
Caballero Murillo, Primitivo
author Carballo Palos, Arkaitz
author_facet Carballo Palos, Arkaitz
Williams, Trevor
Murillo Pérez, Rosa
Caballero Murillo, Primitivo
author_role author
author2 Williams, Trevor
Murillo Pérez, Rosa
Caballero Murillo, Primitivo
author2_role author
author
author
dc.contributor.none.fl_str_mv Agronomia, Bioteknologia eta Elikadura
Institute for Multidisciplinary Research in Applied Biology - IMAB
Agronomía, Biotecnología y Alimentación
dc.subject.none.fl_str_mv Iflavirus
SeMNPV
Covert infection
Spodoptera exigua
topic Iflavirus
SeMNPV
Covert infection
Spodoptera exigua
description Naturally occurring covert infections in lepidopteran populations can involve multiple viruses with potentially different transmission strategies. In this study, we characterized covert infection by two RNA viruses, Spodoptera exigua iflavirus 1 (SeIV-1) and Spodoptera exigua iflavirus 2 (SeIV-2) (family Iflaviridae) that naturally infect populations of Spodoptera exigua, and examined their influence on susceptibility to patent disease by the nucleopolyhedrovirus Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) (family Baculoviridae). The abundance of SeIV-1 genomes increased up to ten-thousand-fold across insect developmental stages after surface contamination of host eggs with a mixture of SeIV-1 and SeIV-2 particles, whereas the abundance of SeIV-2 remained constant across all developmental stages. Low levels of SeIV-2 infection were detected in all groups of insects, including those that hatched from surface-decontaminated egg masses. SeIV-1 infection resulted in reduced larval weight gain, and an unbalanced sex ratio, whereas larval developmental time, pupal weight, and adult emergence and fecundity were not significantly affected in infected adults. The inoculation of S. exigua egg masses with iflavirus, followed by a subsequent infection with SeMNPV, resulted in an additive effect on larval mortality. The 50% lethal concentration (LC50) of SeMNPV was reduced nearly 4-fold and the mean time to death was faster by 12 h in iflavirus-treated insects. These results suggest that inapparent iflavirus infections may be able to modulate the host response to a new pathogen, a finding that has particular relevance to the use of SeMNPV as the basis for biological pest control products.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/37341
url https://hdl.handle.net/2454/37341
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MICINN//AGL2011-30352-C02-01
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/zip
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
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instname_str Universidad Pública de Navarra
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collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
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