Microsatellite typing of Lachancea thermotolerans for wine fermentation monitoring

Climate change is causing a lack of acidity during winemaking and oenologists use several solutions to cope with such a problem. Lachancea thermotolerans, which has the potential to tolerate the harsh physicochemical conditions of wine, has emerged as a promising alternative for pH management during...

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Detalhes bibliográficos
Autores: Vicente, Javier, Navascués, Eva, Benito, Santiago, Marquina Díaz, Domingo, Santos de la Sen, Antonio
Tipo de documento: artigo
Data de publicação:2023
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositório:Docta Complutense
Idioma:inglês
OAI Identifier:oai:docta.ucm.es:20.500.14352/88515
Acesso em linha:https://hdl.handle.net/20.500.14352/88515
Access Level:Acceso aberto
Palavra-chave:579
663.2
Climate change
Wine
Acidity
Lachancea thermotolerans
Genotyping
Fermentation
Microbiología (Biología)
2302.12 Fermentación
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spelling Microsatellite typing of Lachancea thermotolerans for wine fermentation monitoringVicente, JavierNavascués, EvaBenito, SantiagoMarquina Díaz, DomingoSantos de la Sen, Antonio579663.2Climate changeWineAcidityLachancea thermotoleransGenotypingFermentationMicrobiología (Biología)2302.12 FermentaciónClimate change is causing a lack of acidity during winemaking and oenologists use several solutions to cope with such a problem. Lachancea thermotolerans, which has the potential to tolerate the harsh physicochemical conditions of wine, has emerged as a promising alternative for pH management during winemaking and, currently, it is the most valuable yeast used for acidity control in wine. In this work a manageable method for L. thermotolerans genotyping based on a multiplexed microsatellite amplification in 6 different loci was developed. The proposed method was used to distinguish between 103 collection strains obtained from different geographical and isolation sources, and then challenged against a 429 L. thermotolerans isolates from several wineries and harvests. The procedure was also tested for fermentation monitoring and strain implantation. This approach was conceived to simplify the methodology available for L. thermotolerans genotyping, making it easy for applying in wine-related laboratories. This method can be applied to distinguish between L. thermotolerans strains in selection programs and to follow implantation of inoculated strains during winemaking with optimal results.ElsevierUniversidad Complutense de Madrid20232023-01-0120232023-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/88515reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/885152026-06-02T12:44:21Z
dc.title.none.fl_str_mv Microsatellite typing of Lachancea thermotolerans for wine fermentation monitoring
title Microsatellite typing of Lachancea thermotolerans for wine fermentation monitoring
spellingShingle Microsatellite typing of Lachancea thermotolerans for wine fermentation monitoring
Vicente, Javier
579
663.2
Climate change
Wine
Acidity
Lachancea thermotolerans
Genotyping
Fermentation
Microbiología (Biología)
2302.12 Fermentación
title_short Microsatellite typing of Lachancea thermotolerans for wine fermentation monitoring
title_full Microsatellite typing of Lachancea thermotolerans for wine fermentation monitoring
title_fullStr Microsatellite typing of Lachancea thermotolerans for wine fermentation monitoring
title_full_unstemmed Microsatellite typing of Lachancea thermotolerans for wine fermentation monitoring
title_sort Microsatellite typing of Lachancea thermotolerans for wine fermentation monitoring
dc.creator.none.fl_str_mv Vicente, Javier
Navascués, Eva
Benito, Santiago
Marquina Díaz, Domingo
Santos de la Sen, Antonio
author Vicente, Javier
author_facet Vicente, Javier
Navascués, Eva
Benito, Santiago
Marquina Díaz, Domingo
Santos de la Sen, Antonio
author_role author
author2 Navascués, Eva
Benito, Santiago
Marquina Díaz, Domingo
Santos de la Sen, Antonio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 579
663.2
Climate change
Wine
Acidity
Lachancea thermotolerans
Genotyping
Fermentation
Microbiología (Biología)
2302.12 Fermentación
topic 579
663.2
Climate change
Wine
Acidity
Lachancea thermotolerans
Genotyping
Fermentation
Microbiología (Biología)
2302.12 Fermentación
description Climate change is causing a lack of acidity during winemaking and oenologists use several solutions to cope with such a problem. Lachancea thermotolerans, which has the potential to tolerate the harsh physicochemical conditions of wine, has emerged as a promising alternative for pH management during winemaking and, currently, it is the most valuable yeast used for acidity control in wine. In this work a manageable method for L. thermotolerans genotyping based on a multiplexed microsatellite amplification in 6 different loci was developed. The proposed method was used to distinguish between 103 collection strains obtained from different geographical and isolation sources, and then challenged against a 429 L. thermotolerans isolates from several wineries and harvests. The procedure was also tested for fermentation monitoring and strain implantation. This approach was conceived to simplify the methodology available for L. thermotolerans genotyping, making it easy for applying in wine-related laboratories. This method can be applied to distinguish between L. thermotolerans strains in selection programs and to follow implantation of inoculated strains during winemaking with optimal results.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/88515
url https://hdl.handle.net/20.500.14352/88515
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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