Dynamic Interactions between Autophagosomes and Lipid Droplets in Chlamydomonas reinhardtii

Autophagy is a highly conserved catabolic process in eukaryotic cells by which waste cellular components are recycled to maintain growth in both favorable and stress conditions. Autophagy has been linked to lipid metabolism in microalgae; however, the mechanism underlying this interaction remains un...

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Autores: Tran, Quynh-Giao, Yoon, Hyang Ran, Cho, Kichul, Lee, Seon-Jin, Crespo, José L., Ramanan, Rishiram, Kim, Hee-Sik
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/191351
Acceso en línea:http://hdl.handle.net/10261/191351
Access Level:acceso abierto
Palabra clave:Autophagy marker
Chlamydomonas
Chloroquine
Lipid droplet
mCherry-ATG8
Microalgal lipophagy
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spelling Dynamic Interactions between Autophagosomes and Lipid Droplets in Chlamydomonas reinhardtiiTran, Quynh-GiaoYoon, Hyang RanCho, KichulLee, Seon-JinCrespo, José L.Ramanan, RishiramKim, Hee-SikAutophagy markerChlamydomonasChloroquineLipid dropletmCherry-ATG8Microalgal lipophagyAutophagy is a highly conserved catabolic process in eukaryotic cells by which waste cellular components are recycled to maintain growth in both favorable and stress conditions. Autophagy has been linked to lipid metabolism in microalgae; however, the mechanism underlying this interaction remains unclear. In this study, transgenic Chlamydomonas reinhardtii cells that stably express the red fluorescent protein (mCherry) tagged-ATG8 as an autophagy marker were established. By using this tool, we were able to follow the autophagy process in live microalgal cells under various conditions. Live-cell and transmission electron microscopy (TEM) imaging revealed physical contacts between lipid droplets and autophagic structures during the early stage of nitrogen starvation, while fusion of these two organelles was observed in prolonged nutritional deficiency, suggesting that an autophagy-related pathway might be involved in lipid droplet turnover in this alga. Our results thus shed light on the interplay between autophagy and lipid metabolism in C. reinhardtii, and this autophagy marker would be a valuable asset for further investigations on autophagic processes in microalgae.This research was funded by a grant from Marine Biotechnology Program (20150184) funded by Ministry of Oceans and Fisheries, Korea; by the Advanced Biomass R&D Center (ABC) of Global Frontier Project funded by the Ministry of Science and ICT(ABC-2015M3A6A2065697); and by grant from the Korea Research Institute of Bioscience and Biotechnology (KRIBB) Research Initiative Program (www.kribb.re.kr).Peer reviewedMultidisciplinary Digital Publishing InstituteMinistry of Oceans and Fisheries (South Korea)Korea Research Institute of Bioscience & BiotechnologyMinistry of Science, ICT and Future Planning (South Korea)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/191351reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.3390/cells8090992Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1913512026-05-22T06:33:51Z
dc.title.none.fl_str_mv Dynamic Interactions between Autophagosomes and Lipid Droplets in Chlamydomonas reinhardtii
title Dynamic Interactions between Autophagosomes and Lipid Droplets in Chlamydomonas reinhardtii
spellingShingle Dynamic Interactions between Autophagosomes and Lipid Droplets in Chlamydomonas reinhardtii
Tran, Quynh-Giao
Autophagy marker
Chlamydomonas
Chloroquine
Lipid droplet
mCherry-ATG8
Microalgal lipophagy
title_short Dynamic Interactions between Autophagosomes and Lipid Droplets in Chlamydomonas reinhardtii
title_full Dynamic Interactions between Autophagosomes and Lipid Droplets in Chlamydomonas reinhardtii
title_fullStr Dynamic Interactions between Autophagosomes and Lipid Droplets in Chlamydomonas reinhardtii
title_full_unstemmed Dynamic Interactions between Autophagosomes and Lipid Droplets in Chlamydomonas reinhardtii
title_sort Dynamic Interactions between Autophagosomes and Lipid Droplets in Chlamydomonas reinhardtii
dc.creator.none.fl_str_mv Tran, Quynh-Giao
Yoon, Hyang Ran
Cho, Kichul
Lee, Seon-Jin
Crespo, José L.
Ramanan, Rishiram
Kim, Hee-Sik
author Tran, Quynh-Giao
author_facet Tran, Quynh-Giao
Yoon, Hyang Ran
Cho, Kichul
Lee, Seon-Jin
Crespo, José L.
Ramanan, Rishiram
Kim, Hee-Sik
author_role author
author2 Yoon, Hyang Ran
Cho, Kichul
Lee, Seon-Jin
Crespo, José L.
Ramanan, Rishiram
Kim, Hee-Sik
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministry of Oceans and Fisheries (South Korea)
Korea Research Institute of Bioscience & Biotechnology
Ministry of Science, ICT and Future Planning (South Korea)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Autophagy marker
Chlamydomonas
Chloroquine
Lipid droplet
mCherry-ATG8
Microalgal lipophagy
topic Autophagy marker
Chlamydomonas
Chloroquine
Lipid droplet
mCherry-ATG8
Microalgal lipophagy
description Autophagy is a highly conserved catabolic process in eukaryotic cells by which waste cellular components are recycled to maintain growth in both favorable and stress conditions. Autophagy has been linked to lipid metabolism in microalgae; however, the mechanism underlying this interaction remains unclear. In this study, transgenic Chlamydomonas reinhardtii cells that stably express the red fluorescent protein (mCherry) tagged-ATG8 as an autophagy marker were established. By using this tool, we were able to follow the autophagy process in live microalgal cells under various conditions. Live-cell and transmission electron microscopy (TEM) imaging revealed physical contacts between lipid droplets and autophagic structures during the early stage of nitrogen starvation, while fusion of these two organelles was observed in prolonged nutritional deficiency, suggesting that an autophagy-related pathway might be involved in lipid droplet turnover in this alga. Our results thus shed light on the interplay between autophagy and lipid metabolism in C. reinhardtii, and this autophagy marker would be a valuable asset for further investigations on autophagic processes in microalgae.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/191351
url http://hdl.handle.net/10261/191351
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.3390/cells8090992
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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