A microdestructive capillary electrophoresis method for the analysis of blue-pen-ink strokes on office paper

This manuscript describes the development of a capillary electrophoresis (CE) method for the detection of acid and basic dyes and its application to real samples, blue-pen-ink strokes onoffice paper. First, a capillary zone electrophoresis (CZE) method was developed for the separation of basic and a...

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Detalles Bibliográficos
Autores: Calcerrada Guerreiro, Matías, García Ruiz, Carmen|||0000-0001-5925-3449, González Herráez, Miguel|||0000-0003-2555-2971
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/50108
Acceso en línea:http://hdl.handle.net/10017/50108
https://dx.doi.org/10.1016/j.chroma.2015.04.036
Access Level:acceso abierto
Palabra clave:Blue ink
blue pen
capillary electrophoresis
dyes
ink differentiation
profiling
Química
Chemistry
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spelling A microdestructive capillary electrophoresis method for the analysis of blue-pen-ink strokes on office paperCalcerrada Guerreiro, MatíasGarcía Ruiz, Carmen|||0000-0001-5925-3449González Herráez, Miguel|||0000-0003-2555-2971Blue inkblue pencapillary electrophoresisdyesink differentiationprofilingQuímicaChemistryThis manuscript describes the development of a capillary electrophoresis (CE) method for the detection of acid and basic dyes and its application to real samples, blue-pen-ink strokes onoffice paper. First, a capillary zone electrophoresis (CZE) method was developed for the separation of basic and acid dyes, by studying the separation medium (buffer nature, pH andrelative amount of additive) and instrumental parameters (temperature, voltage and capillary dimensions). The method performance was evaluated in terms of selectivity, resolution (above 5 and 2 for acid dyes and basic dyes, respectively, except for two basic dye standards), LOD (lower than 0.4 mg/L) and precision as intraday and interday RSD values of peak migrationtimes (lower than 0.6 %). The developed method was then applied to 34 blue pens from different technologies (rollerball, ballpoint, markers) and with different ink composition (gel, 30 water-based, oil-based). A microdestructive sample treatment using a scalpel to scratch 0.3 mg of ink stroke was performed. The entire electropherogram profile allowed the visual discrimination between different types of ink and brands, being not necessary a statistical treatment. A 100% of discrimination was achieved between pen technologies, brands, and models, although non-reproducible zones in the electropherograms were found for blue gel pen samples. The two different batches of blue oil-based pens were also differentiated. Thus, this method provides a simple, microdestructive, and rapid analysis of different blue pentechnologies which may complement the current analysis of questioned documents performed by forensic laboratories.20152015-06-26journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/50108https://dx.doi.org/10.1016/j.chroma.2015.04.036reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/501082026-06-18T11:13:07Z
dc.title.none.fl_str_mv A microdestructive capillary electrophoresis method for the analysis of blue-pen-ink strokes on office paper
title A microdestructive capillary electrophoresis method for the analysis of blue-pen-ink strokes on office paper
spellingShingle A microdestructive capillary electrophoresis method for the analysis of blue-pen-ink strokes on office paper
Calcerrada Guerreiro, Matías
Blue ink
blue pen
capillary electrophoresis
dyes
ink differentiation
profiling
Química
Chemistry
title_short A microdestructive capillary electrophoresis method for the analysis of blue-pen-ink strokes on office paper
title_full A microdestructive capillary electrophoresis method for the analysis of blue-pen-ink strokes on office paper
title_fullStr A microdestructive capillary electrophoresis method for the analysis of blue-pen-ink strokes on office paper
title_full_unstemmed A microdestructive capillary electrophoresis method for the analysis of blue-pen-ink strokes on office paper
title_sort A microdestructive capillary electrophoresis method for the analysis of blue-pen-ink strokes on office paper
dc.creator.none.fl_str_mv Calcerrada Guerreiro, Matías
García Ruiz, Carmen|||0000-0001-5925-3449
González Herráez, Miguel|||0000-0003-2555-2971
author Calcerrada Guerreiro, Matías
author_facet Calcerrada Guerreiro, Matías
García Ruiz, Carmen|||0000-0001-5925-3449
González Herráez, Miguel|||0000-0003-2555-2971
author_role author
author2 García Ruiz, Carmen|||0000-0001-5925-3449
González Herráez, Miguel|||0000-0003-2555-2971
author2_role author
author
dc.subject.none.fl_str_mv Blue ink
blue pen
capillary electrophoresis
dyes
ink differentiation
profiling
Química
Chemistry
topic Blue ink
blue pen
capillary electrophoresis
dyes
ink differentiation
profiling
Química
Chemistry
description This manuscript describes the development of a capillary electrophoresis (CE) method for the detection of acid and basic dyes and its application to real samples, blue-pen-ink strokes onoffice paper. First, a capillary zone electrophoresis (CZE) method was developed for the separation of basic and acid dyes, by studying the separation medium (buffer nature, pH andrelative amount of additive) and instrumental parameters (temperature, voltage and capillary dimensions). The method performance was evaluated in terms of selectivity, resolution (above 5 and 2 for acid dyes and basic dyes, respectively, except for two basic dye standards), LOD (lower than 0.4 mg/L) and precision as intraday and interday RSD values of peak migrationtimes (lower than 0.6 %). The developed method was then applied to 34 blue pens from different technologies (rollerball, ballpoint, markers) and with different ink composition (gel, 30 water-based, oil-based). A microdestructive sample treatment using a scalpel to scratch 0.3 mg of ink stroke was performed. The entire electropherogram profile allowed the visual discrimination between different types of ink and brands, being not necessary a statistical treatment. A 100% of discrimination was achieved between pen technologies, brands, and models, although non-reproducible zones in the electropherograms were found for blue gel pen samples. The two different batches of blue oil-based pens were also differentiated. Thus, this method provides a simple, microdestructive, and rapid analysis of different blue pentechnologies which may complement the current analysis of questioned documents performed by forensic laboratories.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-06-26
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/50108
https://dx.doi.org/10.1016/j.chroma.2015.04.036
url http://hdl.handle.net/10017/50108
https://dx.doi.org/10.1016/j.chroma.2015.04.036
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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