Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer
To apply a systems pathology-based approach to the quantification of nuclear Active β-catenin and human leukocyte antigen class I, and assess the biomarker involvement in a cohort of prostate tumor patients. The systems pathology approach applied allows a precise quantification of the marker express...
| Autores: | , , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2019 |
| País: | España |
| Institución: | Universitat Autònoma de Barcelona |
| Repositorio: | Dipòsit Digital de Documents de la UAB |
| Idioma: | inglés |
| OAI Identifier: | oai:ddd.uab.cat:226330 |
| Acceso en línea: | https://ddd.uab.cat/record/226330 https://dx.doi.org/urn:doi:10.1186/s13104-019-4100-z |
| Access Level: | acceso abierto |
| Palabra clave: | Active β-catenin HLA class1 Wnt/β-catenin pathway Prostate cancer Systems pathology |
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Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancerPuig Rosell, PereErill, Nadina|||0000-0003-3530-2669Terricabras, MartaSubirana, Isaac|||0000-0003-1676-0197González-García, JuditAsensi-Puig, AdriàDonovan, Michael J.Mengual, LourdesAgulló-Ortuño, M. TeresaOlivan, Mireia|||0000-0002-8464-0827Alcaraz, Antonio|||0000-0003-2612-5250Lopez-Martin, Jose A.|||0000-0001-7530-3207de Torres, Inés|||0000-0002-5495-9140Rodríguez-Peralto, José LuisRodríguez Antolín, AlfredoMorote Robles, Juan|||0000-0002-2168-323XGonzález-Rumayor, Víctor|||0000-0002-3205-1502Active β-cateninHLA class1Wnt/β-catenin pathwayProstate cancerSystems pathologyTo apply a systems pathology-based approach to the quantification of nuclear Active β-catenin and human leukocyte antigen class I, and assess the biomarker involvement in a cohort of prostate tumor patients. The systems pathology approach applied allows a precise quantification of the marker expression in the different cell compartments as well as the determination of the areas that coexpress two markers. Our data shows that the accumulation of β-catenin in the nuclear compartment is significantly decreased in the adjacent normal areas when compared to tumor of the same patients (p < 0.001). In conclusion, the application of this novel multiple immunofluorescence assay demonstrates that the upregulation of Active β-catenin is a relatively common feature of prostate tumor development, and further supports the activation of the Wnt/β-catenin pathway in prostate cancer progression. The online version of this article (10.1186/s13104-019-4100-z) contains supplementary material, which is available to authorized users.Universitat Autònoma de Barcelona 22019-01-0120192019-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/226330https://dx.doi.org/urn:doi:10.1186/s13104-019-4100-zreponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 IPT-2012-1311-300000open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2263302026-06-06T12:50:31Z |
| dc.title.none.fl_str_mv |
Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer |
| title |
Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer |
| spellingShingle |
Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer Puig Rosell, Pere Active β-catenin HLA class1 Wnt/β-catenin pathway Prostate cancer Systems pathology |
| title_short |
Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer |
| title_full |
Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer |
| title_fullStr |
Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer |
| title_full_unstemmed |
Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer |
| title_sort |
Multiple immunofluorescence assay identifies upregulation of Active β-catenin in prostate cancer |
| dc.creator.none.fl_str_mv |
Puig Rosell, Pere Erill, Nadina|||0000-0003-3530-2669 Terricabras, Marta Subirana, Isaac|||0000-0003-1676-0197 González-García, Judit Asensi-Puig, Adrià Donovan, Michael J. Mengual, Lourdes Agulló-Ortuño, M. Teresa Olivan, Mireia|||0000-0002-8464-0827 Alcaraz, Antonio|||0000-0003-2612-5250 Lopez-Martin, Jose A.|||0000-0001-7530-3207 de Torres, Inés|||0000-0002-5495-9140 Rodríguez-Peralto, José Luis Rodríguez Antolín, Alfredo Morote Robles, Juan|||0000-0002-2168-323X González-Rumayor, Víctor|||0000-0002-3205-1502 |
| author |
Puig Rosell, Pere |
| author_facet |
Puig Rosell, Pere Erill, Nadina|||0000-0003-3530-2669 Terricabras, Marta Subirana, Isaac|||0000-0003-1676-0197 González-García, Judit Asensi-Puig, Adrià Donovan, Michael J. Mengual, Lourdes Agulló-Ortuño, M. Teresa Olivan, Mireia|||0000-0002-8464-0827 Alcaraz, Antonio|||0000-0003-2612-5250 Lopez-Martin, Jose A.|||0000-0001-7530-3207 de Torres, Inés|||0000-0002-5495-9140 Rodríguez-Peralto, José Luis Rodríguez Antolín, Alfredo Morote Robles, Juan|||0000-0002-2168-323X González-Rumayor, Víctor|||0000-0002-3205-1502 |
| author_role |
author |
| author2 |
Erill, Nadina|||0000-0003-3530-2669 Terricabras, Marta Subirana, Isaac|||0000-0003-1676-0197 González-García, Judit Asensi-Puig, Adrià Donovan, Michael J. Mengual, Lourdes Agulló-Ortuño, M. Teresa Olivan, Mireia|||0000-0002-8464-0827 Alcaraz, Antonio|||0000-0003-2612-5250 Lopez-Martin, Jose A.|||0000-0001-7530-3207 de Torres, Inés|||0000-0002-5495-9140 Rodríguez-Peralto, José Luis Rodríguez Antolín, Alfredo Morote Robles, Juan|||0000-0002-2168-323X González-Rumayor, Víctor|||0000-0002-3205-1502 |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universitat Autònoma de Barcelona |
| dc.subject.none.fl_str_mv |
Active β-catenin HLA class1 Wnt/β-catenin pathway Prostate cancer Systems pathology |
| topic |
Active β-catenin HLA class1 Wnt/β-catenin pathway Prostate cancer Systems pathology |
| description |
To apply a systems pathology-based approach to the quantification of nuclear Active β-catenin and human leukocyte antigen class I, and assess the biomarker involvement in a cohort of prostate tumor patients. The systems pathology approach applied allows a precise quantification of the marker expression in the different cell compartments as well as the determination of the areas that coexpress two markers. Our data shows that the accumulation of β-catenin in the nuclear compartment is significantly decreased in the adjacent normal areas when compared to tumor of the same patients (p < 0.001). In conclusion, the application of this novel multiple immunofluorescence assay demonstrates that the upregulation of Active β-catenin is a relatively common feature of prostate tumor development, and further supports the activation of the Wnt/β-catenin pathway in prostate cancer progression. The online version of this article (10.1186/s13104-019-4100-z) contains supplementary material, which is available to authorized users. |
| publishDate |
2019 |
| dc.date.none.fl_str_mv |
2 2019-01-01 2019 2019-01-01 |
| dc.type.none.fl_str_mv |
Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://ddd.uab.cat/record/226330 https://dx.doi.org/urn:doi:10.1186/s13104-019-4100-z |
| url |
https://ddd.uab.cat/record/226330 https://dx.doi.org/urn:doi:10.1186/s13104-019-4100-z |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.relation.none.fl_str_mv |
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 IPT-2012-1311-300000 |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
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open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/ |
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openAccess |
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application/pdf |
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reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona |
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Universitat Autònoma de Barcelona |
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Dipòsit Digital de Documents de la UAB |
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Dipòsit Digital de Documents de la UAB |
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