Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice

Leishmania amazonensis parasites are etiological agents of cutaneous leishmaniasis in the New World. BALB/c mice are highly susceptible to L. amazonensis challenge due to their inability to mount parasite-dependent IFN-γ-mediated responses. Here, we analyzed the capacity of a single administration o...

Descripción completa

Detalles Bibliográficos
Autores: Soto, Manuel, Ramírez, Laura, Solana, José Carlos, Cook, Emma C. L., Hernández García, Elena, Requena, José María, Iborra Martín, Salvador
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/7359
Acceso en línea:https://hdl.handle.net/20.500.14352/7359
Access Level:acceso abierto
Palabra clave:612.017
579
Leishmania amazonensis
Live vaccines
Attenuated parasites
Murine leishmaniasis
BALB/c mice
IFN-γ
Medicina
Inmunología
Microbiología (Farmacia)
32 Ciencias Médicas
2412 Inmunología
3302.03 Microbiología Industrial
id ES_6c2db3de46c0fed045e29100dcf8cc66
oai_identifier_str oai:docta.ucm.es:20.500.14352/7359
network_acronym_str ES
network_name_str España
repository_id_str
spelling Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c MiceSoto, ManuelRamírez, LauraSolana, José CarlosCook, Emma C. L.Hernández García, ElenaRequena, José MaríaIborra Martín, Salvador612.017579Leishmania amazonensisLive vaccinesAttenuated parasitesMurine leishmaniasisBALB/c miceIFN-γMedicinaInmunologíaMicrobiología (Farmacia)32 Ciencias Médicas2412 Inmunología3302.03 Microbiología IndustrialLeishmania amazonensis parasites are etiological agents of cutaneous leishmaniasis in the New World. BALB/c mice are highly susceptible to L. amazonensis challenge due to their inability to mount parasite-dependent IFN-γ-mediated responses. Here, we analyzed the capacity of a single administration of the LiΔHSP70-II genetically-modified attenuated L. infantum line in preventing cutaneous leishmaniasis in mice challenged with L. amazonensis virulent parasites. In previous studies, this live attenuated vaccine has demonstrated to induce long-protection against murine leishmaniasis due to Old World Leishmania species. Vaccinated mice showed a reduction in the disease evolution due to L. amazonensis challenge, namely reduction in cutaneous lesions and parasite burdens. In contrast to control animals, after the challenge, protected mice showed anti-Leishmania IgG2a circulating antibodies accompanied to the induction of Leishmania-driven specific IFN-γ systemic response. An analysis performed in the lymph node draining the site of infection revealed an increase of the parasite-specific IFN-ϒ production by CD4+ and CD8+ T cells and a decrease in the secretion of IL-10 against leishmanial antigens. Since the immunity caused by the inoculation of this live vaccine generates protection against different forms of murine leishmaniasis, we postulate LiΔHSP70-II as a candidate for the development of human vaccines.MDPIUniversidad Complutense de Madrid20212021-01-0120212021-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/7359reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/73592026-06-02T12:44:21Z
dc.title.none.fl_str_mv Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice
title Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice
spellingShingle Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice
Soto, Manuel
612.017
579
Leishmania amazonensis
Live vaccines
Attenuated parasites
Murine leishmaniasis
BALB/c mice
IFN-γ
Medicina
Inmunología
Microbiología (Farmacia)
32 Ciencias Médicas
2412 Inmunología
3302.03 Microbiología Industrial
title_short Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice
title_full Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice
title_fullStr Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice
title_full_unstemmed Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice
title_sort Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice
dc.creator.none.fl_str_mv Soto, Manuel
Ramírez, Laura
Solana, José Carlos
Cook, Emma C. L.
Hernández García, Elena
Requena, José María
Iborra Martín, Salvador
author Soto, Manuel
author_facet Soto, Manuel
Ramírez, Laura
Solana, José Carlos
Cook, Emma C. L.
Hernández García, Elena
Requena, José María
Iborra Martín, Salvador
author_role author
author2 Ramírez, Laura
Solana, José Carlos
Cook, Emma C. L.
Hernández García, Elena
Requena, José María
Iborra Martín, Salvador
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 612.017
579
Leishmania amazonensis
Live vaccines
Attenuated parasites
Murine leishmaniasis
BALB/c mice
IFN-γ
Medicina
Inmunología
Microbiología (Farmacia)
32 Ciencias Médicas
2412 Inmunología
3302.03 Microbiología Industrial
topic 612.017
579
Leishmania amazonensis
Live vaccines
Attenuated parasites
Murine leishmaniasis
BALB/c mice
IFN-γ
Medicina
Inmunología
Microbiología (Farmacia)
32 Ciencias Médicas
2412 Inmunología
3302.03 Microbiología Industrial
description Leishmania amazonensis parasites are etiological agents of cutaneous leishmaniasis in the New World. BALB/c mice are highly susceptible to L. amazonensis challenge due to their inability to mount parasite-dependent IFN-γ-mediated responses. Here, we analyzed the capacity of a single administration of the LiΔHSP70-II genetically-modified attenuated L. infantum line in preventing cutaneous leishmaniasis in mice challenged with L. amazonensis virulent parasites. In previous studies, this live attenuated vaccine has demonstrated to induce long-protection against murine leishmaniasis due to Old World Leishmania species. Vaccinated mice showed a reduction in the disease evolution due to L. amazonensis challenge, namely reduction in cutaneous lesions and parasite burdens. In contrast to control animals, after the challenge, protected mice showed anti-Leishmania IgG2a circulating antibodies accompanied to the induction of Leishmania-driven specific IFN-γ systemic response. An analysis performed in the lymph node draining the site of infection revealed an increase of the parasite-specific IFN-ϒ production by CD4+ and CD8+ T cells and a decrease in the secretion of IL-10 against leishmanial antigens. Since the immunity caused by the inoculation of this live vaccine generates protection against different forms of murine leishmaniasis, we postulate LiΔHSP70-II as a candidate for the development of human vaccines.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/7359
url https://hdl.handle.net/20.500.14352/7359
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución 3.0 España
https://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410249776037888
score 15,301603

Similares en LA Referencia