Parámetros hematológicos de Rana perezi (Amphibia: Salientia)
As part of a wider research on respiratory properties of Rana perezi blood, the hematological parameters of this species have been measured. The study of blood O ,-transporting proteins (hemoglobin, Hb) and plasmatic proteins has also been initiated. Using fifteen females weighting 235 g (15.3-31.8)...
| Autores: | , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 1985 |
| País: | España |
| Institución: | Universidad de Murcia |
| Repositorio: | DIGITUM. Depósito Digital Institucional de la Universidad de Murcia |
| OAI Identifier: | oai:digitum.um.es:10201/1785 |
| Acceso en línea: | http://hdl.handle.net/10201/1785 |
| Access Level: | acceso abierto |
| Palabra clave: | Hematología Rana perezi Amphibia salientia CDU::5 - Ciencias puras y naturales::59 - Zoología |
| Sumario: | As part of a wider research on respiratory properties of Rana perezi blood, the hematological parameters of this species have been measured. The study of blood O ,-transporting proteins (hemoglobin, Hb) and plasmatic proteins has also been initiated. Using fifteen females weighting 235 g (15.3-31.8), !he following results have been obtaíned: hematocrit (He(): 25. 7 :+:3.2 (ne, 15): Red blood cell count (RBC): 482:+: f 54x 1()3/mm1 (n=I4); Hb concentration: 5. 19:+:0.99 g/100 mi (n=9); mean corpuscYlar volume (MCV): 533.29 μ3; mean corpuscular Hb concentration (MCHC): 20.15% and mean corpuscular Hb (MCH): 107.5 pg. The electrophoresis of the Hb in 5% polyacrylamide gels showed two bands. Their quantification gave 72.38:+: ±20.19% (n=ll) for the anodic band and 27.62:t20.19 (n=ll) for the catodic one. Electrophoresis of plasma was performed in different kinds of polyacrylamide gels. the best obtained being in 7.5 and 8.5% gel. Electrophoretic behavíour of plasma proteins seems to be similar to that described in terrestrial vertebrates. These results are discussed and compared with data from other species belonging to Bufo. Hy/a and Rana genera. |
|---|