Endo-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity

The biological activity of chitooligosaccharides (COS) has made them targets for industrial and medical sectors. In this work, endo-chitinase Chit33 from Trichoderma harzianum CECT 2413 was expressed in Pichia pastoris GS115 to levels never achieved before (630 mg/L; 3.3 U/mL), without its biochemic...

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Detalhes bibliográficos
Autores: Kidibule, Peter Elias, Santos-Moriano, Paloma, Plou, Francisco Jose, Fernández Lobato, María
Formato: artículo
Fecha de publicación:2020
País:España
Recursos:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:repositorio.uam.es:10486/710145
Acesso em linha:http://hdl.handle.net/10486/710145
https://dx.doi.org/10.1016/j.btre.2020.e00500
Access Level:acceso abierto
Palavra-chave:Bioactive chito-oligomers
Chitin
Chitosan
Heterologous expression
Pichia pastoris
Biología y Biomedicina / Biología
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spelling Endo-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activityKidibule, Peter EliasSantos-Moriano, PalomaPlou, Francisco JoseFernández Lobato, MaríaBioactive chito-oligomersChitinChitosanHeterologous expressionPichia pastorisBiología y Biomedicina / BiologíaThe biological activity of chitooligosaccharides (COS) has made them targets for industrial and medical sectors. In this work, endo-chitinase Chit33 from Trichoderma harzianum CECT 2413 was expressed in Pichia pastoris GS115 to levels never achieved before (630 mg/L; 3.3 U/mL), without its biochemical characteristics being substantially affected. Chit33 produced a mixture of fully and partially acetylated COS from different chitin derivatives. HPAEC-PAD Chromatography and mass spectrometry analyses showed that (GlcNAc)4 and GlcN-(GlcNAc)2 were mainly produced from colloidal chitin and chitosan, respectively. COS in reaction mixtures were fragmented according to their size and their antioxidant activity analyzed by reducing power and free radical scavenging activity essays. The highest antioxidant activity was achieved with COS in the range of 0.5−2 and 2−10 kDa produced from colloidal chitin and chitosan, respectively, which gives biotechnological potential to both the chitin derivatives of 0.5−10 kDa and the biocatalyst producing themThis work was supported by Spanish Ministry of Economy and Competitiveness [BIO2016-76601-C3-1/-2], Fundación Ramón Areces [XIX Call of Research Grants in Life and Material Sciences], EU EMFF-Blue Economy-2018 [Fish4Fish-863697] and by an institutional grant from Fundación Ramón Areces to the Centro de Biología Molecular Severo OchoaElsevierDepartamento de Biología MolecularFacultad de Ciencias20202020-07-03research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/710145https://dx.doi.org/10.1016/j.btre.2020.e00500reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7101452026-06-23T12:46:27Z
dc.title.none.fl_str_mv Endo-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity
title Endo-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity
spellingShingle Endo-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity
Kidibule, Peter Elias
Bioactive chito-oligomers
Chitin
Chitosan
Heterologous expression
Pichia pastoris
Biología y Biomedicina / Biología
title_short Endo-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity
title_full Endo-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity
title_fullStr Endo-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity
title_full_unstemmed Endo-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity
title_sort Endo-chitinase Chit33 specificity on different chitinolytic materials allows the production of unexplored chitooligosaccharides with antioxidant activity
dc.creator.none.fl_str_mv Kidibule, Peter Elias
Santos-Moriano, Paloma
Plou, Francisco Jose
Fernández Lobato, María
author Kidibule, Peter Elias
author_facet Kidibule, Peter Elias
Santos-Moriano, Paloma
Plou, Francisco Jose
Fernández Lobato, María
author_role author
author2 Santos-Moriano, Paloma
Plou, Francisco Jose
Fernández Lobato, María
author2_role author
author
author
dc.contributor.none.fl_str_mv Departamento de Biología Molecular
Facultad de Ciencias
dc.subject.none.fl_str_mv Bioactive chito-oligomers
Chitin
Chitosan
Heterologous expression
Pichia pastoris
Biología y Biomedicina / Biología
topic Bioactive chito-oligomers
Chitin
Chitosan
Heterologous expression
Pichia pastoris
Biología y Biomedicina / Biología
description The biological activity of chitooligosaccharides (COS) has made them targets for industrial and medical sectors. In this work, endo-chitinase Chit33 from Trichoderma harzianum CECT 2413 was expressed in Pichia pastoris GS115 to levels never achieved before (630 mg/L; 3.3 U/mL), without its biochemical characteristics being substantially affected. Chit33 produced a mixture of fully and partially acetylated COS from different chitin derivatives. HPAEC-PAD Chromatography and mass spectrometry analyses showed that (GlcNAc)4 and GlcN-(GlcNAc)2 were mainly produced from colloidal chitin and chitosan, respectively. COS in reaction mixtures were fragmented according to their size and their antioxidant activity analyzed by reducing power and free radical scavenging activity essays. The highest antioxidant activity was achieved with COS in the range of 0.5−2 and 2−10 kDa produced from colloidal chitin and chitosan, respectively, which gives biotechnological potential to both the chitin derivatives of 0.5−10 kDa and the biocatalyst producing them
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-07-03
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/710145
https://dx.doi.org/10.1016/j.btre.2020.e00500
url http://hdl.handle.net/10486/710145
https://dx.doi.org/10.1016/j.btre.2020.e00500
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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