Replication data for "A Notch trans-activation to cis-inhibition switch underlies hematopoietic stem cell aging"
This dataset contains the code used to analyse the single-cell RNA sequencing (scRNA-seq) data, the raw count matrices and all the output obtained from running the code from the manuscript "A Notch trans-activation to cis-inhibition switch underlies hematopoietic stem cell aging" by Mattei...
| Autores: | , , , , , , , , , , , , |
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| Tipo de recurso: | conjunto de datos |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Consorci de Serveis Universitaris de Catalunya (CSUC) |
| Repositorio: | CORA.Repositori de Dades de Recerca |
| OAI Identifier: | oai:dnet:cora.rdr____::35948447cbc4af5f6357764c270ac0a5 |
| Acceso en línea: | https://doi.org/10.34810/DATA1340 |
| Access Level: | acceso abierto |
| Palabra clave: | Medicine, Health and Life Sciences Notch2 Jag2 Hematopoietic Stem Cells bone marrow mortality/aging |
| Sumario: | This dataset contains the code used to analyse the single-cell RNA sequencing (scRNA-seq) data, the raw count matrices and all the output obtained from running the code from the manuscript "A Notch trans-activation to cis-inhibition switch underlies hematopoietic stem cell aging" by Matteini et al. scRNA-seq was applied on sorted Lin- Sca1+ cKit+ (LSK) cells from the bone marrow of young and aged C57Bl6 mice and the raw FASTQ files can be found under the GEO accession numbers GSE197070 and GSE269953 (only young and aged controls used in this analysis). The code is in an easily reproducible format, as an Rmarkdown notebook that contains comments that describe all the steps in the analysis. To start reproducing it, download all the files, open the "LSK_scRNAseq_data_analysis.Rmd" file in an Rstudio session and start following its instructions. This repository also contains the supplementary and raw data included in the manuscript. |
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