Early metabolic alterations in pediatric obesity: Depot-specific insights from untargeted adipose tissue metabolomics.

BACKGROUND: Childhood obesity is associated with lifelong metabolic risk, yet depot-specific alterations in adipose tissue metabolism during early life remain poorly understood. OBJECTIVE: This study aimed to characterize the metabolic differences between subcutaneous (sWAT) and visceral (vWAT) whit...

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Autores: López-Gonzálvez Á, Muñoz-San Martín C, Soria-Gondek A, Requena B, Fernández-García P, González L, Reyes-Farias M, Murillo M, Valls A, Corrales P, Villarroya F, Cereijo R, Herrero L, Barbas C, Sánchez-Infantes D, Gonzalez-Riano C
Tipo de recurso: artículo
Fecha de publicación:2026
País:España
Institución:Fundació Sant Joan de Déu
Repositorio:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
OAI Identifier:oai:dnet:r-fsjd______::d6da37bdd9b3cda82955b635e8160d18
Acceso en línea:https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=30189
Access Level:acceso abierto
Palabra clave:Adipose tissue
CE-MS
LC-MS
Pediatric obesity
Untargeted metabolomics
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spelling Early metabolic alterations in pediatric obesity: Depot-specific insights from untargeted adipose tissue metabolomics.López-Gonzálvez ÁMuñoz-San Martín CSoria-Gondek ARequena BFernández-García PGonzález LReyes-Farias MMurillo MValls ACorrales PVillarroya FCereijo RHerrero LBarbas CSánchez-Infantes DGonzalez-Riano CAdipose tissueCE-MSLC-MSPediatric obesityUntargeted metabolomicsBACKGROUND: Childhood obesity is associated with lifelong metabolic risk, yet depot-specific alterations in adipose tissue metabolism during early life remain poorly understood. OBJECTIVE: This study aimed to characterize the metabolic differences between subcutaneous (sWAT) and visceral (vWAT) white adipose tissue in pediatric obesity using untargeted metabolomics. METHODS: Adipose tissue samples were collected from 12 children with overweight/obesity (OW/OB) and 18 who were of normal weight (NW). Untargeted metabolomics was performed using capillary electrophoresis-mass spectrometry to profile polar metabolites in sWAT and vWAT, and free fatty acids (FFAs) were analyzed using liquid chromatography-mass spectrometry. RESULTS: Comparison of children with OW/OB vs NW revealed pronounced depot-specific heterogeneity. vWAT in children with OW/OB exhibited 24 significantly altered metabolites compared with NW controls. This visceral profile was characterized by elevated ketone bodies (3-hydroxybutyrate and acetoacetic acid), tricarboxylic acid cycle intermediates (citric and pyruvic acids), and long-chain FFAs (palmitic and oleic acids). Concurrently, amino acid imbalances, specifically elevated leucine and arginine but reduced histidine and carnosine, suggested heightened mitochondrial stress and inflammation. In contrast, sWAT from children with OW/OB showed fewer variations (12 metabolites), defined primarily by elevated glutamate, leucine, and short-chain FFAs, reflecting a milder metabolic disruption. Direct comparison between depots revealed that vWAT was enriched in amino acids and carnitine, while sWAT showed relatively higher levels of glycolytic and ketone body intermediates in NW conditions. CONCLUSION: Depot-specific metabolic differences are evident in pediatric obesity. vWAT in children with OW/OB displays a metabolic profile consistent with heightened lipotoxicity and mitochondrial stress, whereas sWAT exhibits fewer, less pronounced metabolic differences.ELSEVIER SCIENCE INC2026info:eu-repo/semantics/articleaheadOfPrintVersionhttps://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=30189Journal of Clinical LipidologyISSN: 19332874ISSNe: 18764789reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déuinstname:Fundació Sant Joan de DéuInglésinfo:eu-repo/semantics/openAccessoai:dnet:r-fsjd______::d6da37bdd9b3cda82955b635e8160d182026-05-27T12:37:41Z
dc.title.none.fl_str_mv Early metabolic alterations in pediatric obesity: Depot-specific insights from untargeted adipose tissue metabolomics.
title Early metabolic alterations in pediatric obesity: Depot-specific insights from untargeted adipose tissue metabolomics.
spellingShingle Early metabolic alterations in pediatric obesity: Depot-specific insights from untargeted adipose tissue metabolomics.
López-Gonzálvez Á
Adipose tissue
CE-MS
LC-MS
Pediatric obesity
Untargeted metabolomics
title_short Early metabolic alterations in pediatric obesity: Depot-specific insights from untargeted adipose tissue metabolomics.
title_full Early metabolic alterations in pediatric obesity: Depot-specific insights from untargeted adipose tissue metabolomics.
title_fullStr Early metabolic alterations in pediatric obesity: Depot-specific insights from untargeted adipose tissue metabolomics.
title_full_unstemmed Early metabolic alterations in pediatric obesity: Depot-specific insights from untargeted adipose tissue metabolomics.
title_sort Early metabolic alterations in pediatric obesity: Depot-specific insights from untargeted adipose tissue metabolomics.
dc.creator.none.fl_str_mv López-Gonzálvez Á
Muñoz-San Martín C
Soria-Gondek A
Requena B
Fernández-García P
González L
Reyes-Farias M
Murillo M
Valls A
Corrales P
Villarroya F
Cereijo R
Herrero L
Barbas C
Sánchez-Infantes D
Gonzalez-Riano C
author López-Gonzálvez Á
author_facet López-Gonzálvez Á
Muñoz-San Martín C
Soria-Gondek A
Requena B
Fernández-García P
González L
Reyes-Farias M
Murillo M
Valls A
Corrales P
Villarroya F
Cereijo R
Herrero L
Barbas C
Sánchez-Infantes D
Gonzalez-Riano C
author_role author
author2 Muñoz-San Martín C
Soria-Gondek A
Requena B
Fernández-García P
González L
Reyes-Farias M
Murillo M
Valls A
Corrales P
Villarroya F
Cereijo R
Herrero L
Barbas C
Sánchez-Infantes D
Gonzalez-Riano C
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adipose tissue
CE-MS
LC-MS
Pediatric obesity
Untargeted metabolomics
topic Adipose tissue
CE-MS
LC-MS
Pediatric obesity
Untargeted metabolomics
description BACKGROUND: Childhood obesity is associated with lifelong metabolic risk, yet depot-specific alterations in adipose tissue metabolism during early life remain poorly understood. OBJECTIVE: This study aimed to characterize the metabolic differences between subcutaneous (sWAT) and visceral (vWAT) white adipose tissue in pediatric obesity using untargeted metabolomics. METHODS: Adipose tissue samples were collected from 12 children with overweight/obesity (OW/OB) and 18 who were of normal weight (NW). Untargeted metabolomics was performed using capillary electrophoresis-mass spectrometry to profile polar metabolites in sWAT and vWAT, and free fatty acids (FFAs) were analyzed using liquid chromatography-mass spectrometry. RESULTS: Comparison of children with OW/OB vs NW revealed pronounced depot-specific heterogeneity. vWAT in children with OW/OB exhibited 24 significantly altered metabolites compared with NW controls. This visceral profile was characterized by elevated ketone bodies (3-hydroxybutyrate and acetoacetic acid), tricarboxylic acid cycle intermediates (citric and pyruvic acids), and long-chain FFAs (palmitic and oleic acids). Concurrently, amino acid imbalances, specifically elevated leucine and arginine but reduced histidine and carnosine, suggested heightened mitochondrial stress and inflammation. In contrast, sWAT from children with OW/OB showed fewer variations (12 metabolites), defined primarily by elevated glutamate, leucine, and short-chain FFAs, reflecting a milder metabolic disruption. Direct comparison between depots revealed that vWAT was enriched in amino acids and carnitine, while sWAT showed relatively higher levels of glycolytic and ketone body intermediates in NW conditions. CONCLUSION: Depot-specific metabolic differences are evident in pediatric obesity. vWAT in children with OW/OB displays a metabolic profile consistent with heightened lipotoxicity and mitochondrial stress, whereas sWAT exhibits fewer, less pronounced metabolic differences.
publishDate 2026
dc.date.none.fl_str_mv 2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
aheadOfPrintVersion
format article
dc.identifier.none.fl_str_mv https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=30189
url https://fsjd.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=30189
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ELSEVIER SCIENCE INC
publisher.none.fl_str_mv ELSEVIER SCIENCE INC
dc.source.none.fl_str_mv Journal of Clinical Lipidology
ISSN: 19332874
ISSNe: 18764789
reponame:r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
instname:Fundació Sant Joan de Déu
instname_str Fundació Sant Joan de Déu
reponame_str r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
collection r-FSJD. Repositorio Institucional de Producción Científica de la Fundació Sant Joan de Déu
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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