Extracellular Vesicles Secreted by Hypoxic AC10 Cardiomyocytes Modulate Fibroblast Cell Motility

[EN] Extracellular vesicles (EVs) are small membrane vesicles secreted by most cell types with important roles in cell-to-cell communication. To assess their relevance in the context of heart ischemia, EVs isolated from the AC10 ventricular cardiomyocyte cell line (CM-EVs), exposed to normoxia (Nx)...

Full description

Bibliographic Details
Authors: Ontoria-Oviedo, Imelda, Dorronsoro, Akaitz, Sánchez, Rafael, Ciria, María, Gómez-Ferrer, Marta, Buiges, Marc, Grueso, Elena, Tejedor, Sandra, García-García, Francisco, González-King, Hernán, Garcia, Nahuel A., Peiró-Molina, Esteban, Sepúlveda, Pilar
Format: article
Publication Date:2018
Country:España
Institution:Universitat Politècnica de València (UPV)
Repository:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Language:English
OAI Identifier:oai:riunet.upv.es:10251/204070
Online Access:https://riunet.upv.es/handle/10251/204070
Access Level:Open access
Keyword:Cardiomyocytes
Fibroblasts
Endothelial cells
Extracellular vesicles
Hypoxia
Cellular communication
id ES_6bacc627e8dbdaf6b0fee6fef6feacad
oai_identifier_str oai:riunet.upv.es:10251/204070
network_acronym_str ES
network_name_str España
repository_id_str
spelling Extracellular Vesicles Secreted by Hypoxic AC10 Cardiomyocytes Modulate Fibroblast Cell MotilityOntoria-Oviedo, ImeldaDorronsoro, AkaitzSánchez, RafaelCiria, MaríaGómez-Ferrer, MartaBuiges, MarcGrueso, ElenaTejedor, SandraGarcía-García, FranciscoGonzález-King, HernánGarcia, Nahuel A.Peiró-Molina, EstebanSepúlveda, PilarCardiomyocytesFibroblastsEndothelial cellsExtracellular vesiclesHypoxiaCellular communication[EN] Extracellular vesicles (EVs) are small membrane vesicles secreted by most cell types with important roles in cell-to-cell communication. To assess their relevance in the context of heart ischemia, EVs isolated from the AC10 ventricular cardiomyocyte cell line (CM-EVs), exposed to normoxia (Nx) or hypoxia (Hx), were incubated with fibroblasts (Fb) and endothelial cells (EC). CM-EVs were studied using electron microscopy, nanoparticle tracking analysis (NTA), western blotting and proteomic analysis. Results showed that EVs had a strong preference to be internalized by EC over fibroblasts, suggesting an active exosome-based communication mechanism between CM and EC in the heart. In Matrigel tube-formation assays, Hx CM-EVs were inferior to Nx CM-EVs in angiogenesis. By contrast, in a wound-healing assay, wound closure was faster in fibroblasts treated with Hx CM-EVs than with Nx CM-EVs, supporting a pro-fibrotic effect of Hx CM-EVs. Overall, these observations were consistent with the different protein cargoes detected by proteomic analysis under Nx and Hx conditions and the biological pathways identified. The paracrine crosstalk between CM-EVs, Fb, and EC in different physiological conditions could account for the contribution of CM-EVs to cardiac remodeling after an ischemic insult.This work was supported by grants PI16/0107 and RETICS Program (RD16/0011/0004) from Instituto de Salud Carlos III cofunded by the European Regional Development Fund ERDF una manera de hacer Europa. The proteomic studies were carried out in the University of Valencia Proteomics Unit, a member of the ISCIII ProteoRed Proteomics Platform. The bioinformatics analysis was performed in the Bioinformatic and Biostatistics Unit of the Principe Felipe Research Center using the computational infrastructure supported by ERDF.Frontiers Media SAInstituto de Salud Carlos IIIEuropean Regional Development FundMinisterio de Economía y CompetitividadRepositorio Institucional de la Universitat Politècnica de València Riunet20182018-10-25journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/204070reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 RD16%2F0011%2F0004 Red de Terapia Celular (TerCel)Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI16%2F0107open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2040702026-06-13T07:49:27Z
dc.title.none.fl_str_mv Extracellular Vesicles Secreted by Hypoxic AC10 Cardiomyocytes Modulate Fibroblast Cell Motility
title Extracellular Vesicles Secreted by Hypoxic AC10 Cardiomyocytes Modulate Fibroblast Cell Motility
spellingShingle Extracellular Vesicles Secreted by Hypoxic AC10 Cardiomyocytes Modulate Fibroblast Cell Motility
Ontoria-Oviedo, Imelda
Cardiomyocytes
Fibroblasts
Endothelial cells
Extracellular vesicles
Hypoxia
Cellular communication
title_short Extracellular Vesicles Secreted by Hypoxic AC10 Cardiomyocytes Modulate Fibroblast Cell Motility
title_full Extracellular Vesicles Secreted by Hypoxic AC10 Cardiomyocytes Modulate Fibroblast Cell Motility
title_fullStr Extracellular Vesicles Secreted by Hypoxic AC10 Cardiomyocytes Modulate Fibroblast Cell Motility
title_full_unstemmed Extracellular Vesicles Secreted by Hypoxic AC10 Cardiomyocytes Modulate Fibroblast Cell Motility
title_sort Extracellular Vesicles Secreted by Hypoxic AC10 Cardiomyocytes Modulate Fibroblast Cell Motility
dc.creator.none.fl_str_mv Ontoria-Oviedo, Imelda
Dorronsoro, Akaitz
Sánchez, Rafael
Ciria, María
Gómez-Ferrer, Marta
Buiges, Marc
Grueso, Elena
Tejedor, Sandra
García-García, Francisco
González-King, Hernán
Garcia, Nahuel A.
Peiró-Molina, Esteban
Sepúlveda, Pilar
author Ontoria-Oviedo, Imelda
author_facet Ontoria-Oviedo, Imelda
Dorronsoro, Akaitz
Sánchez, Rafael
Ciria, María
Gómez-Ferrer, Marta
Buiges, Marc
Grueso, Elena
Tejedor, Sandra
García-García, Francisco
González-King, Hernán
Garcia, Nahuel A.
Peiró-Molina, Esteban
Sepúlveda, Pilar
author_role author
author2 Dorronsoro, Akaitz
Sánchez, Rafael
Ciria, María
Gómez-Ferrer, Marta
Buiges, Marc
Grueso, Elena
Tejedor, Sandra
García-García, Francisco
González-King, Hernán
Garcia, Nahuel A.
Peiró-Molina, Esteban
Sepúlveda, Pilar
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
European Regional Development Fund
Ministerio de Economía y Competitividad
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Cardiomyocytes
Fibroblasts
Endothelial cells
Extracellular vesicles
Hypoxia
Cellular communication
topic Cardiomyocytes
Fibroblasts
Endothelial cells
Extracellular vesicles
Hypoxia
Cellular communication
description [EN] Extracellular vesicles (EVs) are small membrane vesicles secreted by most cell types with important roles in cell-to-cell communication. To assess their relevance in the context of heart ischemia, EVs isolated from the AC10 ventricular cardiomyocyte cell line (CM-EVs), exposed to normoxia (Nx) or hypoxia (Hx), were incubated with fibroblasts (Fb) and endothelial cells (EC). CM-EVs were studied using electron microscopy, nanoparticle tracking analysis (NTA), western blotting and proteomic analysis. Results showed that EVs had a strong preference to be internalized by EC over fibroblasts, suggesting an active exosome-based communication mechanism between CM and EC in the heart. In Matrigel tube-formation assays, Hx CM-EVs were inferior to Nx CM-EVs in angiogenesis. By contrast, in a wound-healing assay, wound closure was faster in fibroblasts treated with Hx CM-EVs than with Nx CM-EVs, supporting a pro-fibrotic effect of Hx CM-EVs. Overall, these observations were consistent with the different protein cargoes detected by proteomic analysis under Nx and Hx conditions and the biological pathways identified. The paracrine crosstalk between CM-EVs, Fb, and EC in different physiological conditions could account for the contribution of CM-EVs to cardiac remodeling after an ischemic insult.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-10-25
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/204070
url https://riunet.upv.es/handle/10251/204070
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 RD16%2F0011%2F0004 Red de Terapia Celular (TerCel)
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI16%2F0107
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media SA
publisher.none.fl_str_mv Frontiers Media SA
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410213807783936
score 15,301603

Similar Items