The dynamics of mitochondrial Ca2+ fluxes

Producción Científica

Detalles Bibliográficos
Autores: Fuente Pérez, Sergio De La, Montenegro, Pablo, Fonteriz García, Rosalba Inés, Moreno Díaz-Calderón, Alfredo, Domínguez Lobatón, María Carmen, Montero Zoccola, María Teresa, Álvarez Martín, Javier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2014
País:España
Institución:Universidad de Valladolid
Repositorio:UVaDOC. Repositorio Documental de la Universidad de Valladolid
OAI Identifier:oai:uvadoc.uva.es:10324/67171
Acceso en línea:https://doi.org/10.1016/j.bbabio.2010.06.008
https://uvadoc.uva.es/handle/10324/67171
Access Level:acceso abierto
Palabra clave:Ca2+ fluxes
Ca2+ dynamics
Ca2+ buffering
Mitochondria
Aequorin
id ES_6baa8aac6a2a1ac07cf83c19f7cc0d2b
oai_identifier_str oai:uvadoc.uva.es:10324/67171
network_acronym_str ES
network_name_str España
repository_id_str
spelling The dynamics of mitochondrial Ca2+ fluxesFuente Pérez, Sergio De LaMontenegro, PabloFonteriz García, Rosalba InésMoreno Díaz-Calderón, AlfredoDomínguez Lobatón, María CarmenMontero Zoccola, María TeresaÁlvarez Martín, JavierCa2+ fluxesCa2+ dynamicsCa2+ bufferingMitochondriaAequorinProducción CientíficaMICU1 (Ca2+ uptake protein 1, mitochondrial) is an important regulator of the MCU (Ca2+ uniporter protein, mitochondrial) that has been shown recently to act as a gatekeeper of the MCU at low [Ca2+]c (cytosolic [Ca2+]). In the present study we have investigated in detail the dynamics of MCU activity after shRNA-knockdown of MICU1 and we have found several new interesting properties. In MICU1-knockdown cells, the rate of mitochondrial Ca2+ uptake was largely increased at a low [Ca2+]c (<2 μM), but it was decreased at a high [Ca2+]c (>4 μM). In the 2-4 μM range a mixed behaviour was observed, where mitochondrial Ca2+ uptake started earlier in the MICU1-silenced cells, but at a lower rate than in the controls. The sensitivity of Ca2+ uptake to Ruthenium Red and Ru360 was similar at both high and low [Ca2+]c, indicating that the same Ca2+ pathway was operating in both cases. The increased Ca2+-uptake rate observed at a [Ca2+]c below 2 μM was transient and became inhibited during Ca2+ entry. Development of this inhibition was slow, requiring 5 min for completion, and was hardly reversible. Therefore MICU1 acts both as a MCU gatekeeper at low [Ca2+]c and as a cofactor necessary to reach the maximum Ca2+-uptake rate at high [Ca2+]c. Moreover, in the absence of MICU1, the MCU becomes sensitive to a slow-developing inhibition that requires prolonged increases in [Ca2+]c in the low micromolar range.Elsevier2014info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1016/j.bbabio.2010.06.008https://uvadoc.uva.es/handle/10324/67171reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolidinstname:Universidad de ValladolidInglésinfo:eu-repo/semantics/openAccessoai:uvadoc.uva.es:10324/671712026-06-13T12:44:47Z
dc.title.none.fl_str_mv The dynamics of mitochondrial Ca2+ fluxes
title The dynamics of mitochondrial Ca2+ fluxes
spellingShingle The dynamics of mitochondrial Ca2+ fluxes
Fuente Pérez, Sergio De La
Ca2+ fluxes
Ca2+ dynamics
Ca2+ buffering
Mitochondria
Aequorin
title_short The dynamics of mitochondrial Ca2+ fluxes
title_full The dynamics of mitochondrial Ca2+ fluxes
title_fullStr The dynamics of mitochondrial Ca2+ fluxes
title_full_unstemmed The dynamics of mitochondrial Ca2+ fluxes
title_sort The dynamics of mitochondrial Ca2+ fluxes
dc.creator.none.fl_str_mv Fuente Pérez, Sergio De La
Montenegro, Pablo
Fonteriz García, Rosalba Inés
Moreno Díaz-Calderón, Alfredo
Domínguez Lobatón, María Carmen
Montero Zoccola, María Teresa
Álvarez Martín, Javier
author Fuente Pérez, Sergio De La
author_facet Fuente Pérez, Sergio De La
Montenegro, Pablo
Fonteriz García, Rosalba Inés
Moreno Díaz-Calderón, Alfredo
Domínguez Lobatón, María Carmen
Montero Zoccola, María Teresa
Álvarez Martín, Javier
author_role author
author2 Montenegro, Pablo
Fonteriz García, Rosalba Inés
Moreno Díaz-Calderón, Alfredo
Domínguez Lobatón, María Carmen
Montero Zoccola, María Teresa
Álvarez Martín, Javier
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ca2+ fluxes
Ca2+ dynamics
Ca2+ buffering
Mitochondria
Aequorin
topic Ca2+ fluxes
Ca2+ dynamics
Ca2+ buffering
Mitochondria
Aequorin
description Producción Científica
publishDate 2014
dc.date.none.fl_str_mv 2014
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1016/j.bbabio.2010.06.008
https://uvadoc.uva.es/handle/10324/67171
url https://doi.org/10.1016/j.bbabio.2010.06.008
https://uvadoc.uva.es/handle/10324/67171
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:UVaDOC. Repositorio Documental de la Universidad de Valladolid
instname:Universidad de Valladolid
instname_str Universidad de Valladolid
reponame_str UVaDOC. Repositorio Documental de la Universidad de Valladolid
collection UVaDOC. Repositorio Documental de la Universidad de Valladolid
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410213303418880
score 15.811543

Similares en LA Referencia