miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2.

Age related macular degeneration (AMD) is a common retina-related disease leading to blindness. Little is known on the origin of the disease, but it is well documented that oxidative stress generated in the retinal pigment epithelium and choroid neovascularization are closely involved. The study of...

Descripción completa

Detalles Bibliográficos
Autores: Oltra, Maria, Vidal-Gil, Lorena, Maisto, Rosa, Oltra, Sara S, Romero, Francisco Javier, Sancho-Pelluz, Javier, Barcia, Jorge Miguel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:INCLIVA
Repositorio:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
OAI Identifier:oai:incliva.fundanetsuite.com:p4051
Acceso en línea:https://incliva.portalinvestigacion.com/publicaciones/4051
Access Level:acceso abierto
id ES_6b772492780583dcd67d5faec4e2f1db
oai_identifier_str oai:incliva.fundanetsuite.com:p4051
network_acronym_str ES
network_name_str España
repository_id_str
spelling miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2.Oltra, MariaVidal-Gil, LorenaMaisto, RosaOltra, Sara SRomero, Francisco JavierSancho-Pelluz, JavierBarcia, Jorge MiguelAge related macular degeneration (AMD) is a common retina-related disease leading to blindness. Little is known on the origin of the disease, but it is well documented that oxidative stress generated in the retinal pigment epithelium and choroid neovascularization are closely involved. The study of circulating miRNAs is opening new possibilities in terms of diagnosis and therapeutics. miRNAs can travel associated to lipoproteins or inside small Extracellular Vesicles (sEVs). A number of reports indicate a significant deregulation of circulating miRNAs in AMD and experimental approaches, but it is unclear whether sEVs present a significant miRNA cargo. The present work studies miRNA expression changes in sEVs released from ARPE-19 cells under oxidative conditions (i.e. hydrogen peroxide, H2O2). H2O2 increased sEVs release from ARPE-19 cells. Moreover, 218 miRNAs could be detected in control and H2O2 induced-sEVs. Interestingly, only two of them (hsa-miR-302a and hsa-miR-122) were significantly under-expressed in H2O2-induced sEVs. Results herein suggest that the down regulation of miRNAs 302a and 122 might be related with previous studies showing sEVs-induced neovascularization after oxidative challenge in ARPE-19 cells.NATURE PORTFOLIO2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://incliva.portalinvestigacion.com/publicaciones/4051Scientific ReportsISSN: 20452322reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVAinstname:INCLIVAInglésinfo:eu-repo/semantics/openAccessoai:incliva.fundanetsuite.com:p40512026-06-07T16:35:31Z
dc.title.none.fl_str_mv miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2.
title miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2.
spellingShingle miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2.
Oltra, Maria
title_short miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2.
title_full miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2.
title_fullStr miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2.
title_full_unstemmed miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2.
title_sort miR302a and 122 are deregulated in small extracellular vesicles from ARPE-19 cells cultured with H2O2.
dc.creator.none.fl_str_mv Oltra, Maria
Vidal-Gil, Lorena
Maisto, Rosa
Oltra, Sara S
Romero, Francisco Javier
Sancho-Pelluz, Javier
Barcia, Jorge Miguel
author Oltra, Maria
author_facet Oltra, Maria
Vidal-Gil, Lorena
Maisto, Rosa
Oltra, Sara S
Romero, Francisco Javier
Sancho-Pelluz, Javier
Barcia, Jorge Miguel
author_role author
author2 Vidal-Gil, Lorena
Maisto, Rosa
Oltra, Sara S
Romero, Francisco Javier
Sancho-Pelluz, Javier
Barcia, Jorge Miguel
author2_role author
author
author
author
author
author
description Age related macular degeneration (AMD) is a common retina-related disease leading to blindness. Little is known on the origin of the disease, but it is well documented that oxidative stress generated in the retinal pigment epithelium and choroid neovascularization are closely involved. The study of circulating miRNAs is opening new possibilities in terms of diagnosis and therapeutics. miRNAs can travel associated to lipoproteins or inside small Extracellular Vesicles (sEVs). A number of reports indicate a significant deregulation of circulating miRNAs in AMD and experimental approaches, but it is unclear whether sEVs present a significant miRNA cargo. The present work studies miRNA expression changes in sEVs released from ARPE-19 cells under oxidative conditions (i.e. hydrogen peroxide, H2O2). H2O2 increased sEVs release from ARPE-19 cells. Moreover, 218 miRNAs could be detected in control and H2O2 induced-sEVs. Interestingly, only two of them (hsa-miR-302a and hsa-miR-122) were significantly under-expressed in H2O2-induced sEVs. Results herein suggest that the down regulation of miRNAs 302a and 122 might be related with previous studies showing sEVs-induced neovascularization after oxidative challenge in ARPE-19 cells.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://incliva.portalinvestigacion.com/publicaciones/4051
url https://incliva.portalinvestigacion.com/publicaciones/4051
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv NATURE PORTFOLIO
publisher.none.fl_str_mv NATURE PORTFOLIO
dc.source.none.fl_str_mv Scientific Reports
ISSN: 20452322
reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
instname:INCLIVA
instname_str INCLIVA
reponame_str r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
collection r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410197997355008
score 15.811543

Similares en LA Referencia