An In Vitro Protocol for Propagating Castanea sativa Italian Cultivars

Castanea sativa cv. ‘Garrone Rosso’ and ‘Marrone di Castel del Rio’ are two of the most prized varieties in Italy due to their valuable and healthy nuts used for fresh consumption and in the confectionery industry. Despite the growing demand for chestnuts, there are constraints regarding plant propa...

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Detalhes bibliográficos
Autores: Pavese, Vera, Ruffa, P., Abbà, S., Costa, R. L., Corredoira, Elena, Silvestri, C., Torello Marinoni, D., Botta, Roberto
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/304104
Acesso em linha:http://hdl.handle.net/10261/304104
Access Level:acceso abierto
Palavra-chave:Axillary budding
Chestnut
In vitro culture
Micropropagation
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spelling An In Vitro Protocol for Propagating Castanea sativa Italian CultivarsPavese, VeraRuffa, P.Abbà, S.Costa, R. L.Corredoira, ElenaSilvestri, C.Torello Marinoni, D.Botta, RobertoAxillary buddingChestnutIn vitro cultureMicropropagationCastanea sativa cv. ‘Garrone Rosso’ and ‘Marrone di Castel del Rio’ are two of the most prized varieties in Italy due to their valuable and healthy nuts used for fresh consumption and in the confectionery industry. Despite the growing demand for chestnuts, there are constraints regarding plant propagation that hamper the renewal and new planting of orchards in different areas. Castanea sativa is susceptible to diseases that have caused a reduction in its area of production. For this reason, in vitro culture represents a valuable technique for germplasm preservation and plant multiplication enabling production of a high number of plants for use in breeding programs. Here we present an in vitro micropropagation protocol for Italian Castanea sativa cv. ‘Marrone di Castel del Rio’ and cv. ‘Garrone Rosso’ to contribute to the preservation and enhancement of the Italian germplasm. Nodal explants were used as the starting material for in vitro establishment. The cv. ‘Marrone di Castel del Rio’ showed a high percentage of survival explants (92%) when subjected to long bleach exposure (25 min), in contrast to what was observed for the ‘Garrone Rosso’ cultivar. Ascorbic acid was found to be the best compound to counteract phenol exudation. The MS3B and DKW media supplied with 0.5 mg/L BAP were effective for in vitro establishment, while the DKW medium (0.1 mg/L BAP and 0.05 mg/L IBA) was preferable for the proliferation phase. A double-layer rooting methodology was used and 35% rooting was observed with 25 mg/L IBA rooting treatment.This research was funded by Fondazione CRT (RF.2018.2549).Multidisciplinary Digital Publishing InstituteFondazione Cassa di Risparmio di TorinoConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320222023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/304104reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.3390/plants11233308Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3041042026-05-22T06:33:51Z
dc.title.none.fl_str_mv An In Vitro Protocol for Propagating Castanea sativa Italian Cultivars
title An In Vitro Protocol for Propagating Castanea sativa Italian Cultivars
spellingShingle An In Vitro Protocol for Propagating Castanea sativa Italian Cultivars
Pavese, Vera
Axillary budding
Chestnut
In vitro culture
Micropropagation
title_short An In Vitro Protocol for Propagating Castanea sativa Italian Cultivars
title_full An In Vitro Protocol for Propagating Castanea sativa Italian Cultivars
title_fullStr An In Vitro Protocol for Propagating Castanea sativa Italian Cultivars
title_full_unstemmed An In Vitro Protocol for Propagating Castanea sativa Italian Cultivars
title_sort An In Vitro Protocol for Propagating Castanea sativa Italian Cultivars
dc.creator.none.fl_str_mv Pavese, Vera
Ruffa, P.
Abbà, S.
Costa, R. L.
Corredoira, Elena
Silvestri, C.
Torello Marinoni, D.
Botta, Roberto
author Pavese, Vera
author_facet Pavese, Vera
Ruffa, P.
Abbà, S.
Costa, R. L.
Corredoira, Elena
Silvestri, C.
Torello Marinoni, D.
Botta, Roberto
author_role author
author2 Ruffa, P.
Abbà, S.
Costa, R. L.
Corredoira, Elena
Silvestri, C.
Torello Marinoni, D.
Botta, Roberto
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fondazione Cassa di Risparmio di Torino
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Axillary budding
Chestnut
In vitro culture
Micropropagation
topic Axillary budding
Chestnut
In vitro culture
Micropropagation
description Castanea sativa cv. ‘Garrone Rosso’ and ‘Marrone di Castel del Rio’ are two of the most prized varieties in Italy due to their valuable and healthy nuts used for fresh consumption and in the confectionery industry. Despite the growing demand for chestnuts, there are constraints regarding plant propagation that hamper the renewal and new planting of orchards in different areas. Castanea sativa is susceptible to diseases that have caused a reduction in its area of production. For this reason, in vitro culture represents a valuable technique for germplasm preservation and plant multiplication enabling production of a high number of plants for use in breeding programs. Here we present an in vitro micropropagation protocol for Italian Castanea sativa cv. ‘Marrone di Castel del Rio’ and cv. ‘Garrone Rosso’ to contribute to the preservation and enhancement of the Italian germplasm. Nodal explants were used as the starting material for in vitro establishment. The cv. ‘Marrone di Castel del Rio’ showed a high percentage of survival explants (92%) when subjected to long bleach exposure (25 min), in contrast to what was observed for the ‘Garrone Rosso’ cultivar. Ascorbic acid was found to be the best compound to counteract phenol exudation. The MS3B and DKW media supplied with 0.5 mg/L BAP were effective for in vitro establishment, while the DKW medium (0.1 mg/L BAP and 0.05 mg/L IBA) was preferable for the proliferation phase. A double-layer rooting methodology was used and 35% rooting was observed with 25 mg/L IBA rooting treatment.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/304104
url http://hdl.handle.net/10261/304104
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.3390/plants11233308
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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