First record of paralytic shellfish toxins in marine pufferfish from the Spanish Mediterranean coast using cell-based assay, automated patch clamp and HPLC-FLD

Pufferfish is one of the most poisonous marine organisms, responsible for numerous poisoning incidents and some human fatalities due to its capability to accumulate potent neurotoxins such as tetrodotoxins (TTXs) and paralytic shellfish toxins (PSTs). In this study, tissue extracts (muscle, skin, li...

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Autores: Alkassar, Mounira, Tudó Casanova, Àngels, Rambla Alegre, Maria, Ferreres, Laura, Diogène, Jorge, Sureda, Francesc X., Campàs, Mònica
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:20.500.12327/3290
Acceso en línea:http://hdl.handle.net/20.500.12327/3290
https://doi.org/10.1016/j.chemosphere.2024.143053
Access Level:acceso abierto
Palabra clave:574
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oai_identifier_str oai:recercat.cat:20.500.12327/3290
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv First record of paralytic shellfish toxins in marine pufferfish from the Spanish Mediterranean coast using cell-based assay, automated patch clamp and HPLC-FLD
title First record of paralytic shellfish toxins in marine pufferfish from the Spanish Mediterranean coast using cell-based assay, automated patch clamp and HPLC-FLD
spellingShingle First record of paralytic shellfish toxins in marine pufferfish from the Spanish Mediterranean coast using cell-based assay, automated patch clamp and HPLC-FLD
Alkassar, Mounira
574
title_short First record of paralytic shellfish toxins in marine pufferfish from the Spanish Mediterranean coast using cell-based assay, automated patch clamp and HPLC-FLD
title_full First record of paralytic shellfish toxins in marine pufferfish from the Spanish Mediterranean coast using cell-based assay, automated patch clamp and HPLC-FLD
title_fullStr First record of paralytic shellfish toxins in marine pufferfish from the Spanish Mediterranean coast using cell-based assay, automated patch clamp and HPLC-FLD
title_full_unstemmed First record of paralytic shellfish toxins in marine pufferfish from the Spanish Mediterranean coast using cell-based assay, automated patch clamp and HPLC-FLD
title_sort First record of paralytic shellfish toxins in marine pufferfish from the Spanish Mediterranean coast using cell-based assay, automated patch clamp and HPLC-FLD
dc.creator.none.fl_str_mv Alkassar, Mounira
Tudó Casanova, Àngels
Rambla Alegre, Maria
Ferreres, Laura
Diogène, Jorge
Sureda, Francesc X.
Campàs, Mònica
author Alkassar, Mounira
author_facet Alkassar, Mounira
Tudó Casanova, Àngels
Rambla Alegre, Maria
Ferreres, Laura
Diogène, Jorge
Sureda, Francesc X.
Campàs, Mònica
author_role author
author2 Tudó Casanova, Àngels
Rambla Alegre, Maria
Ferreres, Laura
Diogène, Jorge
Sureda, Francesc X.
Campàs, Mònica
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Aigües Marines i Continentals
dc.subject.none.fl_str_mv 574
topic 574
description Pufferfish is one of the most poisonous marine organisms, responsible for numerous poisoning incidents and some human fatalities due to its capability to accumulate potent neurotoxins such as tetrodotoxins (TTXs) and paralytic shellfish toxins (PSTs). In this study, tissue extracts (muscle, skin, liver, intestinal tract and gonads) obtained from sixteen pufferfish specimens of the Lagocephalus lagocephalus and Sphoeroides pachygaster species, collected along the Spanish Mediterranean coast, were analysed for the presence of voltage-gated sodium channel (also known as Nav channel) blockers using cell-based assay (CBA) and automated patch clamp (APC). No toxicity was observed in any of the S. pachygaster specimens, but toxicity was detected in the liver of most L. lagocephalus specimens. Instrumental analysis of these specimens, as well as in one Lagocephalus sceleratus specimen, by high-performance liquid chromatography coupled to fluorescence detection (HPLC-FLD) was performed, which confirmed the presence of PSTs only in L. lagocephalus specimens. This analysis reported the presence of saxitoxin (STX) and decarbamoylsaxitoxin (dcSTX) in all positive samples, being dcSTX the major analogue. These results demonstrate the ability of this species to accumulate PSTs, being the first report of the presence of PSTs in Mediterranean L. lagocephalus specimens. Furthermore, the presence of high PSTs contents in all five tested tissues of one L. lagocephalus specimen pointed the risk that the presence of this toxic fish in the Mediterranean Sea may represent for seafood safety and human health in case of accidental consumption.
publishDate 2024
dc.date.none.fl_str_mv 2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/3290
https://doi.org/10.1016/j.chemosphere.2024.143053
url http://hdl.handle.net/20.500.12327/3290
https://doi.org/10.1016/j.chemosphere.2024.143053
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Chemosphere
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
MC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C22/ES/Herramientas biotecnológicas basadas en células y aptámeros para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRA
MICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2023-149899OB-C21/ES/Tecnologías bio-analíticas basadas en anticuerpos, células y espectrometría de masas para explorar las fronteras de las toxinas marinas/BiOCEANsing
MICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2023-149899OB-C22/ES/Tecnologías bio-analíticas basadas en pequeñas proteínas combinatorias, canales iónicos artificiales y células para explorar las fronteras de las toxinas marinas/BiOCEANsing
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 33
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
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spelling First record of paralytic shellfish toxins in marine pufferfish from the Spanish Mediterranean coast using cell-based assay, automated patch clamp and HPLC-FLDAlkassar, MouniraTudó Casanova, ÀngelsRambla Alegre, MariaFerreres, LauraDiogène, JorgeSureda, Francesc X.Campàs, Mònica574Pufferfish is one of the most poisonous marine organisms, responsible for numerous poisoning incidents and some human fatalities due to its capability to accumulate potent neurotoxins such as tetrodotoxins (TTXs) and paralytic shellfish toxins (PSTs). In this study, tissue extracts (muscle, skin, liver, intestinal tract and gonads) obtained from sixteen pufferfish specimens of the Lagocephalus lagocephalus and Sphoeroides pachygaster species, collected along the Spanish Mediterranean coast, were analysed for the presence of voltage-gated sodium channel (also known as Nav channel) blockers using cell-based assay (CBA) and automated patch clamp (APC). No toxicity was observed in any of the S. pachygaster specimens, but toxicity was detected in the liver of most L. lagocephalus specimens. Instrumental analysis of these specimens, as well as in one Lagocephalus sceleratus specimen, by high-performance liquid chromatography coupled to fluorescence detection (HPLC-FLD) was performed, which confirmed the presence of PSTs only in L. lagocephalus specimens. This analysis reported the presence of saxitoxin (STX) and decarbamoylsaxitoxin (dcSTX) in all positive samples, being dcSTX the major analogue. These results demonstrate the ability of this species to accumulate PSTs, being the first report of the presence of PSTs in Mediterranean L. lagocephalus specimens. Furthermore, the presence of high PSTs contents in all five tested tissues of one L. lagocephalus specimen pointed the risk that the presence of this toxic fish in the Mediterranean Sea may represent for seafood safety and human health in case of accidental consumption.This research was funded by the Ministerio de Ciencia e Innovación (MICIN) and the Agencia Estatal de Investigación (AEI) through the CELLECTRA (PID2020-112976RB-C21 and PID2020-112976RB-C22) and the BiOCEANsing (PID2023-149899OB-C21 and PID2023-149899OB-C22) projects. Mounira Alkassar acknowledges MICIN and AEI for her PhD grant (PRE2019-088181). Àngels Tudó akcnowledges Ministerio de Universidades (Spain) for her Margarita Salas postdoc grant (2021URV-MS-24). The authors acknowledge laboratory assistance from Ana Meseguer. The authors also acknowledge support from CERCA Program/Generalitat de Catalunya and the Direcció General de Política Marítima i Pesca Sostenible of the Government of Catalunya and Almirall laboratories.info:eu-repo/semantics/acceptedVersionElsevierProducció AnimalAigües Marines i Continentals2024info:eu-repo/semantics/article33http://hdl.handle.net/20.500.12327/3290https://doi.org/10.1016/j.chemosphere.2024.143053reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésChemosphereMC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C21/ES/Herramientas biotecnológicas basadas en células y receptores para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRAMC/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I y Programa Estatal de I+D+I orientada a los retos de la sociedad/PID2020-112976RB-C22/ES/Herramientas biotecnológicas basadas en células y aptámeros para la detección de ciguatoxinas y tetrodotoxinas/CELLECTRAMICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2023-149899OB-C21/ES/Tecnologías bio-analíticas basadas en anticuerpos, células y espectrometría de masas para explorar las fronteras de las toxinas marinas/BiOCEANsingMICINN/Programa Estatal para impulsar la investigación científico-técnica y su transferencia/PID2023-149899OB-C22/ES/Tecnologías bio-analíticas basadas en pequeñas proteínas combinatorias, canales iónicos artificiales y células para explorar las fronteras de las toxinas marinas/BiOCEANsingAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:20.500.12327/32902026-05-29T05:05:01Z
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