6-hydroxydopamine (6-OHDA) oxidative stress assay for observing dopaminergic neuron loss in caenorhabditis elegans

The nematode Caenorhabditis elegans is a powerful genetic model that can be used to investigate neuronal death. Research using C. elegans has been crucial to characterize cell death programmes that are conserved in mammals. Many neuronal signaling components, such as those mediating dopaminergic neu...

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Detalles Bibliográficos
Autores: Offenburger, Sarah-Lena, Gartner, Anton
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/43158
Acceso en línea:http://hdl.handle.net/10230/43158
http://dx.doi.org/10.21769/BioProtoc.3025
Access Level:acceso abierto
Palabra clave:6-OHDA
6-hydroxydopamine
C. elegans
Caenorhabditis elegans
Dopaminergic neurodegeneration
Intoxication
Oxidative stress assay
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spelling 6-hydroxydopamine (6-OHDA) oxidative stress assay for observing dopaminergic neuron loss in caenorhabditis elegansOffenburger, Sarah-LenaGartner, Anton6-OHDA6-hydroxydopamineC. elegansCaenorhabditis elegansDopaminergic neurodegenerationIntoxicationOxidative stress assayThe nematode Caenorhabditis elegans is a powerful genetic model that can be used to investigate neuronal death. Research using C. elegans has been crucial to characterize cell death programmes that are conserved in mammals. Many neuronal signaling components, such as those mediating dopaminergic neurotransmission, are preserved as well. Dopaminergic neurons are progressively lost in Parkinson's disease and an important risk factor to develop this disease appears to be oxidative stress, the increased occurrence of highly reactive oxygen species. Oxidative stress-induced dopaminergic neurodegeneration is mimicked in animal models by treatment with 6-hydroxydopamine (6-OHDA), a dopamine analog, which is specifically taken up into dopaminergic neurons. After exposing C. elegans to 6-OHDA, the loss of fluorescently labeled dopaminergic neurons can be easily monitored. An organisms' sensitivity to oxidative stress is thought to be influenced by basal levels of intrinsic oxidative stress and the ability to counteract oxidative stress and oxidative stress-induced damage. The C. elegans '6-OHDA model' led to the discovery of novel genes that are required to protect dopaminergic neurons and it has helped to determine the effects of conserved cell death and cell engulfment pathways in dopaminergic neurodegeneration. Here, we describe a simple protocol that allows for the easy detection of dopaminergic neuron loss after 6-OHDA treatment in C. elegans.Bio-Protocol201920192018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/43158http://dx.doi.org/10.21769/BioProtoc.3025reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésBio Protoc. 2018; 8(18). pii: e3025© 2018 The Authors; exclusive licensee Bio-protocol LLC.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/431582026-05-29T05:05:01Z
dc.title.none.fl_str_mv 6-hydroxydopamine (6-OHDA) oxidative stress assay for observing dopaminergic neuron loss in caenorhabditis elegans
title 6-hydroxydopamine (6-OHDA) oxidative stress assay for observing dopaminergic neuron loss in caenorhabditis elegans
spellingShingle 6-hydroxydopamine (6-OHDA) oxidative stress assay for observing dopaminergic neuron loss in caenorhabditis elegans
Offenburger, Sarah-Lena
6-OHDA
6-hydroxydopamine
C. elegans
Caenorhabditis elegans
Dopaminergic neurodegeneration
Intoxication
Oxidative stress assay
title_short 6-hydroxydopamine (6-OHDA) oxidative stress assay for observing dopaminergic neuron loss in caenorhabditis elegans
title_full 6-hydroxydopamine (6-OHDA) oxidative stress assay for observing dopaminergic neuron loss in caenorhabditis elegans
title_fullStr 6-hydroxydopamine (6-OHDA) oxidative stress assay for observing dopaminergic neuron loss in caenorhabditis elegans
title_full_unstemmed 6-hydroxydopamine (6-OHDA) oxidative stress assay for observing dopaminergic neuron loss in caenorhabditis elegans
title_sort 6-hydroxydopamine (6-OHDA) oxidative stress assay for observing dopaminergic neuron loss in caenorhabditis elegans
dc.creator.none.fl_str_mv Offenburger, Sarah-Lena
Gartner, Anton
author Offenburger, Sarah-Lena
author_facet Offenburger, Sarah-Lena
Gartner, Anton
author_role author
author2 Gartner, Anton
author2_role author
dc.subject.none.fl_str_mv 6-OHDA
6-hydroxydopamine
C. elegans
Caenorhabditis elegans
Dopaminergic neurodegeneration
Intoxication
Oxidative stress assay
topic 6-OHDA
6-hydroxydopamine
C. elegans
Caenorhabditis elegans
Dopaminergic neurodegeneration
Intoxication
Oxidative stress assay
description The nematode Caenorhabditis elegans is a powerful genetic model that can be used to investigate neuronal death. Research using C. elegans has been crucial to characterize cell death programmes that are conserved in mammals. Many neuronal signaling components, such as those mediating dopaminergic neurotransmission, are preserved as well. Dopaminergic neurons are progressively lost in Parkinson's disease and an important risk factor to develop this disease appears to be oxidative stress, the increased occurrence of highly reactive oxygen species. Oxidative stress-induced dopaminergic neurodegeneration is mimicked in animal models by treatment with 6-hydroxydopamine (6-OHDA), a dopamine analog, which is specifically taken up into dopaminergic neurons. After exposing C. elegans to 6-OHDA, the loss of fluorescently labeled dopaminergic neurons can be easily monitored. An organisms' sensitivity to oxidative stress is thought to be influenced by basal levels of intrinsic oxidative stress and the ability to counteract oxidative stress and oxidative stress-induced damage. The C. elegans '6-OHDA model' led to the discovery of novel genes that are required to protect dopaminergic neurons and it has helped to determine the effects of conserved cell death and cell engulfment pathways in dopaminergic neurodegeneration. Here, we describe a simple protocol that allows for the easy detection of dopaminergic neuron loss after 6-OHDA treatment in C. elegans.
publishDate 2018
dc.date.none.fl_str_mv 2018
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/43158
http://dx.doi.org/10.21769/BioProtoc.3025
url http://hdl.handle.net/10230/43158
http://dx.doi.org/10.21769/BioProtoc.3025
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Bio Protoc. 2018; 8(18). pii: e3025
dc.rights.none.fl_str_mv © 2018 The Authors; exclusive licensee Bio-protocol LLC.
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2018 The Authors; exclusive licensee Bio-protocol LLC.
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Bio-Protocol
publisher.none.fl_str_mv Bio-Protocol
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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