Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection
In this work, we present an electrochemical sensor for fast, low-cost, and easy detection of the SARS-CoV-2 spike protein in infected patients. The sensor is based on a selected combination of nanomaterials with a specific purpose. A bioconjugate formed by Few-layer bismuthene nanosheets (FLB) and t...
| Autores: | , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad Autónoma de Madrid |
| Repositorio: | Biblos-e Archivo. Repositorio Institucional de la UAM |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.uam.es:10486/713343 |
| Acceso en línea: | http://hdl.handle.net/10486/713343 https://dx.doi.org/10.1016/j.bios.2024.116500 |
| Access Level: | acceso abierto |
| Palabra clave: | Nanostructured Bioconjugate Tetrahedral DNA Nanostructure (TDN) SARS-CoV-2 Aptamer-Based Tetrahedral DNA Nanostructure (TDNapt) Aptasensor Bismuthene Nanosheets Física Química |
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España |
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| dc.title.none.fl_str_mv |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| title |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| spellingShingle |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection Enebral Romero, Estefanía Nanostructured Bioconjugate Tetrahedral DNA Nanostructure (TDN) SARS-CoV-2 Aptamer-Based Tetrahedral DNA Nanostructure (TDNapt) Aptasensor Bismuthene Nanosheets Física Química |
| title_short |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| title_full |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| title_fullStr |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| title_full_unstemmed |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| title_sort |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detection |
| dc.creator.none.fl_str_mv |
Enebral Romero, Estefanía García Fernández, Daniel Gutiérrez Gálvez, Laura López Diego, David Luna, Mónica García Martín, Adrián Salagre Rubio, Elena García Michel, Enrique Torres Peña, Iñigo Zamora Abanades, Félix Juan García Mendiola, Tania Lorenzo Abad, Encarnación |
| author |
Enebral Romero, Estefanía |
| author_facet |
Enebral Romero, Estefanía García Fernández, Daniel Gutiérrez Gálvez, Laura López Diego, David Luna, Mónica García Martín, Adrián Salagre Rubio, Elena García Michel, Enrique Torres Peña, Iñigo Zamora Abanades, Félix Juan García Mendiola, Tania Lorenzo Abad, Encarnación |
| author_role |
author |
| author2 |
García Fernández, Daniel Gutiérrez Gálvez, Laura López Diego, David Luna, Mónica García Martín, Adrián Salagre Rubio, Elena García Michel, Enrique Torres Peña, Iñigo Zamora Abanades, Félix Juan García Mendiola, Tania Lorenzo Abad, Encarnación |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Departamento de Física de la Materia Condensada Departamento de Química Analítica y Análisis Instrumental Facultad de Ciencias |
| dc.subject.none.fl_str_mv |
Nanostructured Bioconjugate Tetrahedral DNA Nanostructure (TDN) SARS-CoV-2 Aptamer-Based Tetrahedral DNA Nanostructure (TDNapt) Aptasensor Bismuthene Nanosheets Física Química |
| topic |
Nanostructured Bioconjugate Tetrahedral DNA Nanostructure (TDN) SARS-CoV-2 Aptamer-Based Tetrahedral DNA Nanostructure (TDNapt) Aptasensor Bismuthene Nanosheets Física Química |
| description |
In this work, we present an electrochemical sensor for fast, low-cost, and easy detection of the SARS-CoV-2 spike protein in infected patients. The sensor is based on a selected combination of nanomaterials with a specific purpose. A bioconjugate formed by Few-layer bismuthene nanosheets (FLB) and tetrahedral DNA nanostructures (TDNs) is immobilized on Carbon Screen-Printed Electrodes (CSPE). The TDNs contain on the top vertex an aptamer that specifically binds to the SARS-CoV-2 spike protein, and a thiol group at the three basal vertices to anchor to the FLB. The TDNs are also marked with a redox indicator, Azure A (AA), which allows the direct detection of SARS-CoV-2 spike protein through changes in the current intensity of its electrolysis before and after the biorecognition reaction. The developed sensor can detect SARS-CoV-2 spike protein with a detection limit of 1.74 fg mL 1 directly in nasopharyngeal swab human samples. Therefore, this study offers a new strategy for rapid virus detection since it is versatile enough for different viruses and pathogens |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 2024-06-13 |
| dc.type.none.fl_str_mv |
research article http://purl.org/coar/resource_type/c_2df8fbb1 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10486/713343 https://dx.doi.org/10.1016/j.bios.2024.116500 |
| url |
http://hdl.handle.net/10486/713343 https://dx.doi.org/10.1016/j.bios.2024.116500 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 Attribution-NonCommercial 4.0 International http://creativecommons.org/licenses/by-nc/4.0/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:Biblos-e Archivo. Repositorio Institucional de la UAM instname:Universidad Autónoma de Madrid |
| instname_str |
Universidad Autónoma de Madrid |
| reponame_str |
Biblos-e Archivo. Repositorio Institucional de la UAM |
| collection |
Biblos-e Archivo. Repositorio Institucional de la UAM |
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|
| repository.mail.fl_str_mv |
|
| _version_ |
1869410170174439424 |
| spelling |
Bismuthene - Tetrahedral DNA nanobioconjugate for virus detectionEnebral Romero, EstefaníaGarcía Fernández, DanielGutiérrez Gálvez, LauraLópez Diego, DavidLuna, MónicaGarcía Martín, AdriánSalagre Rubio, ElenaGarcía Michel, EnriqueTorres Peña, IñigoZamora Abanades, Félix JuanGarcía Mendiola, TaniaLorenzo Abad, EncarnaciónNanostructured BioconjugateTetrahedral DNA Nanostructure (TDN)SARS-CoV-2 Aptamer-Based Tetrahedral DNANanostructure (TDNapt)AptasensorBismuthene NanosheetsFísicaQuímicaIn this work, we present an electrochemical sensor for fast, low-cost, and easy detection of the SARS-CoV-2 spike protein in infected patients. The sensor is based on a selected combination of nanomaterials with a specific purpose. A bioconjugate formed by Few-layer bismuthene nanosheets (FLB) and tetrahedral DNA nanostructures (TDNs) is immobilized on Carbon Screen-Printed Electrodes (CSPE). The TDNs contain on the top vertex an aptamer that specifically binds to the SARS-CoV-2 spike protein, and a thiol group at the three basal vertices to anchor to the FLB. The TDNs are also marked with a redox indicator, Azure A (AA), which allows the direct detection of SARS-CoV-2 spike protein through changes in the current intensity of its electrolysis before and after the biorecognition reaction. The developed sensor can detect SARS-CoV-2 spike protein with a detection limit of 1.74 fg mL 1 directly in nasopharyngeal swab human samples. Therefore, this study offers a new strategy for rapid virus detection since it is versatile enough for different viruses and pathogensThis work has been financially supported by the Spanish Ministry of Economy and Competitiveness (PID2020-116728RB-I00, RED2022- 134120-T), Spanish Ministry of Science, Innovation and Universities (TED2021-129738B–I00) and Community of Madrid (REACT-UE NANOCOV-CM, TRANSNANOAVANSENS, S2018/NMT-4349). Estefanía Enebral-Romero thanks the financial support of “Nanotecnología para detecci´ on del SARS-CoV-2 y sus variantes. NANOCOV” project and a contract as a predoctoral researcher funded by grant CEX2020- 001039-S, supported by MCIN/AEI/10.13039/501100011033. Laura Guti´ errez-Gálvez was supported by a Formación del Profesorado Universitario (FPU) grant from the Spanish Ministry of Universities (FPU19/ 06309). Daniel García-Fernández and Adrián García-Martín were supported by a Programa Investigo grant from Autonomous Community of Madrid in the framework of the Plan de Recuperación, Transformación y Resiliencia financed by the European Union – NextGenerationEU (09- PIN1-00013.4/2022- 83rd and 66rd place, respectively). The authors acknowledge the support of the European Union (EU) and Horizon 2020 through Instruct Proposal PID: 21859, and the CRIOMECORR project (ESFRI-2019-01-CSIC-16) to the cryoEM CNB-CSIC facility. F. Z. acknowledges financial support from the Spanish Ministry of Science and Innovation, through the “María de Maeztu” Programme for Units of Excellence in R&D (CEX2023-001316-M, PDC2021-120782- C21, and PID2022-138908NB-C31), the “(MAD2D-CM)-UAM” project funded by Comunidad de Madrid, by the Recovery, Transformation and Resilience Plan, and by NextGenerationEU from the European Union. F. Z. also acknowledge support from the European Innovation Council under grant Agreement 101047081 (EVA). EGM acknowledges financial support from the Spanish Ministry of Science and Innovation, through the “María de Maeztu” Programme for Units of Excellence in R&D (CEX2018-000805-M) and through grant PID2021-123295NB-I00. We also acknowledge María U. Gonz´ alez help with Fluorescence Microscopy and the service from the MiNa Laboratory at IMN, and funding from CM (project S2018/NMT-4291 TEC2SPACE), MINECO (project CSIC13-4E-1794) and EU (FEDER, FSE)ElsevierDepartamento de Física de la Materia CondensadaDepartamento de Química Analítica y Análisis InstrumentalFacultad de Ciencias20242024-06-13research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/713343https://dx.doi.org/10.1016/j.bios.2024.116500reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial 4.0 Internationalhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/7133432026-06-23T12:46:27Z |
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15,300719 |