Effect of the solvent on the chromatographic selectivity in reversed-phase and HILIC

In this work, the characterization of several reversed-phase and HILIC chromatographic systems is presented by means of the Abraham's solvation parameter model, focusing on the impact of solute polarizability, dipolarity, hydrogen bonding, and molecular volume on chromatographic retention. Alth...

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Detalles Bibliográficos
Autores: Subirats i Vila, Xavier, Casanovas, Laura, Redón, Lídia, Rosés Pascual, Martí
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/199472
Acceso en línea:https://hdl.handle.net/2445/199472
Access Level:acceso abierto
Palabra clave:Cromatografia de líquids
Dissolvents
Solvatació
Liquid chromatography
Solvents
Solvation
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spelling Effect of the solvent on the chromatographic selectivity in reversed-phase and HILICSubirats i Vila, XavierCasanovas, LauraRedón, LídiaRosés Pascual, MartíCromatografia de líquidsDissolventsSolvatacióLiquid chromatographySolventsSolvationIn this work, the characterization of several reversed-phase and HILIC chromatographic systems is presented by means of the Abraham's solvation parameter model, focusing on the impact of solute polarizability, dipolarity, hydrogen bonding, and molecular volume on chromatographic retention. Although retention times in octadecylsilane columns are clearly dependent on the nature and content of the organic modifier in the mobile phase, similar chromatographic selectivities are reported for eluents containing acetonitrile or methanol in the range between 40 and 80%. The most relevant analyte properties affecting retention are the hydrogen bond acceptor capacity and the molecular volume, the former favoring partition into the mobile phase and the latter into the stationary phase. The behavior of HILIC systems greatly depends on the nature of the support (silica or polymeric), the bonded phase (zwitterionic, aminopropyl, dihydroxypropyl) and the organic solvent used in the eluent (acetonitrile or methanol), but they have in common that larger solute volumes allow more favorable partition into the organic solvent-rich mobile phase. The evaluation of the chromatographic retention of ionized analytes in HILIC should be performed with care, since they may interact with ionized buffering species, leading to unexpected lower retentions.Elsevier B.V.2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/199472Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.sampre.2023.100063Advances in Sample Preparation, 2023, vol. 6, p. 100063https://doi.org/10.1016/j.sampre.2023.100063cc-by (c) Subirats i Vila, Xavier et al., 2023https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1994722026-05-27T06:46:51Z
dc.title.none.fl_str_mv Effect of the solvent on the chromatographic selectivity in reversed-phase and HILIC
title Effect of the solvent on the chromatographic selectivity in reversed-phase and HILIC
spellingShingle Effect of the solvent on the chromatographic selectivity in reversed-phase and HILIC
Subirats i Vila, Xavier
Cromatografia de líquids
Dissolvents
Solvatació
Liquid chromatography
Solvents
Solvation
title_short Effect of the solvent on the chromatographic selectivity in reversed-phase and HILIC
title_full Effect of the solvent on the chromatographic selectivity in reversed-phase and HILIC
title_fullStr Effect of the solvent on the chromatographic selectivity in reversed-phase and HILIC
title_full_unstemmed Effect of the solvent on the chromatographic selectivity in reversed-phase and HILIC
title_sort Effect of the solvent on the chromatographic selectivity in reversed-phase and HILIC
dc.creator.none.fl_str_mv Subirats i Vila, Xavier
Casanovas, Laura
Redón, Lídia
Rosés Pascual, Martí
author Subirats i Vila, Xavier
author_facet Subirats i Vila, Xavier
Casanovas, Laura
Redón, Lídia
Rosés Pascual, Martí
author_role author
author2 Casanovas, Laura
Redón, Lídia
Rosés Pascual, Martí
author2_role author
author
author
dc.subject.none.fl_str_mv Cromatografia de líquids
Dissolvents
Solvatació
Liquid chromatography
Solvents
Solvation
topic Cromatografia de líquids
Dissolvents
Solvatació
Liquid chromatography
Solvents
Solvation
description In this work, the characterization of several reversed-phase and HILIC chromatographic systems is presented by means of the Abraham's solvation parameter model, focusing on the impact of solute polarizability, dipolarity, hydrogen bonding, and molecular volume on chromatographic retention. Although retention times in octadecylsilane columns are clearly dependent on the nature and content of the organic modifier in the mobile phase, similar chromatographic selectivities are reported for eluents containing acetonitrile or methanol in the range between 40 and 80%. The most relevant analyte properties affecting retention are the hydrogen bond acceptor capacity and the molecular volume, the former favoring partition into the mobile phase and the latter into the stationary phase. The behavior of HILIC systems greatly depends on the nature of the support (silica or polymeric), the bonded phase (zwitterionic, aminopropyl, dihydroxypropyl) and the organic solvent used in the eluent (acetonitrile or methanol), but they have in common that larger solute volumes allow more favorable partition into the organic solvent-rich mobile phase. The evaluation of the chromatographic retention of ionized analytes in HILIC should be performed with care, since they may interact with ionized buffering species, leading to unexpected lower retentions.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/199472
url https://hdl.handle.net/2445/199472
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1016/j.sampre.2023.100063
Advances in Sample Preparation, 2023, vol. 6, p. 100063
https://doi.org/10.1016/j.sampre.2023.100063
dc.rights.none.fl_str_mv cc-by (c) Subirats i Vila, Xavier et al., 2023
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Subirats i Vila, Xavier et al., 2023
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Química i Química Analítica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.300719

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