PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis

Eukaryotic cells divide and separate all their components after chromosome segregation by a process called cytokinesis to complete cell division. Cytokinesis is highly regulated by the recruitment of the components to the division site and through post-translational modifications such as phosphoryla...

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Detalles Bibliográficos
Autores: Moyano Rodríguez, Yolanda, 1992-, Vaquero Pelaz, David, Vilalta Castany, Odena, Foltman, Magdalena, Sánchez Díaz, Alberto, Queralt Badia, Ethel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/184461
Acceso en línea:https://hdl.handle.net/2445/184461
Access Level:acceso abierto
Palabra clave:Citogenètica
Sacaromicetàcies
Cytogenetics
Saccharomycetaceae
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spelling PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesisMoyano Rodríguez, Yolanda, 1992-Vaquero Pelaz, DavidVilalta Castany, OdenaFoltman, MagdalenaSánchez Díaz, AlbertoQueralt Badia, EthelCitogenèticaSacaromicetàciesCytogeneticsSaccharomycetaceaeEukaryotic cells divide and separate all their components after chromosome segregation by a process called cytokinesis to complete cell division. Cytokinesis is highly regulated by the recruitment of the components to the division site and through post-translational modifications such as phosphorylations. The budding yeast mitotic kinases Cdc28-Clb2, Cdc5, and Dbf2-Mob1 phosphorylate several cytokinetic proteins contributing to the regulation of cytokinesis. The PP2A-Cdc55 phosphatase regulates mitosis counteracting Cdk1- and Cdc5-dependent phosphorylation. This prompted us to propose that PP2A-Cdc55 could also be counteracting the mitotic kinases during cytokinesis. Here we show that in the absence of Cdc55, AMR contraction and the primary septum formation occur asymmetrically to one side of the bud neck supporting a role for PP2A-Cdc55 in cytokinesis regulation. In addition, by in vivo and in vitro assays, we show that PP2A-Cdc55 dephosphorylates the chitin synthase II (Chs2 in budding yeast) a component of the Ingression Progression Complexes (IPCs) involved in cytokinesis. Interestingly, the non-phosphorylable version of Chs2 rescues the asymmetric AMR contraction and the defective septa formation observed in cdc55 increment mutant cells. Therefore, timely dephosphorylation of the Chs2 by PP2A-Cdc55 is crucial for proper actomyosin ring contraction. These findings reveal a new mechanism of cytokinesis regulation by the PP2A-Cdc55 phosphatase and extend our knowledge of the involvement of multiple phosphatases during cytokinesis.Springer Science and Business Media LLC2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/184461Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1007/s00018-022-04209-1Cellular and Molecular Life Sciences, 2022, vol 79, num 3https://doi.org/10.1007/s00018-022-04209-1cc by (c) Moyano Rodríguez, Yolanda et al, 2022http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1844612026-05-27T06:46:51Z
dc.title.none.fl_str_mv PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis
title PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis
spellingShingle PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis
Moyano Rodríguez, Yolanda, 1992-
Citogenètica
Sacaromicetàcies
Cytogenetics
Saccharomycetaceae
title_short PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis
title_full PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis
title_fullStr PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis
title_full_unstemmed PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis
title_sort PP2A-Cdc55 phosphatase regulates actomyosin ring contraction and septum formation during cytokinesis
dc.creator.none.fl_str_mv Moyano Rodríguez, Yolanda, 1992-
Vaquero Pelaz, David
Vilalta Castany, Odena
Foltman, Magdalena
Sánchez Díaz, Alberto
Queralt Badia, Ethel
author Moyano Rodríguez, Yolanda, 1992-
author_facet Moyano Rodríguez, Yolanda, 1992-
Vaquero Pelaz, David
Vilalta Castany, Odena
Foltman, Magdalena
Sánchez Díaz, Alberto
Queralt Badia, Ethel
author_role author
author2 Vaquero Pelaz, David
Vilalta Castany, Odena
Foltman, Magdalena
Sánchez Díaz, Alberto
Queralt Badia, Ethel
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Citogenètica
Sacaromicetàcies
Cytogenetics
Saccharomycetaceae
topic Citogenètica
Sacaromicetàcies
Cytogenetics
Saccharomycetaceae
description Eukaryotic cells divide and separate all their components after chromosome segregation by a process called cytokinesis to complete cell division. Cytokinesis is highly regulated by the recruitment of the components to the division site and through post-translational modifications such as phosphorylations. The budding yeast mitotic kinases Cdc28-Clb2, Cdc5, and Dbf2-Mob1 phosphorylate several cytokinetic proteins contributing to the regulation of cytokinesis. The PP2A-Cdc55 phosphatase regulates mitosis counteracting Cdk1- and Cdc5-dependent phosphorylation. This prompted us to propose that PP2A-Cdc55 could also be counteracting the mitotic kinases during cytokinesis. Here we show that in the absence of Cdc55, AMR contraction and the primary septum formation occur asymmetrically to one side of the bud neck supporting a role for PP2A-Cdc55 in cytokinesis regulation. In addition, by in vivo and in vitro assays, we show that PP2A-Cdc55 dephosphorylates the chitin synthase II (Chs2 in budding yeast) a component of the Ingression Progression Complexes (IPCs) involved in cytokinesis. Interestingly, the non-phosphorylable version of Chs2 rescues the asymmetric AMR contraction and the defective septa formation observed in cdc55 increment mutant cells. Therefore, timely dephosphorylation of the Chs2 by PP2A-Cdc55 is crucial for proper actomyosin ring contraction. These findings reveal a new mechanism of cytokinesis regulation by the PP2A-Cdc55 phosphatase and extend our knowledge of the involvement of multiple phosphatases during cytokinesis.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/184461
url https://hdl.handle.net/2445/184461
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1007/s00018-022-04209-1
Cellular and Molecular Life Sciences, 2022, vol 79, num 3
https://doi.org/10.1007/s00018-022-04209-1
dc.rights.none.fl_str_mv cc by (c) Moyano Rodríguez, Yolanda et al, 2022
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Moyano Rodríguez, Yolanda et al, 2022
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Science and Business Media LLC
publisher.none.fl_str_mv Springer Science and Business Media LLC
dc.source.none.fl_str_mv Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL))
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301603

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