Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics

Nitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S...

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Detalhes bibliográficos
Autores: Santos, Ana Isabel, Lourenço, Ana Sofía, Simão, Sónia, Marques da Silva, Dorinda, Santos, Daniela Filipa, Onofre de Carvalho, Ana Paula, Pereira, Ana Catarina, Izquierdo-Álvarez, Alicia, Ramos, Elena, Morato-López, Esperanza, Marina, Anabel, Martínez-Ruiz, Antonio, Araújo, Inés María
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/236867
Acesso em linha:http://hdl.handle.net/10261/236867
Access Level:acceso abierto
Palavra-chave:Nitric oxide
S-nitrosylation
Neural stem cells
Neurogenesis
Seizures
Hippocampus
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spelling Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomicsSantos, Ana IsabelLourenço, Ana SofíaSimão, SóniaMarques da Silva, DorindaSantos, Daniela FilipaOnofre de Carvalho, Ana PaulaPereira, Ana CatarinaIzquierdo-Álvarez, AliciaRamos, ElenaMorato-López, EsperanzaMarina, AnabelMartínez-Ruiz, AntonioAraújo, Inés MaríaNitric oxideS-nitrosylationNeural stem cellsNeurogenesisSeizuresHippocampusNitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S-nitrosylation. This post-translational modification consists in the formation of a nitrosothiol group (R–SNO) in cysteine residues, which can promote formation of other oxidative modifications in those cysteine residues. S-nitrosylation can regulate many physiological processes, including neuronal plasticity and neurogenesis. In this work, we aimed to identify S-nitrosylation targets of NO that could participate in neurogenesis. In NSC, we identified a group of proteins oxidatively modified using complementary techniques of thiol redox proteomics. S-nitrosylation of some of these proteins was confirmed and validated in a seizure mouse model of hippocampal injury and in cultured hippocampal stem cells. The identified S-nitrosylated proteins are involved in the ERK/MAPK pathway and may be important targets of NO to enhance the proliferation of NSC.Spanish Government (grants PS09/00101, PI12/00875 and PI15/00107 from ISCIII and RTI2018-094203-B-I00 from AEI; co-financed by FEDER/ERDF) and by the Spanish-Portuguese Integrated Action grant PRI-AIBPT-2011-1015/E-10/12.Instituto de Salud Carlos IIIConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/236867reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.redox.2020.101457Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2368672026-05-22T06:33:51Z
dc.title.none.fl_str_mv Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics
title Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics
spellingShingle Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics
Santos, Ana Isabel
Nitric oxide
S-nitrosylation
Neural stem cells
Neurogenesis
Seizures
Hippocampus
title_short Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics
title_full Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics
title_fullStr Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics
title_full_unstemmed Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics
title_sort Identification of new targets of S-nitrosylation in neural stem cells by thiol redox proteomics
dc.creator.none.fl_str_mv Santos, Ana Isabel
Lourenço, Ana Sofía
Simão, Sónia
Marques da Silva, Dorinda
Santos, Daniela Filipa
Onofre de Carvalho, Ana Paula
Pereira, Ana Catarina
Izquierdo-Álvarez, Alicia
Ramos, Elena
Morato-López, Esperanza
Marina, Anabel
Martínez-Ruiz, Antonio
Araújo, Inés María
author Santos, Ana Isabel
author_facet Santos, Ana Isabel
Lourenço, Ana Sofía
Simão, Sónia
Marques da Silva, Dorinda
Santos, Daniela Filipa
Onofre de Carvalho, Ana Paula
Pereira, Ana Catarina
Izquierdo-Álvarez, Alicia
Ramos, Elena
Morato-López, Esperanza
Marina, Anabel
Martínez-Ruiz, Antonio
Araújo, Inés María
author_role author
author2 Lourenço, Ana Sofía
Simão, Sónia
Marques da Silva, Dorinda
Santos, Daniela Filipa
Onofre de Carvalho, Ana Paula
Pereira, Ana Catarina
Izquierdo-Álvarez, Alicia
Ramos, Elena
Morato-López, Esperanza
Marina, Anabel
Martínez-Ruiz, Antonio
Araújo, Inés María
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Nitric oxide
S-nitrosylation
Neural stem cells
Neurogenesis
Seizures
Hippocampus
topic Nitric oxide
S-nitrosylation
Neural stem cells
Neurogenesis
Seizures
Hippocampus
description Nitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation of neural stem cells (NSC), and is essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One of the mechanisms underlying non-classical NO cell signaling is protein S-nitrosylation. This post-translational modification consists in the formation of a nitrosothiol group (R–SNO) in cysteine residues, which can promote formation of other oxidative modifications in those cysteine residues. S-nitrosylation can regulate many physiological processes, including neuronal plasticity and neurogenesis. In this work, we aimed to identify S-nitrosylation targets of NO that could participate in neurogenesis. In NSC, we identified a group of proteins oxidatively modified using complementary techniques of thiol redox proteomics. S-nitrosylation of some of these proteins was confirmed and validated in a seizure mouse model of hippocampal injury and in cultured hippocampal stem cells. The identified S-nitrosylated proteins are involved in the ERK/MAPK pathway and may be important targets of NO to enhance the proliferation of NSC.
publishDate 2020
dc.date.none.fl_str_mv 2020
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/236867
url http://hdl.handle.net/10261/236867
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.redox.2020.101457
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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