Sistemas combinados de ácido hialurónico y polímeros acrílicos como biomateriales para ingeniería tisular
Hyaluronic acid, HA, and poly (ethyl acrylate), PEA, are two polymers widely used in biomedical applications, especially in tissue engineering, because of their excellent biocompatible and bioactive properties. HA is a highly hydrophilic biopolymer and the PEA, on the other hand, is hydrophobic. In...
| Autor: | |
|---|---|
| Tipo de recurso: | tesis doctoral |
| Fecha de publicación: | 2017 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | español |
| OAI Identifier: | oai:riunet.upv.es:10251/90649 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/90649 |
| Access Level: | acceso abierto |
| Palabra clave: | Ácido hialurónico Polímeros acrílicos Sistemas combinados Biomateriales Biocompatibilidad Ingeniería tisular Biotecnología |
| Sumario: | Hyaluronic acid, HA, and poly (ethyl acrylate), PEA, are two polymers widely used in biomedical applications, especially in tissue engineering, because of their excellent biocompatible and bioactive properties. HA is a highly hydrophilic biopolymer and the PEA, on the other hand, is hydrophobic. In addition, each one of them has certain shortcomings that limit the potential of its application, so that being able to combine them in a viable way in one biomaterial is of great interest and in turn is a very promising and attractive challenge for the development of new biomaterials. In this thesis, several HA-PEA combined systems are developed, making the two phases compatible in non-crosslinked state, using formic acid, FA, as a common solvent for the two polymers. The effect of formic acid on the materials is previously evaluated, ruling out chemical modification, degradation or generation of cytotoxicity. The combined systems have been developed with different geometries and architectures, in the form of two-dimensional films or blends, spun membranes and three-dimensional porous scaffolds, using solvent casting, electrospinning and freeze-extraction techniques. The physicochemical properties reveal a certain mutual reinforcement produced by both phases, and the biological characterization highlights the potential of the system as biomaterial. Additionally, other systems are made by copolymerizing PEA with ethyl 2-carboxy acrylate, CEA, in either cross-linked or non-crosslinked state. As they are new products, we proceed to their complete physico-chemical and biological characterization, confirming their aptitude as biomaterials. Also studied is the the possibility of combining these copolymers with hyaluronic acid to obtain materials with better properties than those already achieved in the HA-PEA systems. |
|---|