An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus

Airborne animal viral pathogens can rapidly spread and become a global threat, resulting in substantial socioeconomic and health consequences. To prevent and control potential epidemic outbreaks, accurate, fast, and affordable point-of-care (POC) tests are essential. As a proof-of-concept, we have d...

Descripción completa

Detalles Bibliográficos
Autores: Cea Callejo, Pablo, Arca Lafuente, Sonia, Gómez-Lucía Duato, María Esperanza, Domenech Gómez, Ana María, Biarnés, Mar, Blanco, Ángela, Benítez Rico, Laura, Madrid González, Ricardo
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/107361
Acceso en línea:https://hdl.handle.net/20.500.14352/107361
Access Level:acceso abierto
Palabra clave:636.09
Molecular detection
Reverse transcription loop mediated isothermal amplifcation (RT-LAMP)
Nanoprobes
Avian metapneumovirus (aMPV)
Point of Care (POC) test
Veterinaria
3109 Ciencias Veterinarias
id ES_6ad42e2eb8e8e0ff732add72133ea790
oai_identifier_str oai:docta.ucm.es:20.500.14352/107361
network_acronym_str ES
network_name_str España
repository_id_str
spelling An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirusCea Callejo, PabloArca Lafuente, SoniaGómez-Lucía Duato, María EsperanzaDomenech Gómez, Ana MaríaBiarnés, MarBlanco, ÁngelaBenítez Rico, LauraMadrid González, Ricardo636.09Molecular detectionReverse transcription loop mediated isothermal amplifcation (RT-LAMP)NanoprobesAvian metapneumovirus (aMPV)Point of Care (POC) testVeterinaria3109 Ciencias VeterinariasAirborne animal viral pathogens can rapidly spread and become a global threat, resulting in substantial socioeconomic and health consequences. To prevent and control potential epidemic outbreaks, accurate, fast, and affordable point-of-care (POC) tests are essential. As a proof-of-concept, we have developed a molecular system based on the loop-mediated isothermal amplification (LAMP) technique for avian metapneumovirus (aMPV) detection, an airborne communicable agent mainly infecting turkeys and chickens. For this purpose, a colorimetric system was obtained by coupling the LAMP technique with specific DNA-functionalized AuNPs (gold nanoparticles). The system was validated using 50 different samples (pharyngeal swabs and tracheal tissue) collected from aMPV-infected and non-infected chickens and turkeys. Viral detection can be achieved in about 60 min with the naked eye, with 100% specificity and 87.88% sensitivity for aMPV. In summary, this novel molecular detection system allows suitable virus testing in the field, with accuracy and limit of detection (LOD) values highly close to qRT-PCR-based diagnosis. Furthermore, this system can be easily scalable to a platform for the detection of other viruses, addressing the current gap in the availability of POC tests for viral detection in poultry farming.SpringerUniversidad Complutense de Madrid20242024-07-1020242024-07-10journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/107361reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/1073612026-06-02T12:44:21Z
dc.title.none.fl_str_mv An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
title An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
spellingShingle An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
Cea Callejo, Pablo
636.09
Molecular detection
Reverse transcription loop mediated isothermal amplifcation (RT-LAMP)
Nanoprobes
Avian metapneumovirus (aMPV)
Point of Care (POC) test
Veterinaria
3109 Ciencias Veterinarias
title_short An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
title_full An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
title_fullStr An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
title_full_unstemmed An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
title_sort An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus
dc.creator.none.fl_str_mv Cea Callejo, Pablo
Arca Lafuente, Sonia
Gómez-Lucía Duato, María Esperanza
Domenech Gómez, Ana María
Biarnés, Mar
Blanco, Ángela
Benítez Rico, Laura
Madrid González, Ricardo
author Cea Callejo, Pablo
author_facet Cea Callejo, Pablo
Arca Lafuente, Sonia
Gómez-Lucía Duato, María Esperanza
Domenech Gómez, Ana María
Biarnés, Mar
Blanco, Ángela
Benítez Rico, Laura
Madrid González, Ricardo
author_role author
author2 Arca Lafuente, Sonia
Gómez-Lucía Duato, María Esperanza
Domenech Gómez, Ana María
Biarnés, Mar
Blanco, Ángela
Benítez Rico, Laura
Madrid González, Ricardo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 636.09
Molecular detection
Reverse transcription loop mediated isothermal amplifcation (RT-LAMP)
Nanoprobes
Avian metapneumovirus (aMPV)
Point of Care (POC) test
Veterinaria
3109 Ciencias Veterinarias
topic 636.09
Molecular detection
Reverse transcription loop mediated isothermal amplifcation (RT-LAMP)
Nanoprobes
Avian metapneumovirus (aMPV)
Point of Care (POC) test
Veterinaria
3109 Ciencias Veterinarias
description Airborne animal viral pathogens can rapidly spread and become a global threat, resulting in substantial socioeconomic and health consequences. To prevent and control potential epidemic outbreaks, accurate, fast, and affordable point-of-care (POC) tests are essential. As a proof-of-concept, we have developed a molecular system based on the loop-mediated isothermal amplification (LAMP) technique for avian metapneumovirus (aMPV) detection, an airborne communicable agent mainly infecting turkeys and chickens. For this purpose, a colorimetric system was obtained by coupling the LAMP technique with specific DNA-functionalized AuNPs (gold nanoparticles). The system was validated using 50 different samples (pharyngeal swabs and tracheal tissue) collected from aMPV-infected and non-infected chickens and turkeys. Viral detection can be achieved in about 60 min with the naked eye, with 100% specificity and 87.88% sensitivity for aMPV. In summary, this novel molecular detection system allows suitable virus testing in the field, with accuracy and limit of detection (LOD) values highly close to qRT-PCR-based diagnosis. Furthermore, this system can be easily scalable to a platform for the detection of other viruses, addressing the current gap in the availability of POC tests for viral detection in poultry farming.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-07-10
2024
2024-07-10
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/107361
url https://hdl.handle.net/20.500.14352/107361
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410140743008257
score 15.811543

Similares en LA Referencia