Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplification
The aim of this work is the design and 3D printing of a new electrochemical sensor for the detection of Listeria monocytogenes based on loop mediated isothermal amplification (LAMP). The food related diseases involve a serious health issue all over the world. Listeria monocytogenes is one of the maj...
| Autores: | , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2023 |
| País: | España |
| Institución: | Universidad del País Vasco |
| Repositorio: | Addi. Archivo Digital para la Docencia y la Investigación |
| OAI Identifier: | oai:addi.ehu.eus:10810/61091 |
| Acceso en línea: | http://hdl.handle.net/10810/61091 |
| Access Level: | acceso abierto |
| Palabra clave: | loop -mediated isothermal AMPlification (LAMP) Listeria monocytogenes electrochemical detection biosensor 3D printing numerical analysis |
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Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplificationRivas Macho, AneEletxigerra, UnaiDíez Ahedo, RuthMerino Álvarez, SantosSanjuan, AnttonBou-Ali, M. MounirRuiz Rubio, LeireDel Campo, JavierVilas Vilela, José LuisGoñi de Cerio, FelipeOlabarria, Garbiñeloop -mediated isothermal AMPlification(LAMP)Listeria monocytogeneselectrochemical detectionbiosensor3D printingnumerical analysisThe aim of this work is the design and 3D printing of a new electrochemical sensor for the detection of Listeria monocytogenes based on loop mediated isothermal amplification (LAMP). The food related diseases involve a serious health issue all over the world. Listeria monocytogenes is one of the major problems of contaminated food, this pathogen causes a disease called listeriosis with a high rate of hospitalization and mortality. Having a fast, sensitive and specific detection method for food quality control is a must in the food industry to avoid the presence of this pathogen in the food chain (raw materials, facilities and products). A point-of-care biosensor based in LAMP and electrochemical detection is one of the best options to detect the bacteria on site and in a very short period of time. With the numerical analysis of different geometries and flow rates during sample injection in order to avoid bubbles, an optimized design of the microfluidic biosensor chamber was selected for 3D-printing and experimental analysis. For the electrochemical detection, a novel custom gold concentric-3-electrode consisting in a working electrode, reference electrode and a counter electrode was designed and placed in the bottom of the chamber. The LAMP reaction was optimized specifically for a primers set with a limit of detection of 1.25 pg of genomic DNA per reaction and 100% specific for detecting all 12 Listeria monocytogenes serotypes and no other Listeria species or food-related bacteria. The methylene blue redox-active molecule was tested as the electrochemical transducer and shown to be compatible with the LAMP reaction and very clearly distinguished negative from positive food samples when the reaction is measured at the end-point inside the biosensor.Garbiñe Olabarria as supported by Ekonomiaren Garapen eta Lehiakortasun Saila, Eusko Jaurlaritza [KK-2021/00082]. M. Mounir Bou-Ali was supported by Eusko Jaurlaritza [Research Group Program, IT1505-22].Elsevier202320232023info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/61091reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://www.sciencedirect.com/science/article/pii/S2405844022039251?via%3Dihubinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/3.0/es/© 2023 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).Atribución-NoComercial-SinDerivadas 3.0 Españaoai:addi.ehu.eus:10810/610912026-06-18T09:23:17Z |
| dc.title.none.fl_str_mv |
Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplification |
| title |
Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplification |
| spellingShingle |
Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplification Rivas Macho, Ane loop -mediated isothermal AMPlification (LAMP) Listeria monocytogenes electrochemical detection biosensor 3D printing numerical analysis |
| title_short |
Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplification |
| title_full |
Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplification |
| title_fullStr |
Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplification |
| title_full_unstemmed |
Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplification |
| title_sort |
Design and 3D printing of an electrochemical sensor for Listeria monocytogenes detection based on loop mediated isothermal amplification |
| dc.creator.none.fl_str_mv |
Rivas Macho, Ane Eletxigerra, Unai Díez Ahedo, Ruth Merino Álvarez, Santos Sanjuan, Antton Bou-Ali, M. Mounir Ruiz Rubio, Leire Del Campo, Javier Vilas Vilela, José Luis Goñi de Cerio, Felipe Olabarria, Garbiñe |
| author |
Rivas Macho, Ane |
| author_facet |
Rivas Macho, Ane Eletxigerra, Unai Díez Ahedo, Ruth Merino Álvarez, Santos Sanjuan, Antton Bou-Ali, M. Mounir Ruiz Rubio, Leire Del Campo, Javier Vilas Vilela, José Luis Goñi de Cerio, Felipe Olabarria, Garbiñe |
| author_role |
author |
| author2 |
Eletxigerra, Unai Díez Ahedo, Ruth Merino Álvarez, Santos Sanjuan, Antton Bou-Ali, M. Mounir Ruiz Rubio, Leire Del Campo, Javier Vilas Vilela, José Luis Goñi de Cerio, Felipe Olabarria, Garbiñe |
| author2_role |
author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
loop -mediated isothermal AMPlification (LAMP) Listeria monocytogenes electrochemical detection biosensor 3D printing numerical analysis |
| topic |
loop -mediated isothermal AMPlification (LAMP) Listeria monocytogenes electrochemical detection biosensor 3D printing numerical analysis |
| description |
The aim of this work is the design and 3D printing of a new electrochemical sensor for the detection of Listeria monocytogenes based on loop mediated isothermal amplification (LAMP). The food related diseases involve a serious health issue all over the world. Listeria monocytogenes is one of the major problems of contaminated food, this pathogen causes a disease called listeriosis with a high rate of hospitalization and mortality. Having a fast, sensitive and specific detection method for food quality control is a must in the food industry to avoid the presence of this pathogen in the food chain (raw materials, facilities and products). A point-of-care biosensor based in LAMP and electrochemical detection is one of the best options to detect the bacteria on site and in a very short period of time. With the numerical analysis of different geometries and flow rates during sample injection in order to avoid bubbles, an optimized design of the microfluidic biosensor chamber was selected for 3D-printing and experimental analysis. For the electrochemical detection, a novel custom gold concentric-3-electrode consisting in a working electrode, reference electrode and a counter electrode was designed and placed in the bottom of the chamber. The LAMP reaction was optimized specifically for a primers set with a limit of detection of 1.25 pg of genomic DNA per reaction and 100% specific for detecting all 12 Listeria monocytogenes serotypes and no other Listeria species or food-related bacteria. The methylene blue redox-active molecule was tested as the electrochemical transducer and shown to be compatible with the LAMP reaction and very clearly distinguished negative from positive food samples when the reaction is measured at the end-point inside the biosensor. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023 2023 2023 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10810/61091 |
| url |
http://hdl.handle.net/10810/61091 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
https://www.sciencedirect.com/science/article/pii/S2405844022039251?via%3Dihub |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Atribución-NoComercial-SinDerivadas 3.0 España |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-nd/3.0/es/ Atribución-NoComercial-SinDerivadas 3.0 España |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:Addi. Archivo Digital para la Docencia y la Investigación instname:Universidad del País Vasco |
| instname_str |
Universidad del País Vasco |
| reponame_str |
Addi. Archivo Digital para la Docencia y la Investigación |
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Addi. Archivo Digital para la Docencia y la Investigación |
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15,300724 |