CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators

In the context of obesity, strong evidences support a distinctive pathological contribution of adipose tissue depending on its anatomical site of accumulation. Therefore, subcutaneous adipose tissue (SAT) has been lately considered metabolically benign compared to visceral fat (VAT), whose location...

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Autores: Roca Rivada, Arturo, Bravo López, Susana Belén, Pérez Sotelo, Diego, Alonso Lorenzo, Jana, Castro Pais, Ana Isabel, Baamonde de la Torre, Ivan, Baltar Boileve, Javier, Casanueva Freijo, Felipe, Pardo Pumar, María Isabel
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Servizo Galego de Saúde (SERGAS)
Repositorio:RUNA. Repositorio da Consellería de Sanidade e Sergas
OAI Identifier:oai:runa.sergas.gal:20.500.11940/8341
Acceso en línea:http://hdl.handle.net/20.500.11940/8341
Access Level:acceso abierto
Palabra clave:Adipose Tissue
Amino Acids
Extracellular Matrix
Humans
Inflammation
Inflammation Mediators
Insulin Resistance
Intra-Abdominal Fat
Isotope Labeling
Obesity
Proteomics
Subcutaneous Fat
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spelling CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation MediatorsRoca Rivada, ArturoBravo López, Susana BelénPérez Sotelo, DiegoAlonso Lorenzo, JanaCastro Pais, Ana IsabelBaamonde de la Torre, IvanBaltar Boileve, JavierCasanueva Freijo, FelipePardo Pumar, María IsabelAdipose TissueAmino AcidsExtracellular MatrixHumansInflammationInflammation MediatorsInsulin ResistanceIntra-Abdominal FatIsotope LabelingObesityProteomicsSubcutaneous FatIn the context of obesity, strong evidences support a distinctive pathological contribution of adipose tissue depending on its anatomical site of accumulation. Therefore, subcutaneous adipose tissue (SAT) has been lately considered metabolically benign compared to visceral fat (VAT), whose location is associated to the risk of developing cardiovascular disease, insulin resistance, and other associated comorbidities. Under the above situation, the chronic local inflammation that characterizes obese adipose tissue, has acquired a major role on the pathogenesis of obesity. In this work, we have analyzed for the first time human obese VAT and SAT secretomes using an improved quantitative proteomic approach for the study of tissue secretomes, Comparison of Isotope-Labeled Amino acid Incorporation Rates (CILAIR). The use of double isotope-labeling-CILAIR approach to analyze VAT and SAT secretomes allowed the identification of location-specific secreted proteins and its differential secretion. Additionally to the very high percentage of identified proteins previously implicated in obesity or in its comorbidities, this approach was revealed as a useful tool for the study of the obese adipose tissue microenvironment including extracellular matrix (ECM) remodeling and inflammatory status. The results herein presented reinforce the fact that VAT and SAT depots have distinct features and contribute differentially to metabolic disease.2015info:eu-repo/semantics/articlehttp://hdl.handle.net/20.500.11940/8341reponame:RUNA. Repositorio da Consellería de Sanidade e Sergasinstname:Servizo Galego de Saúde (SERGAS)Ingléshttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:runa.sergas.gal:20.500.11940/83412026-06-12T08:40:47Z
dc.title.none.fl_str_mv CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators
title CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators
spellingShingle CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators
Roca Rivada, Arturo
Adipose Tissue
Amino Acids
Extracellular Matrix
Humans
Inflammation
Inflammation Mediators
Insulin Resistance
Intra-Abdominal Fat
Isotope Labeling
Obesity
Proteomics
Subcutaneous Fat
title_short CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators
title_full CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators
title_fullStr CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators
title_full_unstemmed CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators
title_sort CILAIR-Based Secretome Analysis of Obese Visceral and Subcutaneous Adipose Tissues Reveals Distinctive ECM Remodeling and Inflammation Mediators
dc.creator.none.fl_str_mv Roca Rivada, Arturo
Bravo López, Susana Belén
Pérez Sotelo, Diego
Alonso Lorenzo, Jana
Castro Pais, Ana Isabel
Baamonde de la Torre, Ivan
Baltar Boileve, Javier
Casanueva Freijo, Felipe
Pardo Pumar, María Isabel
author Roca Rivada, Arturo
author_facet Roca Rivada, Arturo
Bravo López, Susana Belén
Pérez Sotelo, Diego
Alonso Lorenzo, Jana
Castro Pais, Ana Isabel
Baamonde de la Torre, Ivan
Baltar Boileve, Javier
Casanueva Freijo, Felipe
Pardo Pumar, María Isabel
author_role author
author2 Bravo López, Susana Belén
Pérez Sotelo, Diego
Alonso Lorenzo, Jana
Castro Pais, Ana Isabel
Baamonde de la Torre, Ivan
Baltar Boileve, Javier
Casanueva Freijo, Felipe
Pardo Pumar, María Isabel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adipose Tissue
Amino Acids
Extracellular Matrix
Humans
Inflammation
Inflammation Mediators
Insulin Resistance
Intra-Abdominal Fat
Isotope Labeling
Obesity
Proteomics
Subcutaneous Fat
topic Adipose Tissue
Amino Acids
Extracellular Matrix
Humans
Inflammation
Inflammation Mediators
Insulin Resistance
Intra-Abdominal Fat
Isotope Labeling
Obesity
Proteomics
Subcutaneous Fat
description In the context of obesity, strong evidences support a distinctive pathological contribution of adipose tissue depending on its anatomical site of accumulation. Therefore, subcutaneous adipose tissue (SAT) has been lately considered metabolically benign compared to visceral fat (VAT), whose location is associated to the risk of developing cardiovascular disease, insulin resistance, and other associated comorbidities. Under the above situation, the chronic local inflammation that characterizes obese adipose tissue, has acquired a major role on the pathogenesis of obesity. In this work, we have analyzed for the first time human obese VAT and SAT secretomes using an improved quantitative proteomic approach for the study of tissue secretomes, Comparison of Isotope-Labeled Amino acid Incorporation Rates (CILAIR). The use of double isotope-labeling-CILAIR approach to analyze VAT and SAT secretomes allowed the identification of location-specific secreted proteins and its differential secretion. Additionally to the very high percentage of identified proteins previously implicated in obesity or in its comorbidities, this approach was revealed as a useful tool for the study of the obese adipose tissue microenvironment including extracellular matrix (ECM) remodeling and inflammatory status. The results herein presented reinforce the fact that VAT and SAT depots have distinct features and contribute differentially to metabolic disease.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.11940/8341
url http://hdl.handle.net/20.500.11940/8341
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:RUNA. Repositorio da Consellería de Sanidade e Sergas
instname:Servizo Galego de Saúde (SERGAS)
instname_str Servizo Galego de Saúde (SERGAS)
reponame_str RUNA. Repositorio da Consellería de Sanidade e Sergas
collection RUNA. Repositorio da Consellería de Sanidade e Sergas
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410131575308288
score 15.300719

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