Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)

© 2021 by the authors.

Detalhes bibliográficos
Autores: Picard-Sánchez, Amparo, Piazzon de Haro, María Carla, Estensoro, Itziar, Pozo, R. del, Ahmed, Nahla Hossameldin, Palenzuela, Oswaldo, Sitjà-Bobadilla, Ariadna
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/267044
Acesso em linha:http://hdl.handle.net/10261/267044
Access Level:Acceso aberto
Palavra-chave:Sparus aurata
Experimental transmission
Enterospora nucleophila
Teleostei
Microsporidia
Aquaculture
Temperatures
Immunosuppression
Histopathology
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spelling Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)Picard-Sánchez, AmparoPiazzon de Haro, María CarlaEstensoro, ItziarPozo, R. delAhmed, Nahla HossameldinPalenzuela, OswaldoSitjà-Bobadilla, AriadnaSparus aurataExperimental transmissionEnterospora nucleophilaTeleosteiMicrosporidiaAquacultureTemperaturesImmunosuppressionHistopathology© 2021 by the authors.Enterospora nucleophila is a microsporidian enteroparasite that infects mainly the intestine of gilthead sea bream (Sparus aurata), leading to an emaciative syndrome. Thus far, the only available information about this infection comes from natural outbreaks in farmed fish. The aim of the present study was to determine whether E. nucleophila could be transmitted horizontally using naturally infected fish as donors, and to establish an experimental in vivo procedure to study this host–parasite model without depending on natural infections. Naïve fish were exposed to the infection by cohabitation, effluent, or intubated either orally or anally with intestinal scrapings of donor fish in four different trials. We succeeded in detecting parasite in naïve fish in all the challenges, but the infection level and the disease signs were always milder than in donor fish. The parasite was found in peripheral blood of naïve fish at 4 weeks post-challenge (wpc) in oral and effluent routes, and up to 12 wpc in the anal transmission trial. Molecular diagnosis detected E. nucleophila in other organs besides intestine, such as gills, liver, stomach or heart, although the intensity was not as high as in the target tissue. The infection tended to disappear through time in all the challenge routes assayed, except in the anal infection route.This work has been carried out with financial support from the European Union and the Spanish MINECO under grant projects ParaFishControl (H2020-634429) and AGL2013-R-48560-C2-2-R, respectively. A.P.-S. was contracted under ParaFishControl project. This publication reflects only the authors’ view, and the European Commission cannot be held responsible for any use which may be made of the information contained herein. M.C.P. was funded by a Ramón y Cajal Postdoctoral Research Fellowship (RYC2018-024049-I/AEI/10.13039/501100011033 and ACOND/2020 Generalitat Valenciana), and R.P. was contracted under the PTA-Program from the Spanish Ministry of Science, Innovation and Universities (PTA2018-015315-I). Both contracts have been co-funded by the European Social Fund (ESF).We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI).Multidisciplinary Digital Publishing InstituteEuropean CommissionMinisterio de Economía y Competitividad (España)Agencia Estatal de Investigación (España)Generalitat ValencianaCSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220212022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/267044reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/634429info:eu-repo/grantAgreement/MINECO//AGL2013-48560-C2-2-Rinfo:eu-repo/grantAgreement///info:eu-repo/grantAgreement///http://dx.doi.org/10.3390/ani11020362Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2670442026-05-22T06:33:51Z
dc.title.none.fl_str_mv Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)
title Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)
spellingShingle Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)
Picard-Sánchez, Amparo
Sparus aurata
Experimental transmission
Enterospora nucleophila
Teleostei
Microsporidia
Aquaculture
Temperatures
Immunosuppression
Histopathology
title_short Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)
title_full Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)
title_fullStr Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)
title_full_unstemmed Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)
title_sort Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)
dc.creator.none.fl_str_mv Picard-Sánchez, Amparo
Piazzon de Haro, María Carla
Estensoro, Itziar
Pozo, R. del
Ahmed, Nahla Hossameldin
Palenzuela, Oswaldo
Sitjà-Bobadilla, Ariadna
author Picard-Sánchez, Amparo
author_facet Picard-Sánchez, Amparo
Piazzon de Haro, María Carla
Estensoro, Itziar
Pozo, R. del
Ahmed, Nahla Hossameldin
Palenzuela, Oswaldo
Sitjà-Bobadilla, Ariadna
author_role author
author2 Piazzon de Haro, María Carla
Estensoro, Itziar
Pozo, R. del
Ahmed, Nahla Hossameldin
Palenzuela, Oswaldo
Sitjà-Bobadilla, Ariadna
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Economía y Competitividad (España)
Agencia Estatal de Investigación (España)
Generalitat Valenciana
CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Sparus aurata
Experimental transmission
Enterospora nucleophila
Teleostei
Microsporidia
Aquaculture
Temperatures
Immunosuppression
Histopathology
topic Sparus aurata
Experimental transmission
Enterospora nucleophila
Teleostei
Microsporidia
Aquaculture
Temperatures
Immunosuppression
Histopathology
description © 2021 by the authors.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/267044
url http://hdl.handle.net/10261/267044
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/634429
info:eu-repo/grantAgreement/MINECO//AGL2013-48560-C2-2-R
info:eu-repo/grantAgreement///
info:eu-repo/grantAgreement///
http://dx.doi.org/10.3390/ani11020362
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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