Diversity of the cell-wall associated genomic island of the archaeon Haloquadratum walsbyi

Background: Haloquadratum walsbyi represents up to 80 % of cells in NaCl-saturated brines worldwide, but is notoriously difficult to maintain under laboratory conditions. In order to establish the extent of genetic diversity in a natural population of this microbe, we screened a H. walsbyi enriched...

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Detalles Bibliográficos
Autores: Martín Cuadrado, Ana Belén, Pašić, Lejla, Rodríguez Valera, Francisco
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universidad Miguel Hernández de Elche
Repositorio:REDIUMH. Depósito Digital de la UMH
OAI Identifier:oai:dspace.umh.es:11000/4811
Acceso en línea:http://hdl.handle.net/11000/4811
Access Level:acceso abierto
Palabra clave:Cell surface glycoproteins
S-layer
Cell-wall
Environmental fosmid library
Haloquadratum walsbyi
579 - Microbiología
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spelling Diversity of the cell-wall associated genomic island of the archaeon Haloquadratum walsbyiMartín Cuadrado, Ana BelénPašić, LejlaRodríguez Valera, FranciscoCell surface glycoproteinsS-layerCell-wallEnvironmental fosmid libraryHaloquadratum walsbyi579 - MicrobiologíaBackground: Haloquadratum walsbyi represents up to 80 % of cells in NaCl-saturated brines worldwide, but is notoriously difficult to maintain under laboratory conditions. In order to establish the extent of genetic diversity in a natural population of this microbe, we screened a H. walsbyi enriched metagenomic fosmid library and recovered seven novel version of its cell-wall associated genomic island. The fosmid inserts were sequenced and analysed. Results: The novel cell-wall associated islands delineated two major clades within H. walsbyi. The islands predominantly contained genes putatively involved in biosynthesis of surface layer, genes encoding cell surface glycoproteins and genes involved in envelope formation. We further found that these genes are maintained in the population and that the diversity of this region arises through homologous recombination but also through the action of mobile genetic elements, including viruses. Conclusions: The population of H. walsbyi in the studied saltern brine is composed of numerous clonal lineages that differ in surface structures including the cell wall. This type of variation probably reflects a number of mechanisms that minimize the infection rate of predating virusesAll authors were supported by project MICROGEN (Programa CONSOLIDER-INGENIO 2010 CSD2009-00006) from the Spanish Ministerio de Ciencia e Innovación.FR-V and AMBC received support from MEDIMAX BFPU2013- 48007-P from the Spanish Ministerio de Economía y CompetitividadMaCuMBA Project 311975 of the European Commission FP7ACOMP/2014/024, AORG 2014/032 and PROMETEO II/2014/012. LP received support from Ministry for School and Sports of the Republic of Slovenia under Slovenian Research Agency program P1-0198.Departamentos de la UMH::Producción Vegetal y Microbiología2018201820152018info:eu-repo/semantics/articleapplication/pdf12application/pdfhttp://hdl.handle.net/11000/4811reponame:REDIUMH. Depósito Digital de la UMHinstname:Universidad Miguel Hernández de ElcheIngléshttps://doi.org/10.1186/s12864-015-1794-8info:eu-repo/semantics/openAccessoai:dspace.umh.es:11000/48112026-05-27T13:36:21Z
dc.title.none.fl_str_mv Diversity of the cell-wall associated genomic island of the archaeon Haloquadratum walsbyi
title Diversity of the cell-wall associated genomic island of the archaeon Haloquadratum walsbyi
spellingShingle Diversity of the cell-wall associated genomic island of the archaeon Haloquadratum walsbyi
Martín Cuadrado, Ana Belén
Cell surface glycoproteins
S-layer
Cell-wall
Environmental fosmid library
Haloquadratum walsbyi
579 - Microbiología
title_short Diversity of the cell-wall associated genomic island of the archaeon Haloquadratum walsbyi
title_full Diversity of the cell-wall associated genomic island of the archaeon Haloquadratum walsbyi
title_fullStr Diversity of the cell-wall associated genomic island of the archaeon Haloquadratum walsbyi
title_full_unstemmed Diversity of the cell-wall associated genomic island of the archaeon Haloquadratum walsbyi
title_sort Diversity of the cell-wall associated genomic island of the archaeon Haloquadratum walsbyi
dc.creator.none.fl_str_mv Martín Cuadrado, Ana Belén
Pašić, Lejla
Rodríguez Valera, Francisco
author Martín Cuadrado, Ana Belén
author_facet Martín Cuadrado, Ana Belén
Pašić, Lejla
Rodríguez Valera, Francisco
author_role author
author2 Pašić, Lejla
Rodríguez Valera, Francisco
author2_role author
author
dc.contributor.none.fl_str_mv Departamentos de la UMH::Producción Vegetal y Microbiología
dc.subject.none.fl_str_mv Cell surface glycoproteins
S-layer
Cell-wall
Environmental fosmid library
Haloquadratum walsbyi
579 - Microbiología
topic Cell surface glycoproteins
S-layer
Cell-wall
Environmental fosmid library
Haloquadratum walsbyi
579 - Microbiología
description Background: Haloquadratum walsbyi represents up to 80 % of cells in NaCl-saturated brines worldwide, but is notoriously difficult to maintain under laboratory conditions. In order to establish the extent of genetic diversity in a natural population of this microbe, we screened a H. walsbyi enriched metagenomic fosmid library and recovered seven novel version of its cell-wall associated genomic island. The fosmid inserts were sequenced and analysed. Results: The novel cell-wall associated islands delineated two major clades within H. walsbyi. The islands predominantly contained genes putatively involved in biosynthesis of surface layer, genes encoding cell surface glycoproteins and genes involved in envelope formation. We further found that these genes are maintained in the population and that the diversity of this region arises through homologous recombination but also through the action of mobile genetic elements, including viruses. Conclusions: The population of H. walsbyi in the studied saltern brine is composed of numerous clonal lineages that differ in surface structures including the cell wall. This type of variation probably reflects a number of mechanisms that minimize the infection rate of predating viruses
publishDate 2015
dc.date.none.fl_str_mv 2015
2018
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/11000/4811
url http://hdl.handle.net/11000/4811
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1186/s12864-015-1794-8
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
12
application/pdf
dc.source.none.fl_str_mv reponame:REDIUMH. Depósito Digital de la UMH
instname:Universidad Miguel Hernández de Elche
instname_str Universidad Miguel Hernández de Elche
reponame_str REDIUMH. Depósito Digital de la UMH
collection REDIUMH. Depósito Digital de la UMH
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301603

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