Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography–ultraviolet detection analysis

Methods developed for porphyrin analysis have low recoveries and/or poor precision for the less polar protoporphyrin IX. We describe a simple method of analysis of porphyrins and biliverdin in bile and excreta of birds based on extraction with HCl 3N: acetonitrile and HPLC/UV analyses. Recoveries we...

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Detalles Bibliográficos
Autores: Mateo, Rafael, Castells, Gloria, Green, Andy J., Godoy, Carlo, Cristòfal, Carles
Tipo de recurso: artículo
Fecha de publicación:2004
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/42926
Acceso en línea:http://hdl.handle.net/10261/42926
Access Level:acceso abierto
Palabra clave:Porphyrins
Biliverdin
Lead poisoning
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spelling Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography–ultraviolet detection analysisMateo, RafaelCastells, GloriaGreen, Andy J.Godoy, CarloCristòfal, CarlesPorphyrinsBiliverdinLead poisoningMethods developed for porphyrin analysis have low recoveries and/or poor precision for the less polar protoporphyrin IX. We describe a simple method of analysis of porphyrins and biliverdin in bile and excreta of birds based on extraction with HCl 3N: acetonitrile and HPLC/UV analyses. Recoveries were good for protoporphyrin IX and other porphyrins (>79%). Applications of this method showed that porphyrins and biliverdin in birds excreta are mainly of biliary-fecal origin rather than urinary origin. Biliverdin and protoporphyrin IX increased proportionately more than the rest of the porphyrins and coproporphyrin III increased more than coproporphyrin I in the bile of Pb-poisoned mallardsPeer reviewedElsevier201120112004info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/42926reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglésnttp://dx.doi.org/10.1016/j.jchromb.2004.08.019info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/429262026-05-22T06:33:51Z
dc.title.none.fl_str_mv Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography–ultraviolet detection analysis
title Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography–ultraviolet detection analysis
spellingShingle Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography–ultraviolet detection analysis
Mateo, Rafael
Porphyrins
Biliverdin
Lead poisoning
title_short Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography–ultraviolet detection analysis
title_full Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography–ultraviolet detection analysis
title_fullStr Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography–ultraviolet detection analysis
title_full_unstemmed Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography–ultraviolet detection analysis
title_sort Determination of porphyrins and biliverdin in bile and excreta of birds by a single liquid chromatography–ultraviolet detection analysis
dc.creator.none.fl_str_mv Mateo, Rafael
Castells, Gloria
Green, Andy J.
Godoy, Carlo
Cristòfal, Carles
author Mateo, Rafael
author_facet Mateo, Rafael
Castells, Gloria
Green, Andy J.
Godoy, Carlo
Cristòfal, Carles
author_role author
author2 Castells, Gloria
Green, Andy J.
Godoy, Carlo
Cristòfal, Carles
author2_role author
author
author
author
dc.subject.none.fl_str_mv Porphyrins
Biliverdin
Lead poisoning
topic Porphyrins
Biliverdin
Lead poisoning
description Methods developed for porphyrin analysis have low recoveries and/or poor precision for the less polar protoporphyrin IX. We describe a simple method of analysis of porphyrins and biliverdin in bile and excreta of birds based on extraction with HCl 3N: acetonitrile and HPLC/UV analyses. Recoveries were good for protoporphyrin IX and other porphyrins (>79%). Applications of this method showed that porphyrins and biliverdin in birds excreta are mainly of biliary-fecal origin rather than urinary origin. Biliverdin and protoporphyrin IX increased proportionately more than the rest of the porphyrins and coproporphyrin III increased more than coproporphyrin I in the bile of Pb-poisoned mallards
publishDate 2004
dc.date.none.fl_str_mv 2004
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/42926
url http://hdl.handle.net/10261/42926
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv nttp://dx.doi.org/10.1016/j.jchromb.2004.08.019
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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