dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes

H3K4me3 is a histone modification that accumulates at the transcription-start site (TSS) of active genes and is known to be important for transcription activation. The way in which H3K4me3 is regulated at TSS and the actual molecular basis of its contribution to transcription remain largely unanswer...

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Autores: Lloret-Llinares, Marta, Pérez-Lluch, Sílvia, Rossell, David, Morán, Tomás, Ponsà-Cobas, Joan, Auer, Herbert, Corominas, Montserrat (Corominas Guiu), Azorín, F.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/34197
Acceso en línea:https://hdl.handle.net/2445/34197
Access Level:acceso abierto
Palabra clave:Transcripció genètica
Expressió gènica
Genetic transcription
Gene expression
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spelling dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genesLloret-Llinares, MartaPérez-Lluch, SílviaRossell, DavidMorán, TomásPonsà-Cobas, JoanAuer, HerbertCorominas, Montserrat (Corominas Guiu)Azorín, F.Transcripció genèticaExpressió gènicaGenetic transcriptionGene expressionH3K4me3 is a histone modification that accumulates at the transcription-start site (TSS) of active genes and is known to be important for transcription activation. The way in which H3K4me3 is regulated at TSS and the actual molecular basis of its contribution to transcription remain largely unanswered. To address these questions, we have analyzed the contribution of dKDM5/LID, the main H3K4me3 demethylase in Drosophila, to the regulation of the pattern of H3K4me3. ChIP-seq results show that, at developmental genes, dKDM5/LID localizes at TSS and regulates H3K4me3. dKDM5/LID target genes are highly transcribed and enriched in active RNApol II and H3K36me3, suggesting a positive contribution to transcription. Expression-profiling show that, though weakly, dKDM5/LID target genes are significantly downregulated upon dKDM5/LID depletion. Furthermore, dKDM5/LID depletion results in decreased RNApol II occupancy, particularly by the promoter-proximal Pol lloser5 form. Our results also show that ASH2, an evolutionarily conserved factor that locates at TSS and is required for H3K4me3, binds and positively regulates dKDM5/LID target genes. However, dKDM5/LID and ASH2 do not bind simultaneously and recognize different chromatin states, enriched in H3K4me3 and not, respectively. These results indicate that, at developmental genes, dKDM5/LID and ASH2 coordinately regulate H3K4me3 at TSS and that this dynamic regulation contributes to transcription.Oxford University Press2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/34197Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1093/nar/gks773Nucleic Acids Research, 2012, vol. 40, num. 19, p. 9493-9505https://doi.org/10.1093/nar/gks773cc-by-nc (c) Lloret-Llinares, Marta et al., 2012http://creativecommons.org/licenses/by-nc/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/341972026-05-27T06:46:51Z
dc.title.none.fl_str_mv dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes
title dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes
spellingShingle dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes
Lloret-Llinares, Marta
Transcripció genètica
Expressió gènica
Genetic transcription
Gene expression
title_short dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes
title_full dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes
title_fullStr dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes
title_full_unstemmed dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes
title_sort dKDM5/LID regulates H3K4me3 dynamics at the transcription-start site (TSS) of actively transcribed developmental genes
dc.creator.none.fl_str_mv Lloret-Llinares, Marta
Pérez-Lluch, Sílvia
Rossell, David
Morán, Tomás
Ponsà-Cobas, Joan
Auer, Herbert
Corominas, Montserrat (Corominas Guiu)
Azorín, F.
author Lloret-Llinares, Marta
author_facet Lloret-Llinares, Marta
Pérez-Lluch, Sílvia
Rossell, David
Morán, Tomás
Ponsà-Cobas, Joan
Auer, Herbert
Corominas, Montserrat (Corominas Guiu)
Azorín, F.
author_role author
author2 Pérez-Lluch, Sílvia
Rossell, David
Morán, Tomás
Ponsà-Cobas, Joan
Auer, Herbert
Corominas, Montserrat (Corominas Guiu)
Azorín, F.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Transcripció genètica
Expressió gènica
Genetic transcription
Gene expression
topic Transcripció genètica
Expressió gènica
Genetic transcription
Gene expression
description H3K4me3 is a histone modification that accumulates at the transcription-start site (TSS) of active genes and is known to be important for transcription activation. The way in which H3K4me3 is regulated at TSS and the actual molecular basis of its contribution to transcription remain largely unanswered. To address these questions, we have analyzed the contribution of dKDM5/LID, the main H3K4me3 demethylase in Drosophila, to the regulation of the pattern of H3K4me3. ChIP-seq results show that, at developmental genes, dKDM5/LID localizes at TSS and regulates H3K4me3. dKDM5/LID target genes are highly transcribed and enriched in active RNApol II and H3K36me3, suggesting a positive contribution to transcription. Expression-profiling show that, though weakly, dKDM5/LID target genes are significantly downregulated upon dKDM5/LID depletion. Furthermore, dKDM5/LID depletion results in decreased RNApol II occupancy, particularly by the promoter-proximal Pol lloser5 form. Our results also show that ASH2, an evolutionarily conserved factor that locates at TSS and is required for H3K4me3, binds and positively regulates dKDM5/LID target genes. However, dKDM5/LID and ASH2 do not bind simultaneously and recognize different chromatin states, enriched in H3K4me3 and not, respectively. These results indicate that, at developmental genes, dKDM5/LID and ASH2 coordinately regulate H3K4me3 at TSS and that this dynamic regulation contributes to transcription.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/34197
url https://hdl.handle.net/2445/34197
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1093/nar/gks773
Nucleic Acids Research, 2012, vol. 40, num. 19, p. 9493-9505
https://doi.org/10.1093/nar/gks773
dc.rights.none.fl_str_mv cc-by-nc (c) Lloret-Llinares, Marta et al., 2012
http://creativecommons.org/licenses/by-nc/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc (c) Lloret-Llinares, Marta et al., 2012
http://creativecommons.org/licenses/by-nc/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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