Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)

BACKGROUND: The reuse of implant healing abutments is common in dental practice. Effective elimination of bacteria and viruses is accomplished by conventional sterilization. The aim of this work was to explore the eventual survival of microorganisms on sterilized healing abutments and to rule out th...

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Autores: Sánchez Garcés, Ma. Ángeles, Vinuesa Aumedes, Teresa, Ciurana, Joan, Viñas, Miquel
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/171335
Acceso en línea:https://hdl.handle.net/2445/171335
Access Level:acceso abierto
Palabra clave:Implants dentals
Esterilització
Dental implants
Sterilization
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spelling Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)Sánchez Garcés, Ma. ÁngelesVinuesa Aumedes, TeresaCiurana, JoanViñas, MiquelImplants dentalsEsterilitzacióDental implantsSterilizationBACKGROUND: The reuse of implant healing abutments is common in dental practice. Effective elimination of bacteria and viruses is accomplished by conventional sterilization. The aim of this work was to explore the eventual survival of microorganisms on sterilized healing abutments and to rule out the presence of transmissible organic material after standard procedures. MATERIAL AND METHODS: A total of 55 healing abutments previously used in patients will be washed and sterilized in a steam autoclave at 121 C for 15 min. Each healing abutment will be cultured in Brain Heart Infusion broth (BHI) under strict aseptic conditions. Besides, two control groups will be included: one of 3 unused healing abutments, and the other of just medium. After 10 days at 37°C under a 5% CO2 100 µl of the broth will be plated on solid media (Brain Infusion Agar, BHIA) and Columbia Blood agar to test for sterility. The remaining volume will be centrifuged, the sediment fixed, and a Gram stain performed to discard the presence of non-cultivable microorganisms. Moreover, to determine the presence of remaining organic material after the cleaning and sterilizing treatments, the bioburden will be determined by measuring total organic carbon (TOC) in another 10 previously used healing abutments, cleaned and sterilized, that will be submerged in Milli-Q water and sonicated. RESULTS: No bacterial growth was detected on any of the 58 cultured abutments, indicating that the sterilization was completely satisfactory in terms of removal of live bacteria or spores. Nevertheless, significant amounts of organic carbon may still be recovered (up to 125,31 µg/abutment) after they have been sterilized. CONCLUSIONS: Significant amounts of the bioburden remained adhered to the surfaces in spite of the cleaning and sterilization procedures. Taking into account our results and data from other authors, the presence of infectious particles on the reused healing abutments such as prions cannot be ruled out.Medicina Oral SL2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/171335Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.4317/medoral.22967Medicina Oral, Patología Oral y Cirugía Bucal, 2019, vol. 24, num. 5, p. e583-e587https://doi.org/10.4317/medoral.22967(c) Medicina Oral SL, 2019info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1713352026-05-27T06:46:51Z
dc.title.none.fl_str_mv Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)
title Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)
spellingShingle Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)
Sánchez Garcés, Ma. Ángeles
Implants dentals
Esterilització
Dental implants
Sterilization
title_short Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)
title_full Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)
title_fullStr Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)
title_full_unstemmed Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)
title_sort Is the re-use of sterilized implant abutments safe enough? (Implant abutment safety)
dc.creator.none.fl_str_mv Sánchez Garcés, Ma. Ángeles
Vinuesa Aumedes, Teresa
Ciurana, Joan
Viñas, Miquel
author Sánchez Garcés, Ma. Ángeles
author_facet Sánchez Garcés, Ma. Ángeles
Vinuesa Aumedes, Teresa
Ciurana, Joan
Viñas, Miquel
author_role author
author2 Vinuesa Aumedes, Teresa
Ciurana, Joan
Viñas, Miquel
author2_role author
author
author
dc.subject.none.fl_str_mv Implants dentals
Esterilització
Dental implants
Sterilization
topic Implants dentals
Esterilització
Dental implants
Sterilization
description BACKGROUND: The reuse of implant healing abutments is common in dental practice. Effective elimination of bacteria and viruses is accomplished by conventional sterilization. The aim of this work was to explore the eventual survival of microorganisms on sterilized healing abutments and to rule out the presence of transmissible organic material after standard procedures. MATERIAL AND METHODS: A total of 55 healing abutments previously used in patients will be washed and sterilized in a steam autoclave at 121 C for 15 min. Each healing abutment will be cultured in Brain Heart Infusion broth (BHI) under strict aseptic conditions. Besides, two control groups will be included: one of 3 unused healing abutments, and the other of just medium. After 10 days at 37°C under a 5% CO2 100 µl of the broth will be plated on solid media (Brain Infusion Agar, BHIA) and Columbia Blood agar to test for sterility. The remaining volume will be centrifuged, the sediment fixed, and a Gram stain performed to discard the presence of non-cultivable microorganisms. Moreover, to determine the presence of remaining organic material after the cleaning and sterilizing treatments, the bioburden will be determined by measuring total organic carbon (TOC) in another 10 previously used healing abutments, cleaned and sterilized, that will be submerged in Milli-Q water and sonicated. RESULTS: No bacterial growth was detected on any of the 58 cultured abutments, indicating that the sterilization was completely satisfactory in terms of removal of live bacteria or spores. Nevertheless, significant amounts of organic carbon may still be recovered (up to 125,31 µg/abutment) after they have been sterilized. CONCLUSIONS: Significant amounts of the bioburden remained adhered to the surfaces in spite of the cleaning and sterilization procedures. Taking into account our results and data from other authors, the presence of infectious particles on the reused healing abutments such as prions cannot be ruled out.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/171335
url https://hdl.handle.net/2445/171335
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.4317/medoral.22967
Medicina Oral, Patología Oral y Cirugía Bucal, 2019, vol. 24, num. 5, p. e583-e587
https://doi.org/10.4317/medoral.22967
dc.rights.none.fl_str_mv (c) Medicina Oral SL, 2019
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Medicina Oral SL, 2019
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Medicina Oral SL
publisher.none.fl_str_mv Medicina Oral SL
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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