Dietary protein: lipid ratio modulates somatic growth and expression of genes involved in somatic growth, lipid metabolism and food intake in Pejerrey fry (Odontesthes bonariensis)

Pejerrey is a freshwater fish from South America with high potential for aquaculture. This study was designed to determine the effects of different dietary protein:lipid ratio on growth rate and the expression of growth, lipid metabolism and feeding-related genes of this species during early develop...

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Detalles Bibliográficos
Autores: Bertucci, Juan Ignacio, Blanco, Ayelén Melisa, Navarro, Juan Carlos, Unniappan, Suraj, Canosa, Luis Fabián
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/284661
Acceso en línea:http://hdl.handle.net/10261/284661
Access Level:acceso abierto
Palabra clave:Δ6-desaturase
Growth hormone
Insulin-like growth factor
Lipid
Nucb2/nesfatin-1
Protein
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spelling Dietary protein: lipid ratio modulates somatic growth and expression of genes involved in somatic growth, lipid metabolism and food intake in Pejerrey fry (Odontesthes bonariensis)Bertucci, Juan IgnacioBlanco, Ayelén MelisaNavarro, Juan CarlosUnniappan, SurajCanosa, Luis FabiánΔ6-desaturaseGrowth hormoneInsulin-like growth factorLipidNucb2/nesfatin-1ProteinPejerrey is a freshwater fish from South America with high potential for aquaculture. This study was designed to determine the effects of different dietary protein:lipid ratio on growth rate and the expression of growth, lipid metabolism and feeding-related genes of this species during early developmental stages. Pejerrey fry were fed for 60 days with four experimental diets containing low (400 g Kg) or high (500 g Kg) protein (LP or HP, respectively) and low (120 g Kg) or high (200 g Kg) lipid (LL or HL, respectively), in the combinations: LP-LL; LP-HL; HP-LL and HP-HL. Measurements of growth, lipid and fatty acid content of fry, expression of genes from the endocrine axis (gh, ghrs, igfs), fatty acid metabolism (∆6-desaturase), and food intake behavior (nucb2/nesfatin-1) were collected. Fry fed with diets LP-LL and HP-LL showed the highest growth rate and growth hormone (gh) mRNA expression levels. The gene expression of ∆6-desaturase was high in head of fry fed with diet LP-HL. The mRNA expression of nucb2/nesfatin-1 and gh followed the same patterns in head, and the inverse pattern in body. In conclusion, diets with LL ensure a higher growth of pejerrey fry compared to those that contain HL, without altering the final lipid amount nor the fatty acid profile on fry. In LL groups, the expression of genes from the GH-IGF axis is associated with the observed promotion of somatic growth. The expression of nucb2/nesfatin-1 indicates an effect of this peptide not related to food intake regulation, e.g., a negative regulatory role on GH expression, that would warrant future research.This work was partially funded by Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT, Argentina; PICT 2010–1493) and Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET; PIP 2014/0595) to LFC, and a Discovery grant, and Discovery Accelerator Supplement award from the Natural Sciences and Engineering Research Council (NSERC) of Canada to SU. SU received a new investigator salary award from the Canadian Institutes of Health Research (CIHR). JIB was recipient of a doctoral fellowship from ANPCyT (Argentina), and from the Emerging Leaders of the Americas Program (ELAP) scholarship from the Government of Canada and doctoral fellow from CONICET (Argentina).ElsevierAgencia Nacional de Promoción Científica y Tecnológica (Argentina)Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina)Natural Sciences and Engineering Research Council of CanadaCanadian Institutes of Health ResearchGovernment of CanadaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/284661reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.cbpa.2022.111231Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2846612026-05-22T06:33:51Z
dc.title.none.fl_str_mv Dietary protein: lipid ratio modulates somatic growth and expression of genes involved in somatic growth, lipid metabolism and food intake in Pejerrey fry (Odontesthes bonariensis)
title Dietary protein: lipid ratio modulates somatic growth and expression of genes involved in somatic growth, lipid metabolism and food intake in Pejerrey fry (Odontesthes bonariensis)
spellingShingle Dietary protein: lipid ratio modulates somatic growth and expression of genes involved in somatic growth, lipid metabolism and food intake in Pejerrey fry (Odontesthes bonariensis)
Bertucci, Juan Ignacio
Δ6-desaturase
Growth hormone
Insulin-like growth factor
Lipid
Nucb2/nesfatin-1
Protein
title_short Dietary protein: lipid ratio modulates somatic growth and expression of genes involved in somatic growth, lipid metabolism and food intake in Pejerrey fry (Odontesthes bonariensis)
title_full Dietary protein: lipid ratio modulates somatic growth and expression of genes involved in somatic growth, lipid metabolism and food intake in Pejerrey fry (Odontesthes bonariensis)
title_fullStr Dietary protein: lipid ratio modulates somatic growth and expression of genes involved in somatic growth, lipid metabolism and food intake in Pejerrey fry (Odontesthes bonariensis)
title_full_unstemmed Dietary protein: lipid ratio modulates somatic growth and expression of genes involved in somatic growth, lipid metabolism and food intake in Pejerrey fry (Odontesthes bonariensis)
title_sort Dietary protein: lipid ratio modulates somatic growth and expression of genes involved in somatic growth, lipid metabolism and food intake in Pejerrey fry (Odontesthes bonariensis)
dc.creator.none.fl_str_mv Bertucci, Juan Ignacio
Blanco, Ayelén Melisa
Navarro, Juan Carlos
Unniappan, Suraj
Canosa, Luis Fabián
author Bertucci, Juan Ignacio
author_facet Bertucci, Juan Ignacio
Blanco, Ayelén Melisa
Navarro, Juan Carlos
Unniappan, Suraj
Canosa, Luis Fabián
author_role author
author2 Blanco, Ayelén Melisa
Navarro, Juan Carlos
Unniappan, Suraj
Canosa, Luis Fabián
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Agencia Nacional de Promoción Científica y Tecnológica (Argentina)
Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina)
Natural Sciences and Engineering Research Council of Canada
Canadian Institutes of Health Research
Government of Canada
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Δ6-desaturase
Growth hormone
Insulin-like growth factor
Lipid
Nucb2/nesfatin-1
Protein
topic Δ6-desaturase
Growth hormone
Insulin-like growth factor
Lipid
Nucb2/nesfatin-1
Protein
description Pejerrey is a freshwater fish from South America with high potential for aquaculture. This study was designed to determine the effects of different dietary protein:lipid ratio on growth rate and the expression of growth, lipid metabolism and feeding-related genes of this species during early developmental stages. Pejerrey fry were fed for 60 days with four experimental diets containing low (400 g Kg) or high (500 g Kg) protein (LP or HP, respectively) and low (120 g Kg) or high (200 g Kg) lipid (LL or HL, respectively), in the combinations: LP-LL; LP-HL; HP-LL and HP-HL. Measurements of growth, lipid and fatty acid content of fry, expression of genes from the endocrine axis (gh, ghrs, igfs), fatty acid metabolism (∆6-desaturase), and food intake behavior (nucb2/nesfatin-1) were collected. Fry fed with diets LP-LL and HP-LL showed the highest growth rate and growth hormone (gh) mRNA expression levels. The gene expression of ∆6-desaturase was high in head of fry fed with diet LP-HL. The mRNA expression of nucb2/nesfatin-1 and gh followed the same patterns in head, and the inverse pattern in body. In conclusion, diets with LL ensure a higher growth of pejerrey fry compared to those that contain HL, without altering the final lipid amount nor the fatty acid profile on fry. In LL groups, the expression of genes from the GH-IGF axis is associated with the observed promotion of somatic growth. The expression of nucb2/nesfatin-1 indicates an effect of this peptide not related to food intake regulation, e.g., a negative regulatory role on GH expression, that would warrant future research.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/284661
url http://hdl.handle.net/10261/284661
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.cbpa.2022.111231
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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