Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markers

Genetic relationships among 40 loquat (Eriobotrya japonica (Thunb) Lindl) accessions that originated from different countries and that are part of the germplasm collection of the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) were evaluated using microsatellites. Thirty pr...

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Authors: Soriano Soriano, José Miguel, Romero, Carlos, Vilanova, Santiago, Llácer Ill, Gerardo, Badenes, Maria Luisa
Format: article
Status:Versión aceptada para publicación
Publication Date:2005
Country:España
Institution:Universitat de Lleida (UdL)
Repository:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/465968
Online Access:https://doi.org/10.1139/G04-101
https://hdl.handle.net/10459.1/465968
Access Level:Open access
Keyword:Eriobotrya japonica
SSR markers
Microsatellites
Genetic diversity
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spelling Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markersSoriano Soriano, José MiguelRomero, CarlosVilanova, SantiagoLlácer Ill, GerardoBadenes, Maria LuisaEriobotrya japonicaSSR markersMicrosatellitesGenetic diversityGenetic relationships among 40 loquat (Eriobotrya japonica (Thunb) Lindl) accessions that originated from different countries and that are part of the germplasm collection of the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) were evaluated using microsatellites. Thirty primer pairs flanking microsatellites previously identified in Malus × domestica (Borkh.) were assayed. Thirteen of them amplified polymorphic products and unambiguously distinguished 34 genotypes from the 40 accessions analyzed. Six accessions showing identical marker patterns were Spanish local varieties thought to have been derived from 'Algerie' by a mutational process very common in loquat species. A total of 39 alleles were detected in the population studied, with a mean value of 2.4 alleles per locus. The expected and observed heterozygosities were 0.46 and 51% on average, respectively, leading to a negative value of the Wright's fixation index (-0.20). The values of these parameters indicate a smaller degree of genetic diversity in the set of loquat accessions analyzed than in other members of the Rosaceae family. Unweighted pair-group method (UPGMA) cluster analysis, based on Nei's genetic distance, generally grouped genotypes according to their geographic origins and pedigrees. The high number of alleles and the high expected heterozygosity detected with SSR markers developed in Malus × domestica (Borkh.) make them a suitable tool for loquat cultivar identification, confirming microsatellite marker transportability among genera in the Rosaceae family.The authors thank Dr. L. Georgi for helpful comments that improved the manuscript. This research has been funded by the grant INIA RF01-018 from the Ministerio de Ciencia y Tecnología.Canadian Science Publishing2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://doi.org/10.1139/G04-101https://hdl.handle.net/10459.1/465968reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)InglésReproducció del document publicat a: https://doi.org/10.1139/G04-101Genome, 2005, vol. 48 núm. 1, p. 108-114cc-by, (c) Soriano et al., 2005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/4.0/deed.caoai:repositori.udl.cat:10459.1/4659682026-06-24T12:42:17Z
dc.title.none.fl_str_mv Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markers
title Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markers
spellingShingle Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markers
Soriano Soriano, José Miguel
Eriobotrya japonica
SSR markers
Microsatellites
Genetic diversity
title_short Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markers
title_full Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markers
title_fullStr Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markers
title_full_unstemmed Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markers
title_sort Genetic diversity of loquat germplasm (Eriobotrya japonica (Thunb) Lindl) assessed by SSR markers
dc.creator.none.fl_str_mv Soriano Soriano, José Miguel
Romero, Carlos
Vilanova, Santiago
Llácer Ill, Gerardo
Badenes, Maria Luisa
author Soriano Soriano, José Miguel
author_facet Soriano Soriano, José Miguel
Romero, Carlos
Vilanova, Santiago
Llácer Ill, Gerardo
Badenes, Maria Luisa
author_role author
author2 Romero, Carlos
Vilanova, Santiago
Llácer Ill, Gerardo
Badenes, Maria Luisa
author2_role author
author
author
author
dc.subject.none.fl_str_mv Eriobotrya japonica
SSR markers
Microsatellites
Genetic diversity
topic Eriobotrya japonica
SSR markers
Microsatellites
Genetic diversity
description Genetic relationships among 40 loquat (Eriobotrya japonica (Thunb) Lindl) accessions that originated from different countries and that are part of the germplasm collection of the Instituto Valenciano de Investigaciones Agrarias (IVIA) (Valencia, Spain) were evaluated using microsatellites. Thirty primer pairs flanking microsatellites previously identified in Malus × domestica (Borkh.) were assayed. Thirteen of them amplified polymorphic products and unambiguously distinguished 34 genotypes from the 40 accessions analyzed. Six accessions showing identical marker patterns were Spanish local varieties thought to have been derived from 'Algerie' by a mutational process very common in loquat species. A total of 39 alleles were detected in the population studied, with a mean value of 2.4 alleles per locus. The expected and observed heterozygosities were 0.46 and 51% on average, respectively, leading to a negative value of the Wright's fixation index (-0.20). The values of these parameters indicate a smaller degree of genetic diversity in the set of loquat accessions analyzed than in other members of the Rosaceae family. Unweighted pair-group method (UPGMA) cluster analysis, based on Nei's genetic distance, generally grouped genotypes according to their geographic origins and pedigrees. The high number of alleles and the high expected heterozygosity detected with SSR markers developed in Malus × domestica (Borkh.) make them a suitable tool for loquat cultivar identification, confirming microsatellite marker transportability among genera in the Rosaceae family.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1139/G04-101
https://hdl.handle.net/10459.1/465968
url https://doi.org/10.1139/G04-101
https://hdl.handle.net/10459.1/465968
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1139/G04-101
Genome, 2005, vol. 48 núm. 1, p. 108-114
dc.rights.none.fl_str_mv cc-by, (c) Soriano et al., 2005
info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/4.0/deed.ca
rights_invalid_str_mv cc-by, (c) Soriano et al., 2005
https://creativecommons.org/licenses/by/4.0/deed.ca
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Canadian Science Publishing
publisher.none.fl_str_mv Canadian Science Publishing
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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