Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits

[EN] Swine viral diseases challenge the sector's sustainability by affecting productivity and the health and welfare of the animals. The lack of antiviral drugs and/or effective vaccines renders early and reliable diagnosis the basis of viral disease management, underlining the importance o...

Descripción completa

Detalles Bibliográficos
Autores: Manessis, Georgios, Frant, Maiej, Wozniakowski, Grzegrorz, Nannucci, Lapo, Bennedetti, Martina, Denes, Lilla, Balka, Gyula, Gelasakis, Anathasios I., Squires, Clare, Recuero, Sara, Sanchez, Carlos, Giusti, Alessandro, Bossis, Ioannis, Griol Barres, Amadeu
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/194258
Acceso en línea:https://riunet.upv.es/handle/10251/194258
Access Level:acceso abierto
Palabra clave:Point of care
Diagnostics
Photonic integrated circuits
Microfluidics
Porcine reproductive and respiratory syndrome virus
Swine influenza A virus
Oral fluids
Validation
Sensitivity
Specificity
Diagnostic odds ratio
02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
id ES_6a33a2234fbf6f3eabd80bcdca0a0d1b
oai_identifier_str oai:riunet.upv.es:10251/194258
network_acronym_str ES
network_name_str España
repository_id_str
spelling Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated CircuitsManessis, GeorgiosFrant, MaiejWozniakowski, GrzegrorzNannucci, LapoBennedetti, MartinaDenes, LillaBalka, GyulaGelasakis, Anathasios I.Squires, ClareRecuero, SaraSanchez, CarlosGiusti, AlessandroBossis, IoannisGriol Barres, AmadeuPoint of careDiagnosticsPhotonic integrated circuitsMicrofluidicsPorcine reproductive and respiratory syndrome virusSwine influenza A virusOral fluidsValidationSensitivitySpecificityDiagnostic odds ratio02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades[EN] Swine viral diseases challenge the sector's sustainability by affecting productivity and the health and welfare of the animals. The lack of antiviral drugs and/or effective vaccines renders early and reliable diagnosis the basis of viral disease management, underlining the importance of point-of-care (POC) diagnostics. A novel POC diagnostic device utilizing photonic integrated circuits (PICs), microfluidics, and information and communication technologies for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza A (SIV) was validated using spiked and clinical oral fluid samples. Metrics including sensitivity, specificity, accuracy, precision, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) were calculated to assess the performance of the device. For PRRSV, the device achieved a sensitivity of 83.5%, specificity of 77.8%, and DOR values of 17.66, whereas the values for SIV were 81.8%, 82.2%, and 20.81, respectively. The POC device and PICs can be used for the detection of PRRSV and SIV in the field, paving the way for the introduction of novel technologies in the field of animal POC diagnostics to further optimize livestock biosecurity.This research was funded by the EU's H2020 SWINOSTICS project under the grant agreement ID 771649.MDPI AGInstituto Universitario de Tecnología NanofotónicaCOMISION DE LAS COMUNIDADES EUROPEARepositorio Institucional de la Universitat Politècnica de València Riunet20222022-05-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/194258reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengEuropean Commission https://doi.org/10.13039/501100000780 H2020 771649open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento (by)http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1942582026-06-13T07:49:27Z
dc.title.none.fl_str_mv Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits
title Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits
spellingShingle Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits
Manessis, Georgios
Point of care
Diagnostics
Photonic integrated circuits
Microfluidics
Porcine reproductive and respiratory syndrome virus
Swine influenza A virus
Oral fluids
Validation
Sensitivity
Specificity
Diagnostic odds ratio
02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
title_short Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits
title_full Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits
title_fullStr Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits
title_full_unstemmed Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits
title_sort Point-of-Care and Label-Free Detection of Porcine Reproductive and Respiratory Syndrome and Swine Influenza Viruses Using a Microfluidic Device with Photonic Integrated Circuits
dc.creator.none.fl_str_mv Manessis, Georgios
Frant, Maiej
Wozniakowski, Grzegrorz
Nannucci, Lapo
Bennedetti, Martina
Denes, Lilla
Balka, Gyula
Gelasakis, Anathasios I.
Squires, Clare
Recuero, Sara
Sanchez, Carlos
Giusti, Alessandro
Bossis, Ioannis
Griol Barres, Amadeu
author Manessis, Georgios
author_facet Manessis, Georgios
Frant, Maiej
Wozniakowski, Grzegrorz
Nannucci, Lapo
Bennedetti, Martina
Denes, Lilla
Balka, Gyula
Gelasakis, Anathasios I.
Squires, Clare
Recuero, Sara
Sanchez, Carlos
Giusti, Alessandro
Bossis, Ioannis
Griol Barres, Amadeu
author_role author
author2 Frant, Maiej
Wozniakowski, Grzegrorz
Nannucci, Lapo
Bennedetti, Martina
Denes, Lilla
Balka, Gyula
Gelasakis, Anathasios I.
Squires, Clare
Recuero, Sara
Sanchez, Carlos
Giusti, Alessandro
Bossis, Ioannis
Griol Barres, Amadeu
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario de Tecnología Nanofotónica
COMISION DE LAS COMUNIDADES EUROPEA
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Point of care
Diagnostics
Photonic integrated circuits
Microfluidics
Porcine reproductive and respiratory syndrome virus
Swine influenza A virus
Oral fluids
Validation
Sensitivity
Specificity
Diagnostic odds ratio
02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
topic Point of care
Diagnostics
Photonic integrated circuits
Microfluidics
Porcine reproductive and respiratory syndrome virus
Swine influenza A virus
Oral fluids
Validation
Sensitivity
Specificity
Diagnostic odds ratio
02.- Poner fin al hambre, conseguir la seguridad alimentaria y una mejor nutrición, y promover la agricultura sostenible
03.- Garantizar una vida saludable y promover el bienestar para todos y todas en todas las edades
description [EN] Swine viral diseases challenge the sector's sustainability by affecting productivity and the health and welfare of the animals. The lack of antiviral drugs and/or effective vaccines renders early and reliable diagnosis the basis of viral disease management, underlining the importance of point-of-care (POC) diagnostics. A novel POC diagnostic device utilizing photonic integrated circuits (PICs), microfluidics, and information and communication technologies for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) and swine influenza A (SIV) was validated using spiked and clinical oral fluid samples. Metrics including sensitivity, specificity, accuracy, precision, positive likelihood ratio (PLR), negative likelihood ratio (NLR), and diagnostic odds ratio (DOR) were calculated to assess the performance of the device. For PRRSV, the device achieved a sensitivity of 83.5%, specificity of 77.8%, and DOR values of 17.66, whereas the values for SIV were 81.8%, 82.2%, and 20.81, respectively. The POC device and PICs can be used for the detection of PRRSV and SIV in the field, paving the way for the introduction of novel technologies in the field of animal POC diagnostics to further optimize livestock biosecurity.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-05-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/194258
url https://riunet.upv.es/handle/10251/194258
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv European Commission https://doi.org/10.13039/501100000780 H2020 771649
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento (by)
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410083336617984
score 15.300719

Similares en LA Referencia