Purine-related metabolites and their converting enzymes are altered in frontal; parietal; and temporal cortex at early stages of Alzheimer's disease pathology

Adenosine, hypoxanthine, xanthine, guanosine, and inosine levels were assessed by HPLC, and the activity of related enzymes 5'-nucleotidase (5'-NT), adenosine deaminase (ADA), and purine nucleoside phosphorylase (PNP) measured in frontal (FC), parietal (PC) and temporal (TC) cortices at di...

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Autores: Alonso-Andrés, Patricia, Albasanz, José Luis, Ferrer, Isidro (Ferrer Abizanda), Martín, Mairena
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/140360
Acceso en línea:https://hdl.handle.net/2445/140360
Access Level:acceso abierto
Palabra clave:Malaltia d'Alzheimer
Enzimologia
Lòbul frontal
Lòbul temporal
Metabolisme
Purines
Alzheimer's disease
Enzymology
Frontal lobe
Temporal lobe
Metabolism
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spelling Purine-related metabolites and their converting enzymes are altered in frontal; parietal; and temporal cortex at early stages of Alzheimer's disease pathologyAlonso-Andrés, PatriciaAlbasanz, José LuisFerrer, Isidro (Ferrer Abizanda)Martín, MairenaMalaltia d'AlzheimerEnzimologiaLòbul frontalLòbul temporalMetabolismePurinesAlzheimer's diseaseEnzymologyFrontal lobeTemporal lobeMetabolismPurinesAdenosine, hypoxanthine, xanthine, guanosine, and inosine levels were assessed by HPLC, and the activity of related enzymes 5'-nucleotidase (5'-NT), adenosine deaminase (ADA), and purine nucleoside phosphorylase (PNP) measured in frontal (FC), parietal (PC) and temporal (TC) cortices at different stages of disease progression in Alzheimer's disease (AD) and in age-matched controls. Significantly decreased levels of adenosine, guanosine, hypoxanthine, and xanthine, and apparently less inosine, are found in FC from the early stages of AD; PC and TC show an opposing pattern, as adenosine, guanosine, and inosine are significantly increased at least at determinate stages of AD whereas hypoxanthine and xanthine levels remain unaltered. 5'-NT is reduced in membranes and cytosol in FC mainly at early stages but not in PC, and only at advanced stages in cytosol in TC. ADA activity is decreased in AD when considered as a whole but increased at early stages in TC. Finally, PNP activity is increased only in TC at early stages. Purine metabolism alterations occur at early stages of AD independently of neurofibrillary tangles and β-amyloid plaques. Alterations are stage- and regiondependent, the latter showing opposite patterns in FC compared with PC and TC. Adenosine is the most affected of the assessed purines. Key words: Adenosine, Alzheimer's disease, cerebral cortex, purine metabolism.Wiley2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/140360Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1111/bpa.12592Brain Pathology, 2018, vol. 28, num. 6, p. 933-946https://doi.org/10.1111/bpa.12592(c) International Society of Neuropathology, 2018info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1403602026-05-27T06:46:51Z
dc.title.none.fl_str_mv Purine-related metabolites and their converting enzymes are altered in frontal; parietal; and temporal cortex at early stages of Alzheimer's disease pathology
title Purine-related metabolites and their converting enzymes are altered in frontal; parietal; and temporal cortex at early stages of Alzheimer's disease pathology
spellingShingle Purine-related metabolites and their converting enzymes are altered in frontal; parietal; and temporal cortex at early stages of Alzheimer's disease pathology
Alonso-Andrés, Patricia
Malaltia d'Alzheimer
Enzimologia
Lòbul frontal
Lòbul temporal
Metabolisme
Purines
Alzheimer's disease
Enzymology
Frontal lobe
Temporal lobe
Metabolism
Purines
title_short Purine-related metabolites and their converting enzymes are altered in frontal; parietal; and temporal cortex at early stages of Alzheimer's disease pathology
title_full Purine-related metabolites and their converting enzymes are altered in frontal; parietal; and temporal cortex at early stages of Alzheimer's disease pathology
title_fullStr Purine-related metabolites and their converting enzymes are altered in frontal; parietal; and temporal cortex at early stages of Alzheimer's disease pathology
title_full_unstemmed Purine-related metabolites and their converting enzymes are altered in frontal; parietal; and temporal cortex at early stages of Alzheimer's disease pathology
title_sort Purine-related metabolites and their converting enzymes are altered in frontal; parietal; and temporal cortex at early stages of Alzheimer's disease pathology
dc.creator.none.fl_str_mv Alonso-Andrés, Patricia
Albasanz, José Luis
Ferrer, Isidro (Ferrer Abizanda)
Martín, Mairena
author Alonso-Andrés, Patricia
author_facet Alonso-Andrés, Patricia
Albasanz, José Luis
Ferrer, Isidro (Ferrer Abizanda)
Martín, Mairena
author_role author
author2 Albasanz, José Luis
Ferrer, Isidro (Ferrer Abizanda)
Martín, Mairena
author2_role author
author
author
dc.subject.none.fl_str_mv Malaltia d'Alzheimer
Enzimologia
Lòbul frontal
Lòbul temporal
Metabolisme
Purines
Alzheimer's disease
Enzymology
Frontal lobe
Temporal lobe
Metabolism
Purines
topic Malaltia d'Alzheimer
Enzimologia
Lòbul frontal
Lòbul temporal
Metabolisme
Purines
Alzheimer's disease
Enzymology
Frontal lobe
Temporal lobe
Metabolism
Purines
description Adenosine, hypoxanthine, xanthine, guanosine, and inosine levels were assessed by HPLC, and the activity of related enzymes 5'-nucleotidase (5'-NT), adenosine deaminase (ADA), and purine nucleoside phosphorylase (PNP) measured in frontal (FC), parietal (PC) and temporal (TC) cortices at different stages of disease progression in Alzheimer's disease (AD) and in age-matched controls. Significantly decreased levels of adenosine, guanosine, hypoxanthine, and xanthine, and apparently less inosine, are found in FC from the early stages of AD; PC and TC show an opposing pattern, as adenosine, guanosine, and inosine are significantly increased at least at determinate stages of AD whereas hypoxanthine and xanthine levels remain unaltered. 5'-NT is reduced in membranes and cytosol in FC mainly at early stages but not in PC, and only at advanced stages in cytosol in TC. ADA activity is decreased in AD when considered as a whole but increased at early stages in TC. Finally, PNP activity is increased only in TC at early stages. Purine metabolism alterations occur at early stages of AD independently of neurofibrillary tangles and β-amyloid plaques. Alterations are stage- and regiondependent, the latter showing opposite patterns in FC compared with PC and TC. Adenosine is the most affected of the assessed purines. Key words: Adenosine, Alzheimer's disease, cerebral cortex, purine metabolism.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/140360
url https://hdl.handle.net/2445/140360
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1111/bpa.12592
Brain Pathology, 2018, vol. 28, num. 6, p. 933-946
https://doi.org/10.1111/bpa.12592
dc.rights.none.fl_str_mv (c) International Society of Neuropathology, 2018
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) International Society of Neuropathology, 2018
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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