Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors

The phylum Chloroflexi is highly abundant in a wide variety of wastewater treatment bioreactors. It has been suggested that they play relevant roles in these ecosystems, particularly in degrading carbon compounds and on structuring flocs or granules. Nevertheless, their function is not yet well unde...

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Autores: Bovio-Winkler, Patricia|||0000-0002-4004-0416, Guerrero, Leandro D., Erijman, Leonardo|||0000-0001-7525-2112, Oyarzúa Alarcón, Pía Karina|||0000-0002-0892-4174, Suárez Ojeda, María Eugenia|||0000-0003-2520-2701, Cabezas, Angela|||0000-0001-5460-019X, Etchebehere, Claudia|||0000-0003-2495-205X
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:292084
Acceso en línea:https://ddd.uab.cat/record/292084
https://dx.doi.org/urn:doi:10.1186/s12866-023-02765-5
Access Level:acceso abierto
Palabra clave:Activated sludge
Anammox
Chloroflexi
Metagenome assembled genomes
Methanogenic reactors
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spelling Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactorsBovio-Winkler, Patricia|||0000-0002-4004-0416Guerrero, Leandro D.Erijman, Leonardo|||0000-0001-7525-2112Oyarzúa Alarcón, Pía Karina|||0000-0002-0892-4174Suárez Ojeda, María Eugenia|||0000-0003-2520-2701Cabezas, Angela|||0000-0001-5460-019XEtchebehere, Claudia|||0000-0003-2495-205XActivated sludgeAnammoxChloroflexiMetagenome assembled genomesMethanogenic reactorsThe phylum Chloroflexi is highly abundant in a wide variety of wastewater treatment bioreactors. It has been suggested that they play relevant roles in these ecosystems, particularly in degrading carbon compounds and on structuring flocs or granules. Nevertheless, their function is not yet well understood as most species have not been isolated in axenic cultures. Here we used a metagenomic approach to investigate Chloroflexi diversity and their metabolic potential in three environmentally different bioreactors: a methanogenic full-scale reactor, a full-scale activated sludge reactor and a lab scale anammox reactor. Differential coverage binning approach was used to assemble the genomes of 17 new Chloroflexi species, two of which are proposed as new Candidatus genus. In addition, we recovered the first representative genome belonging to the genus 'Ca. Villigracilis'. Even though samples analyzed were collected from bioreactors operating under different environmental conditions, the assembled genomes share several metabolic features: anaerobic metabolism, fermentative pathways and several genes coding for hydrolytic enzymes. Interestingly, genome analysis from the anammox reactor indicated a putative role of Chloroflexi in nitrogen conversion. Genes related to adhesiveness and exopolysaccharides production were also detected. Complementing sequencing analysis, filamentous morphology was detected by Fluorescent in situ hybridization. Our results suggest that Chloroflexi participate in organic matter degradation, nitrogen removal and biofilm aggregation, playing different roles according to the environmental conditions. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/292084https://dx.doi.org/urn:doi:10.1186/s12866-023-02765-5reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2920842026-06-06T12:50:31Z
dc.title.none.fl_str_mv Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors
title Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors
spellingShingle Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors
Bovio-Winkler, Patricia|||0000-0002-4004-0416
Activated sludge
Anammox
Chloroflexi
Metagenome assembled genomes
Methanogenic reactors
title_short Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors
title_full Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors
title_fullStr Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors
title_full_unstemmed Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors
title_sort Genome-centric metagenomic insights into the role of Chloroflexi in anammox, activated sludge and methanogenic reactors
dc.creator.none.fl_str_mv Bovio-Winkler, Patricia|||0000-0002-4004-0416
Guerrero, Leandro D.
Erijman, Leonardo|||0000-0001-7525-2112
Oyarzúa Alarcón, Pía Karina|||0000-0002-0892-4174
Suárez Ojeda, María Eugenia|||0000-0003-2520-2701
Cabezas, Angela|||0000-0001-5460-019X
Etchebehere, Claudia|||0000-0003-2495-205X
author Bovio-Winkler, Patricia|||0000-0002-4004-0416
author_facet Bovio-Winkler, Patricia|||0000-0002-4004-0416
Guerrero, Leandro D.
Erijman, Leonardo|||0000-0001-7525-2112
Oyarzúa Alarcón, Pía Karina|||0000-0002-0892-4174
Suárez Ojeda, María Eugenia|||0000-0003-2520-2701
Cabezas, Angela|||0000-0001-5460-019X
Etchebehere, Claudia|||0000-0003-2495-205X
author_role author
author2 Guerrero, Leandro D.
Erijman, Leonardo|||0000-0001-7525-2112
Oyarzúa Alarcón, Pía Karina|||0000-0002-0892-4174
Suárez Ojeda, María Eugenia|||0000-0003-2520-2701
Cabezas, Angela|||0000-0001-5460-019X
Etchebehere, Claudia|||0000-0003-2495-205X
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Activated sludge
Anammox
Chloroflexi
Metagenome assembled genomes
Methanogenic reactors
topic Activated sludge
Anammox
Chloroflexi
Metagenome assembled genomes
Methanogenic reactors
description The phylum Chloroflexi is highly abundant in a wide variety of wastewater treatment bioreactors. It has been suggested that they play relevant roles in these ecosystems, particularly in degrading carbon compounds and on structuring flocs or granules. Nevertheless, their function is not yet well understood as most species have not been isolated in axenic cultures. Here we used a metagenomic approach to investigate Chloroflexi diversity and their metabolic potential in three environmentally different bioreactors: a methanogenic full-scale reactor, a full-scale activated sludge reactor and a lab scale anammox reactor. Differential coverage binning approach was used to assemble the genomes of 17 new Chloroflexi species, two of which are proposed as new Candidatus genus. In addition, we recovered the first representative genome belonging to the genus 'Ca. Villigracilis'. Even though samples analyzed were collected from bioreactors operating under different environmental conditions, the assembled genomes share several metabolic features: anaerobic metabolism, fermentative pathways and several genes coding for hydrolytic enzymes. Interestingly, genome analysis from the anammox reactor indicated a putative role of Chloroflexi in nitrogen conversion. Genes related to adhesiveness and exopolysaccharides production were also detected. Complementing sequencing analysis, filamentous morphology was detected by Fluorescent in situ hybridization. Our results suggest that Chloroflexi participate in organic matter degradation, nitrogen removal and biofilm aggregation, playing different roles according to the environmental conditions.
publishDate 2023
dc.date.none.fl_str_mv 2
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/292084
https://dx.doi.org/urn:doi:10.1186/s12866-023-02765-5
url https://ddd.uab.cat/record/292084
https://dx.doi.org/urn:doi:10.1186/s12866-023-02765-5
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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