A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts
© 2020. Published by The Company of Biologists Ltd.
| Autores: | , , , , , , , , , , , , , , , , |
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| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Consejo Superior de Investigaciones Científicas (CSIC) |
| Repositorio: | DIGITAL.CSIC. Repositorio Institucional del CSIC |
| OAI Identifier: | oai:digital.csic.es:10261/226604 |
| Acceso en línea: | http://hdl.handle.net/10261/226604 |
| Access Level: | acceso abierto |
| Palabra clave: | Mass spectrometry SWATH-MS Proteomics Mouse Xenografts DCIS Breast cancer |
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A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenograftsKrasny, LukasBland, PhilipBurns, JessicaCarvalho Lima, NadiaHarrison, Peter T.Pacini, LauraElms, Mark L.Ning, JianGarcia Martinez, VictorYu, Yi-RuActon, Sophie E.Ho, Ping-ChihCalvo, FernandoSwain, AmandaHoward, Beatrice A.Natrajan, Rachael C.Huang, Paul H.Mass spectrometrySWATH-MSProteomicsMouseXenograftsDCISBreast cancer© 2020. Published by The Company of Biologists Ltd.SWATH-mass spectrometry (MS) enables accurate and reproducible proteomic profiling in multiple model organisms including the mouse. Here, we present a comprehensive mouse reference spectral library (MouseRefSWATH) that permits quantification of up to 10,597 proteins (62.2% of the mouse proteome) by SWATH-MS. We exploit MouseRefSWATH to develop an analytical pipeline for species-specific deconvolution of proteomic alterations in human tumour xenografts (XenoSWATH). This method overcomes the challenge of high sequence similarity between mouse and human proteins, facilitating the study of host microenvironment-tumour interactions from ‘bulk tumour’ measurements. We apply the XenoSWATH pipeline to characterize an intraductal xenograft model of breast ductal carcinoma in situ and uncover complex regulation consistent with stromal reprogramming, where the modulation of cell migration pathways is not restricted to tumour cells but also operates in the mouse stroma upon progression to invasive disease. MouseRefSWATH and XenoSWATH open new opportunities for in-depth and reproducible proteomic assessment to address wide-ranging biological questions involving this important model organism.P.H.H. is supported by grants from the Institute of Cancer Research, Breast Cancer Now (2013NovPhD185 and 2014NovPR360) and Cancer Research UK (C36478/ A19281). B.A.H. and R.C.N. are supported by Breast Cancer Now programmatic funding. S.E.A. is supported by grants from Cancer Research UK (CRUK/A19763) and the Medical Research Council (MC_U12266B). F.C. is supported by grants from the Institute of Cancer Research, the Ramon y Cajal Research Programme (RYC2016-20352) and Ministerio de Ciencia, Innovación y Universidades/Agencia Estatal de Investigación/European Regional Development Fund (RTI2018-096778- A-I00). P.-C.H. is supported in part by a Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung project grant (31003A_182470) and a European Research Council Starting Grant (802773-MitoGuide).Company of BiologistsThe Institute of Cancer Research (UK)Breast Cancer Research FoundationMedical Research Council (UK)Ministerio de Economía y Competitividad (España)Ministerio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)European Research CouncilConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2021202120202021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/226604reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC2016-20352info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096778- A-I00RTI2018-096778- A-I00/AEI/10.13039/501100011033http://dx.doi.org/10.1242/dmm.044586Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2266042026-05-22T06:33:51Z |
| dc.title.none.fl_str_mv |
A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts |
| title |
A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts |
| spellingShingle |
A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts Krasny, Lukas Mass spectrometry SWATH-MS Proteomics Mouse Xenografts DCIS Breast cancer |
| title_short |
A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts |
| title_full |
A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts |
| title_fullStr |
A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts |
| title_full_unstemmed |
A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts |
| title_sort |
A mouse SWATH-mass spectrometry reference spectral library enables deconvolution of species-specific proteomic alterations in human tumour xenografts |
| dc.creator.none.fl_str_mv |
Krasny, Lukas Bland, Philip Burns, Jessica Carvalho Lima, Nadia Harrison, Peter T. Pacini, Laura Elms, Mark L. Ning, Jian Garcia Martinez, Victor Yu, Yi-Ru Acton, Sophie E. Ho, Ping-Chih Calvo, Fernando Swain, Amanda Howard, Beatrice A. Natrajan, Rachael C. Huang, Paul H. |
| author |
Krasny, Lukas |
| author_facet |
Krasny, Lukas Bland, Philip Burns, Jessica Carvalho Lima, Nadia Harrison, Peter T. Pacini, Laura Elms, Mark L. Ning, Jian Garcia Martinez, Victor Yu, Yi-Ru Acton, Sophie E. Ho, Ping-Chih Calvo, Fernando Swain, Amanda Howard, Beatrice A. Natrajan, Rachael C. Huang, Paul H. |
| author_role |
author |
| author2 |
Bland, Philip Burns, Jessica Carvalho Lima, Nadia Harrison, Peter T. Pacini, Laura Elms, Mark L. Ning, Jian Garcia Martinez, Victor Yu, Yi-Ru Acton, Sophie E. Ho, Ping-Chih Calvo, Fernando Swain, Amanda Howard, Beatrice A. Natrajan, Rachael C. Huang, Paul H. |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
The Institute of Cancer Research (UK) Breast Cancer Research Foundation Medical Research Council (UK) Ministerio de Economía y Competitividad (España) Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) European Research Council Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] |
| dc.subject.none.fl_str_mv |
Mass spectrometry SWATH-MS Proteomics Mouse Xenografts DCIS Breast cancer |
| topic |
Mass spectrometry SWATH-MS Proteomics Mouse Xenografts DCIS Breast cancer |
| description |
© 2020. Published by The Company of Biologists Ltd. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2021 2021 2021 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article http://purl.org/coar/resource_type/c_6501 Publisher's version info:eu-repo/semantics/publishedVersion |
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article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
http://hdl.handle.net/10261/226604 |
| url |
http://hdl.handle.net/10261/226604 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
#PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RYC2016-20352 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096778- A-I00 RTI2018-096778- A-I00/AEI/10.13039/501100011033 http://dx.doi.org/10.1242/dmm.044586 Sí |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.publisher.none.fl_str_mv |
Company of Biologists |
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Company of Biologists |
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reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC instname:Consejo Superior de Investigaciones Científicas (CSIC) |
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Consejo Superior de Investigaciones Científicas (CSIC) |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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DIGITAL.CSIC. Repositorio Institucional del CSIC |
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