Activity of the hippocampal somatostatinergic system following daily administration of melatonin

If melatonin or its analogs are to be used therapeutically in humans, their chronic effects on responsiveness of melatonin target cells need to be assessed. We have previously demonstrated that acute melatonin treatment regulates the somatostatinergic system in the rat hippocampus. In the present st...

ver descrição completa

Detalhes bibliográficos
Autores: Izquierdo Claros, Rosa María, Boyano Adánez, María del Carmen|||0000-0003-2799-6613, Arilla Ferreiro, Eduardo
Tipo de documento: artigo
Data de publicação:2004
País:España
Recursos:Universidad de Alcalá (UAH)
Repositório:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglês
OAI Identifier:oai:ebuah.uah.es:10017/2259
Acesso em linha:http://hdl.handle.net/10017/2259
https://dx.doi.org/10.1016/j.molbrainres.2004.03.010
Access Level:Acceso aberto
Palavra-chave:Somatostatin receptors
Melatonin
Adenylyl cyclase
G proteins
Hippocampus
Rat
Bioquímica
Ciencia
Biochemistry
Science
id ES_69b4331bd8de41ad7ce5b7464cce89ff
oai_identifier_str oai:ebuah.uah.es:10017/2259
network_acronym_str ES
network_name_str España
repository_id_str
spelling Activity of the hippocampal somatostatinergic system following daily administration of melatoninIzquierdo Claros, Rosa MaríaBoyano Adánez, María del Carmen|||0000-0003-2799-6613Arilla Ferreiro, EduardoSomatostatin receptorsMelatoninAdenylyl cyclaseG proteinsHippocampusRatBioquímicaCienciaBiochemistryScienceIf melatonin or its analogs are to be used therapeutically in humans, their chronic effects on responsiveness of melatonin target cells need to be assessed. We have previously demonstrated that acute melatonin treatment regulates the somatostatinergic system in the rat hippocampus. In the present study, we have investigated the effects of subchronic and chronic daily treatment with melatonin on the somatostatinergic system in the rat hippocampus. Male Wistar rats (200-250 g) were injected with melatonin (25 ¿g/kg body weight, subcutaneously) daily for 4, 7 or 14 days and sacrificed 24 h after the last injection. Melatonin administration for 4 days induced a decrease in the hippocampal somatostatin (SRIF)-like immunoreactivity content as well as a decrease in the number of SRIF receptors and an increase in their apparent affinity. The decreased number of SRIF receptors in the melatonin (4 days)-treated rats was associated with a decreased capacity of SRIF to inhibit both basal and forskolin-stimulated adenylyl cyclase activity. These melatonin-induced effects reversed to control values after 7 or 14 days of treatment. Hippocampal membranes from control and melatonin-treated rats showed similar Gi and Gs activities. Melatonin treatment altered neither the functional Gi activity nor the Gi¿1 or Gi¿2 levels at any of the time periods studied. The present results suggest that chronic exposure to melatonin results in a tolerance of the hippocampus to this hormone.Ministerio de Ciencia y TecnologíaUniversidad de AlcaláElsevier20042004-01-01journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/2259https://dx.doi.org/10.1016/j.molbrainres.2004.03.010reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengMICYT Not available PM99-0129 REGULACIÓN DEL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA DEL CEREBRO DE RATA POR DERIVADOS DE LA PROTEÍNA PRECURSORA BETA-AMILOIDENot available Not available Not availableNot available Not available Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/22592026-06-18T11:13:07Z
dc.title.none.fl_str_mv Activity of the hippocampal somatostatinergic system following daily administration of melatonin
title Activity of the hippocampal somatostatinergic system following daily administration of melatonin
spellingShingle Activity of the hippocampal somatostatinergic system following daily administration of melatonin
Izquierdo Claros, Rosa María
Somatostatin receptors
Melatonin
Adenylyl cyclase
G proteins
Hippocampus
Rat
Bioquímica
Ciencia
Biochemistry
Science
title_short Activity of the hippocampal somatostatinergic system following daily administration of melatonin
title_full Activity of the hippocampal somatostatinergic system following daily administration of melatonin
title_fullStr Activity of the hippocampal somatostatinergic system following daily administration of melatonin
title_full_unstemmed Activity of the hippocampal somatostatinergic system following daily administration of melatonin
title_sort Activity of the hippocampal somatostatinergic system following daily administration of melatonin
dc.creator.none.fl_str_mv Izquierdo Claros, Rosa María
Boyano Adánez, María del Carmen|||0000-0003-2799-6613
Arilla Ferreiro, Eduardo
author Izquierdo Claros, Rosa María
author_facet Izquierdo Claros, Rosa María
Boyano Adánez, María del Carmen|||0000-0003-2799-6613
Arilla Ferreiro, Eduardo
author_role author
author2 Boyano Adánez, María del Carmen|||0000-0003-2799-6613
Arilla Ferreiro, Eduardo
author2_role author
author
dc.subject.none.fl_str_mv Somatostatin receptors
Melatonin
Adenylyl cyclase
G proteins
Hippocampus
Rat
Bioquímica
Ciencia
Biochemistry
Science
topic Somatostatin receptors
Melatonin
Adenylyl cyclase
G proteins
Hippocampus
Rat
Bioquímica
Ciencia
Biochemistry
Science
description If melatonin or its analogs are to be used therapeutically in humans, their chronic effects on responsiveness of melatonin target cells need to be assessed. We have previously demonstrated that acute melatonin treatment regulates the somatostatinergic system in the rat hippocampus. In the present study, we have investigated the effects of subchronic and chronic daily treatment with melatonin on the somatostatinergic system in the rat hippocampus. Male Wistar rats (200-250 g) were injected with melatonin (25 ¿g/kg body weight, subcutaneously) daily for 4, 7 or 14 days and sacrificed 24 h after the last injection. Melatonin administration for 4 days induced a decrease in the hippocampal somatostatin (SRIF)-like immunoreactivity content as well as a decrease in the number of SRIF receptors and an increase in their apparent affinity. The decreased number of SRIF receptors in the melatonin (4 days)-treated rats was associated with a decreased capacity of SRIF to inhibit both basal and forskolin-stimulated adenylyl cyclase activity. These melatonin-induced effects reversed to control values after 7 or 14 days of treatment. Hippocampal membranes from control and melatonin-treated rats showed similar Gi and Gs activities. Melatonin treatment altered neither the functional Gi activity nor the Gi¿1 or Gi¿2 levels at any of the time periods studied. The present results suggest that chronic exposure to melatonin results in a tolerance of the hippocampus to this hormone.
publishDate 2004
dc.date.none.fl_str_mv 2004
2004-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/2259
https://dx.doi.org/10.1016/j.molbrainres.2004.03.010
url http://hdl.handle.net/10017/2259
https://dx.doi.org/10.1016/j.molbrainres.2004.03.010
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv MICYT Not available PM99-0129 REGULACIÓN DEL SISTEMA RECEPTOR-EFECTOR DE LA SOMATOSTATINA DEL CEREBRO DE RATA POR DERIVADOS DE LA PROTEÍNA PRECURSORA BETA-AMILOIDE
Not available Not available Not available
Not available Not available Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410048488243201
score 15,300719

Registros relacionados