Effect of cocoa's theobromine on intestinal microbiota of rats

SCOPE: To establish the role of cocoa theobromine on gut microbiota composition and fermentation products after cocoa consumption in rats. METHODS AND RESULTS: Lewis rats were fed either a standard diet (RF diet), a diet containing 10% cocoa (CC diet) or a diet including 0.25% theobromine (TB diet)...

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Authors: Martín-Peláez, Sandra, Camps i Bossacoma, Mariona, Massot Cladera, Malen, Rigo Adrover, Maria del Mar, Franch i Masferrer, Àngels, Pérez-Cano, Francisco J., Castell, Margarida
Format: article
Status:Versión aceptada para publicación
Publication Date:2017
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/165544
Online Access:https://hdl.handle.net/2445/165544
Access Level:Open access
Keyword:Metagenòmica
Microbiota intestinal
Cacau
Farmacologia
Farmacocinètica
Dieta
Rates (Animals de laboratori)
Metagenomics
Gastrointestinal microbiome
Cocoa
Pharmacology
Pharmacokinetics
Diet
Rats as laboratory animals
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oai_identifier_str oai:recercat.cat:2445/165544
network_acronym_str ES
network_name_str España
repository_id_str
spelling Effect of cocoa's theobromine on intestinal microbiota of ratsMartín-Peláez, SandraCamps i Bossacoma, MarionaMassot Cladera, MalenRigo Adrover, Maria del MarFranch i Masferrer, ÀngelsPérez-Cano, Francisco J.Castell, MargaridaMetagenòmicaMicrobiota intestinalCacauFarmacologiaFarmacocinèticaDietaRates (Animals de laboratori)MetagenomicsGastrointestinal microbiomeCocoaPharmacologyPharmacokineticsDietRats as laboratory animalsSCOPE: To establish the role of cocoa theobromine on gut microbiota composition and fermentation products after cocoa consumption in rats. METHODS AND RESULTS: Lewis rats were fed either a standard diet (RF diet), a diet containing 10% cocoa (CC diet) or a diet including 0.25% theobromine (TB diet) for 15 days. Gut microbiota (fluorescence in situ hybridization coupled to flow cytometry and metagenomics analysis), SCFA and IgA-coated bacteria were analyzed in fecal samples. CC and TB diets induced lower counts of E. coli whereas TB diet led to lower counts of Bifidobacterium spp., Streptococcus spp. and Clostridium histolyticum-C. perfingens group compared to RF diet. Metagenomics analysis also revealed a different microbiota pattern among the studied groups. The SCFA content was higher after both CC and TB diets, which was mainly due to enhanced butyric acid production. Furthermore, both diets decreased the proportion of IgA-coated bacteria. CONCLUSION: Cocoa's theobromine plays a relevant role in some effects related to cocoa intake, such as the lower proportion of IgA-coated bacteria. Moreover, theobromine modifies gut microbiota although other cocoa compounds could also act on intestinal bacteria, attenuating or enhancing the theobromine effects.Wiley-VCH2020202020172020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/165544Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1002/mnfr.201700238Molecular Nutrition & Food Research, 2017, vol. 61, num. 10, p. 1700238https://doi.org/10.1002/mnfr.201700238(c) Wiley-VCH, 2017info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1655442026-05-29T05:05:01Z
dc.title.none.fl_str_mv Effect of cocoa's theobromine on intestinal microbiota of rats
title Effect of cocoa's theobromine on intestinal microbiota of rats
spellingShingle Effect of cocoa's theobromine on intestinal microbiota of rats
Martín-Peláez, Sandra
Metagenòmica
Microbiota intestinal
Cacau
Farmacologia
Farmacocinètica
Dieta
Rates (Animals de laboratori)
Metagenomics
Gastrointestinal microbiome
Cocoa
Pharmacology
Pharmacokinetics
Diet
Rats as laboratory animals
title_short Effect of cocoa's theobromine on intestinal microbiota of rats
title_full Effect of cocoa's theobromine on intestinal microbiota of rats
title_fullStr Effect of cocoa's theobromine on intestinal microbiota of rats
title_full_unstemmed Effect of cocoa's theobromine on intestinal microbiota of rats
title_sort Effect of cocoa's theobromine on intestinal microbiota of rats
dc.creator.none.fl_str_mv Martín-Peláez, Sandra
Camps i Bossacoma, Mariona
Massot Cladera, Malen
Rigo Adrover, Maria del Mar
Franch i Masferrer, Àngels
Pérez-Cano, Francisco J.
Castell, Margarida
author Martín-Peláez, Sandra
author_facet Martín-Peláez, Sandra
Camps i Bossacoma, Mariona
Massot Cladera, Malen
Rigo Adrover, Maria del Mar
Franch i Masferrer, Àngels
Pérez-Cano, Francisco J.
Castell, Margarida
author_role author
author2 Camps i Bossacoma, Mariona
Massot Cladera, Malen
Rigo Adrover, Maria del Mar
Franch i Masferrer, Àngels
Pérez-Cano, Francisco J.
Castell, Margarida
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Metagenòmica
Microbiota intestinal
Cacau
Farmacologia
Farmacocinètica
Dieta
Rates (Animals de laboratori)
Metagenomics
Gastrointestinal microbiome
Cocoa
Pharmacology
Pharmacokinetics
Diet
Rats as laboratory animals
topic Metagenòmica
Microbiota intestinal
Cacau
Farmacologia
Farmacocinètica
Dieta
Rates (Animals de laboratori)
Metagenomics
Gastrointestinal microbiome
Cocoa
Pharmacology
Pharmacokinetics
Diet
Rats as laboratory animals
description SCOPE: To establish the role of cocoa theobromine on gut microbiota composition and fermentation products after cocoa consumption in rats. METHODS AND RESULTS: Lewis rats were fed either a standard diet (RF diet), a diet containing 10% cocoa (CC diet) or a diet including 0.25% theobromine (TB diet) for 15 days. Gut microbiota (fluorescence in situ hybridization coupled to flow cytometry and metagenomics analysis), SCFA and IgA-coated bacteria were analyzed in fecal samples. CC and TB diets induced lower counts of E. coli whereas TB diet led to lower counts of Bifidobacterium spp., Streptococcus spp. and Clostridium histolyticum-C. perfingens group compared to RF diet. Metagenomics analysis also revealed a different microbiota pattern among the studied groups. The SCFA content was higher after both CC and TB diets, which was mainly due to enhanced butyric acid production. Furthermore, both diets decreased the proportion of IgA-coated bacteria. CONCLUSION: Cocoa's theobromine plays a relevant role in some effects related to cocoa intake, such as the lower proportion of IgA-coated bacteria. Moreover, theobromine modifies gut microbiota although other cocoa compounds could also act on intestinal bacteria, attenuating or enhancing the theobromine effects.
publishDate 2017
dc.date.none.fl_str_mv 2017
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/165544
url https://hdl.handle.net/2445/165544
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1002/mnfr.201700238
Molecular Nutrition & Food Research, 2017, vol. 61, num. 10, p. 1700238
https://doi.org/10.1002/mnfr.201700238
dc.rights.none.fl_str_mv (c) Wiley-VCH, 2017
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Wiley-VCH, 2017
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410044031795200
score 15,811543

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