Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin

Electrochemical immunosensors for ceruloplasmin (Cp) are reported for the first time. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp antibodies were compared, using competitive immunoassay with synthesized alkaline phosphatase...

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Detalhes bibliográficos
Autores: Ojeda Fernández, Irene, Moreno Guzmán, María, González Cortés, Araceli, Yáñez-Sedeño Orive, Paloma, Pingarrón Carrazón, José Manuel
Formato: artículo
Fecha de publicación:2013
País:España
Recursos:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/42743
Acesso em linha:https://hdl.handle.net/20.500.14352/42743
Access Level:acceso abierto
Palavra-chave:543
Ceruloplasmin
Electrochemical magnetic immunosensor
Human serum
Química analítica (Química)
2301 Química Analítica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/42743
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repository_id_str
spelling Electrochemical Magnetic Immunosensors for the Determination of CeruloplasminOjeda Fernández, IreneMoreno Guzmán, MaríaGonzález Cortés, AraceliYáñez-Sedeño Orive, PalomaPingarrón Carrazón, José Manuel543CeruloplasminElectrochemical magnetic immunosensorHuman serumQuímica analítica (Química)2301 Química AnalíticaElectrochemical immunosensors for ceruloplasmin (Cp) are reported for the first time. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp antibodies were compared, using competitive immunoassay with synthesized alkaline phosphatase-Cp conjugate. Upon capturing MBs-immunoconjugates onto screen-printed carbon electrodes, quantification of Cp was accomplished by DPV measurement of 1-naphthol generated after 1-naphthylphosphate addition. Linear ranges of calibration curves and detection limits were 0.1–1000 µg/mL and 0.040 µg/mL (Protein A-MBs), and 0.025–20 µg/mL and 0.018 µg/mL (Strept-MBs). Good results were obtained in the determination of Cp in spiked human serum samples.Wiley-VCHUniversidad Complutense de Madrid20132013-01-0120132013-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/42743reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/427432026-06-02T12:44:21Z
dc.title.none.fl_str_mv Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin
title Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin
spellingShingle Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin
Ojeda Fernández, Irene
543
Ceruloplasmin
Electrochemical magnetic immunosensor
Human serum
Química analítica (Química)
2301 Química Analítica
title_short Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin
title_full Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin
title_fullStr Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin
title_full_unstemmed Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin
title_sort Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin
dc.creator.none.fl_str_mv Ojeda Fernández, Irene
Moreno Guzmán, María
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author Ojeda Fernández, Irene
author_facet Ojeda Fernández, Irene
Moreno Guzmán, María
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author_role author
author2 Moreno Guzmán, María
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Ceruloplasmin
Electrochemical magnetic immunosensor
Human serum
Química analítica (Química)
2301 Química Analítica
topic 543
Ceruloplasmin
Electrochemical magnetic immunosensor
Human serum
Química analítica (Química)
2301 Química Analítica
description Electrochemical immunosensors for ceruloplasmin (Cp) are reported for the first time. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp antibodies were compared, using competitive immunoassay with synthesized alkaline phosphatase-Cp conjugate. Upon capturing MBs-immunoconjugates onto screen-printed carbon electrodes, quantification of Cp was accomplished by DPV measurement of 1-naphthol generated after 1-naphthylphosphate addition. Linear ranges of calibration curves and detection limits were 0.1–1000 µg/mL and 0.040 µg/mL (Protein A-MBs), and 0.025–20 µg/mL and 0.018 µg/mL (Strept-MBs). Good results were obtained in the determination of Cp in spiked human serum samples.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01
2013
2013-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/42743
url https://hdl.handle.net/20.500.14352/42743
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-VCH
publisher.none.fl_str_mv Wiley-VCH
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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