Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave

In fission yeast, MBF-dependent transcription is inactivated at the end of S phase through a negative feedback loop that involves the co-repressors, Yox1 and Nrm1. Although this repression system is well known, the molecular mechanisms involved in MBF activation remain largely unknown. Compacted chr...

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Authors: González Medina, Alberto, 1990-, Hidalgo Hernando, Elena, Ayté del Olmo, José
Format: article
Status:Published version
Publication Date:2019
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/42752
Online Access:http://hdl.handle.net/10230/42752
http://dx.doi.org/10.1093/nar/gkz561
Access Level:Open access
Keyword:Gene regulation
Chromatin and epigenetics
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spelling Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional waveGonzález Medina, Alberto, 1990-Hidalgo Hernando, ElenaAyté del Olmo, JoséGene regulationChromatin and epigeneticsIn fission yeast, MBF-dependent transcription is inactivated at the end of S phase through a negative feedback loop that involves the co-repressors, Yox1 and Nrm1. Although this repression system is well known, the molecular mechanisms involved in MBF activation remain largely unknown. Compacted chromatin constitutes a barrier to activators accessing promoters. Here, we show that chromatin regulation plays a key role in activating MBF-dependent transcription. Gcn5, a part of the SAGA complex, binds to MBF-regulated promoters through the MBF co-activator Rep2 in a cell cycle-dependent manner and in a reverse correlation to the binding of the MBF co-repressors, Nrm1 or Yox1. We propose that the co-repressors function as physical barriers to SAGA recruitment onto MBF promoters. We also show that Gcn5 acetylates specific lysine residues on histone H3 in a cell cycle-regulated manner. Furthermore, either in a gcn5 mutant or in a strain in which histone H3 is kept in an unacetylated form, MBF-dependent transcription is downregulated. In summary, Gcn5 is required for the full activation and correct timing of MBF-regulated gene transcription.Spanish Ministerio de Economia y Competitividad, PLAN E, and Feder [BFU2015-66347, PGC2018-097248-B-I00]; MEIONet [BFU2015-71786-REDT]; Unidad de Excelencia Maria de Maeztu [MDM-2014-0370]; ICREA Academia Award (Generalitat de Catalunya) (to E.H.). Funding for open access charge: Spanish Ministerio de Economia y Competitividad [BFU2015-66347, PGC2018-097248-B-I00].Oxford University Press201920192019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/42752http://dx.doi.org/10.1093/nar/gkz561reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésNucleic Acids Research. 2019;47(16):8439-51info:eu-repo/grantAgreement/ES/1PE/BFU2015-66347info:eu-repo/grantAgreement/ES/2PE/PGC2018-097248-B-I00info:eu-repo/grantAgreement/ES/1PE/BFU2015-71786-REDT© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.comhttp://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/427522026-05-29T05:05:01Z
dc.title.none.fl_str_mv Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave
title Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave
spellingShingle Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave
González Medina, Alberto, 1990-
Gene regulation
Chromatin and epigenetics
title_short Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave
title_full Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave
title_fullStr Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave
title_full_unstemmed Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave
title_sort Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave
dc.creator.none.fl_str_mv González Medina, Alberto, 1990-
Hidalgo Hernando, Elena
Ayté del Olmo, José
author González Medina, Alberto, 1990-
author_facet González Medina, Alberto, 1990-
Hidalgo Hernando, Elena
Ayté del Olmo, José
author_role author
author2 Hidalgo Hernando, Elena
Ayté del Olmo, José
author2_role author
author
dc.subject.none.fl_str_mv Gene regulation
Chromatin and epigenetics
topic Gene regulation
Chromatin and epigenetics
description In fission yeast, MBF-dependent transcription is inactivated at the end of S phase through a negative feedback loop that involves the co-repressors, Yox1 and Nrm1. Although this repression system is well known, the molecular mechanisms involved in MBF activation remain largely unknown. Compacted chromatin constitutes a barrier to activators accessing promoters. Here, we show that chromatin regulation plays a key role in activating MBF-dependent transcription. Gcn5, a part of the SAGA complex, binds to MBF-regulated promoters through the MBF co-activator Rep2 in a cell cycle-dependent manner and in a reverse correlation to the binding of the MBF co-repressors, Nrm1 or Yox1. We propose that the co-repressors function as physical barriers to SAGA recruitment onto MBF promoters. We also show that Gcn5 acetylates specific lysine residues on histone H3 in a cell cycle-regulated manner. Furthermore, either in a gcn5 mutant or in a strain in which histone H3 is kept in an unacetylated form, MBF-dependent transcription is downregulated. In summary, Gcn5 is required for the full activation and correct timing of MBF-regulated gene transcription.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/42752
http://dx.doi.org/10.1093/nar/gkz561
url http://hdl.handle.net/10230/42752
http://dx.doi.org/10.1093/nar/gkz561
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Nucleic Acids Research. 2019;47(16):8439-51
info:eu-repo/grantAgreement/ES/1PE/BFU2015-66347
info:eu-repo/grantAgreement/ES/2PE/PGC2018-097248-B-I00
info:eu-repo/grantAgreement/ES/1PE/BFU2015-71786-REDT
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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