Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows

Colostrum is rich in membranous vesicles of endocytic origin named exosomes, with proteins, lipids, RNA, and/or DNA cargos which can play different roles in physiological processes. Like other colostrum bioactive compounds, exosomes could be also influenced by individual characteristics. The objecti...

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Detalles Bibliográficos
Autores: Terre, Marta, Alomar, Oscar, Garcia-Fruitos, Elena, Fàbregas, Francesc, Bach, Alex
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
Repositorio:IRTA Pubpro. Open Digital Archive
OAI Identifier:oai:repositori.irta.cat:20.500.12327/3416
Acceso en línea:http://hdl.handle.net/20.500.12327/3416
https://doi.org/10.3389/fvets.2024.1463342
Access Level:acceso abierto
Palabra clave:636
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spelling Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cowsTerre, MartaAlomar, OscarGarcia-Fruitos, ElenaFàbregas, FrancescBach, Alex636Colostrum is rich in membranous vesicles of endocytic origin named exosomes, with proteins, lipids, RNA, and/or DNA cargos which can play different roles in physiological processes. Like other colostrum bioactive compounds, exosomes could be also influenced by individual characteristics. The objective of the study was to characterize miRNA cargo of colostrum exosomes from primiparous and multiparous cows in different farms. Twenty-seven colostrum samples of clinically healthy Holstein cows (11 primiparous and 16 multiparous) from 3 different farms were obtained and frozen. After thawing, exosomes were isolated following an ultracentrifugation protocol, and characterized morphologically. Particle size distribution and western immunoblotting were also analyzMaed. After RNA extraction, miRNAs were sequenced and analyzed to assess potential differences in profiles between primiparous and multiparous cows from different farms. Fourteen miRNA were upregulated and 11 miRNAs downregulated in primiparous compared with multiparous cows. Most of the miRNA differences between primiparous and multiparous cows regulate the gene expression of factors involved in mammary gland development and differentiation, and lipogenesis. In addition, miRNAs from one of the farms showed 8 miRNAs downregulated and 12 upregulated compared with the other 2 farms, independently of parity. Differences in miRNA between farms were mainly associated with immune and inflammatory-related genes. In conclusion, miRNA cargos of bovine colostrum exosomes differ in primiparous and multiparous cows, and some on-farm practices might also determine the content and activity of miRNA in colostrum exosomes.The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported by the Spanish Ministry of Economy and Competitivity and FEDER (PGC2018-098847-B-100). IRTA thanks the funding support of the CERCA Program and the Consolidated Research Groups of Sustainable Animal Husbandry (ref. 2021 SGR 01552), both from the Generalitat de Catalunya.info:eu-repo/semantics/publishedVersionFrontiers MediaProducció AnimalProducció de Remugants202420242024info:eu-repo/semantics/article8application/pdfhttp://hdl.handle.net/20.500.12327/3416https://doi.org/10.3389/fvets.2024.1463342reponame:IRTA Pubpro. Open Digital Archiveinstname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)InglésFrontiers in Veterinary ScienceMICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/PGC2018-098847-B-I00/ES/EL PAPEL DE LOS EXOSOMAS DEL CALOSTRO BOVINO SOBRE LA IMPRIMACION METABOLICA EN TERNERAS/FEDER/ / /EU/ /Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.irta.cat:20.500.12327/34162026-06-16T08:51:17Z
dc.title.none.fl_str_mv Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows
title Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows
spellingShingle Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows
Terre, Marta
636
title_short Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows
title_full Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows
title_fullStr Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows
title_full_unstemmed Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows
title_sort Micro RNA profiles in colostrum exosomes obtained from primiparous or multiparous dairy cows
dc.creator.none.fl_str_mv Terre, Marta
Alomar, Oscar
Garcia-Fruitos, Elena
Fàbregas, Francesc
Bach, Alex
author Terre, Marta
author_facet Terre, Marta
Alomar, Oscar
Garcia-Fruitos, Elena
Fàbregas, Francesc
Bach, Alex
author_role author
author2 Alomar, Oscar
Garcia-Fruitos, Elena
Fàbregas, Francesc
Bach, Alex
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Producció Animal
Producció de Remugants
dc.subject.none.fl_str_mv 636
topic 636
description Colostrum is rich in membranous vesicles of endocytic origin named exosomes, with proteins, lipids, RNA, and/or DNA cargos which can play different roles in physiological processes. Like other colostrum bioactive compounds, exosomes could be also influenced by individual characteristics. The objective of the study was to characterize miRNA cargo of colostrum exosomes from primiparous and multiparous cows in different farms. Twenty-seven colostrum samples of clinically healthy Holstein cows (11 primiparous and 16 multiparous) from 3 different farms were obtained and frozen. After thawing, exosomes were isolated following an ultracentrifugation protocol, and characterized morphologically. Particle size distribution and western immunoblotting were also analyzMaed. After RNA extraction, miRNAs were sequenced and analyzed to assess potential differences in profiles between primiparous and multiparous cows from different farms. Fourteen miRNA were upregulated and 11 miRNAs downregulated in primiparous compared with multiparous cows. Most of the miRNA differences between primiparous and multiparous cows regulate the gene expression of factors involved in mammary gland development and differentiation, and lipogenesis. In addition, miRNAs from one of the farms showed 8 miRNAs downregulated and 12 upregulated compared with the other 2 farms, independently of parity. Differences in miRNA between farms were mainly associated with immune and inflammatory-related genes. In conclusion, miRNA cargos of bovine colostrum exosomes differ in primiparous and multiparous cows, and some on-farm practices might also determine the content and activity of miRNA in colostrum exosomes.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12327/3416
https://doi.org/10.3389/fvets.2024.1463342
url http://hdl.handle.net/20.500.12327/3416
https://doi.org/10.3389/fvets.2024.1463342
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Frontiers in Veterinary Science
MICIU/Programa Estatal de generación del conocimiento y fortalecimiento científico y tecnológico del sistema I+D+I/PGC2018-098847-B-I00/ES/EL PAPEL DE LOS EXOSOMAS DEL CALOSTRO BOVINO SOBRE LA IMPRIMACION METABOLICA EN TERNERAS/
FEDER/ / /EU/ /
dc.rights.none.fl_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 8
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:IRTA Pubpro. Open Digital Archive
instname:Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
instname_str Institut de Recerca i Tecnologia Agroalimentàries (IRTA)
reponame_str IRTA Pubpro. Open Digital Archive
collection IRTA Pubpro. Open Digital Archive
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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