Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating

The design and engineering of antibacterial materials are key for preventing bacterial adherence and proliferation in biomedical and household instruments. Silver nanoparticles (AgNPs) and chitosan (CHI) are broad-spectrum antibacterial materials with different properties whose combined application...

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Authors: Naveas, Nelson, Pulido, Ruth, Torres-Costa, Vicente, Agulló Rueda, F., Santibáñez, Mauricio, Malano, Francisco, Recio-Sánchez, Gonzalo, Garrido-Miranda, Karla A., Manso-Silván, Miguel, Hernández-Montelongo, Jacobo
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/330392
Online Access:http://hdl.handle.net/10261/330392
Access Level:Open access
Keyword:Antibacterial films
Carboxymethylcellulose
Chitosan
Silver nanoparticles
Nanoporous silicon
Composite material
Layer-by-layer
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spelling Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin CoatingNaveas, NelsonPulido, RuthTorres-Costa, VicenteAgulló Rueda, F.Santibáñez, MauricioMalano, FranciscoRecio-Sánchez, GonzaloGarrido-Miranda, Karla A.Manso-Silván, MiguelHernández-Montelongo, JacoboAntibacterial filmsCarboxymethylcelluloseChitosanSilver nanoparticlesNanoporous siliconComposite materialLayer-by-layerThe design and engineering of antibacterial materials are key for preventing bacterial adherence and proliferation in biomedical and household instruments. Silver nanoparticles (AgNPs) and chitosan (CHI) are broad-spectrum antibacterial materials with different properties whose combined application is currently under optimization. This study proposes the formation of antibacterial films with AgNPs embedded in carboxymethylcellulose/chitosan multilayers by the layer-by-layer (LbL) method. The films were deposited onto nanoporous silicon (nPSi), an ideal platform for bioengineering applications due to its biocompatibility, biodegradability, and bioresorbability. We focused on two alternative multilayer deposition processes: cyclic dip coating (CDC) and cyclic spin coating (CSC). The physicochemical properties of the films were the subject of microscopic, microstructural, and surface–interface analyses. The antibacterial activity of each film was investigated against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria strains as model microorganisms. According to the findings, the CDC technique produced multilayer films with higher antibacterial activity for both bacteria compared to the CSC method. Bacteria adhesion inhibition was observed from only three cycles. The developed AgNPs–multilayer composite film offers advantageous antibacterial properties for biomedical applications.his work was financially supported by Fondo Nacional de Desarrollo Científico Tecnológico FONDECYT—Chile (grant number 11180395), CONICYT PFCHA/DOCTORADO/2017-21172001 (Nelson Naveas), PFCHA/DOCTORADO/2015-21151648 (Ruth Pulido), and Vicerrectoría de Investigación y Doctorados de la Universidad San Sebastian—VRIDPuente21/03 project. The work was partially funded by project USAMPSA PID2020-112770RB-C22 from Ministerio de Ciencia e Innovación de España.Peer reviewedMultidisciplinary Digital Publishing InstituteFondo Nacional de Desarrollo Científico y Tecnológico (Chile)Comisión Nacional de Investigación Científica y Tecnológica (Chile)Universidad San SebastiánMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2023202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/330392reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112770RB-C22https://doi.org/10.3390/ijms241310595Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3303922026-05-22T06:33:51Z
dc.title.none.fl_str_mv Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
title Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
spellingShingle Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
Naveas, Nelson
Antibacterial films
Carboxymethylcellulose
Chitosan
Silver nanoparticles
Nanoporous silicon
Composite material
Layer-by-layer
title_short Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
title_full Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
title_fullStr Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
title_full_unstemmed Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
title_sort Antibacterial Films of Silver Nanoparticles Embedded into Carboxymethylcellulose/Chitosan Multilayers on Nanoporous Silicon: A Layer-by-Layer Assembly Approach Comparing Dip and Spin Coating
dc.creator.none.fl_str_mv Naveas, Nelson
Pulido, Ruth
Torres-Costa, Vicente
Agulló Rueda, F.
Santibáñez, Mauricio
Malano, Francisco
Recio-Sánchez, Gonzalo
Garrido-Miranda, Karla A.
Manso-Silván, Miguel
Hernández-Montelongo, Jacobo
author Naveas, Nelson
author_facet Naveas, Nelson
Pulido, Ruth
Torres-Costa, Vicente
Agulló Rueda, F.
Santibáñez, Mauricio
Malano, Francisco
Recio-Sánchez, Gonzalo
Garrido-Miranda, Karla A.
Manso-Silván, Miguel
Hernández-Montelongo, Jacobo
author_role author
author2 Pulido, Ruth
Torres-Costa, Vicente
Agulló Rueda, F.
Santibáñez, Mauricio
Malano, Francisco
Recio-Sánchez, Gonzalo
Garrido-Miranda, Karla A.
Manso-Silván, Miguel
Hernández-Montelongo, Jacobo
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fondo Nacional de Desarrollo Científico y Tecnológico (Chile)
Comisión Nacional de Investigación Científica y Tecnológica (Chile)
Universidad San Sebastián
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Antibacterial films
Carboxymethylcellulose
Chitosan
Silver nanoparticles
Nanoporous silicon
Composite material
Layer-by-layer
topic Antibacterial films
Carboxymethylcellulose
Chitosan
Silver nanoparticles
Nanoporous silicon
Composite material
Layer-by-layer
description The design and engineering of antibacterial materials are key for preventing bacterial adherence and proliferation in biomedical and household instruments. Silver nanoparticles (AgNPs) and chitosan (CHI) are broad-spectrum antibacterial materials with different properties whose combined application is currently under optimization. This study proposes the formation of antibacterial films with AgNPs embedded in carboxymethylcellulose/chitosan multilayers by the layer-by-layer (LbL) method. The films were deposited onto nanoporous silicon (nPSi), an ideal platform for bioengineering applications due to its biocompatibility, biodegradability, and bioresorbability. We focused on two alternative multilayer deposition processes: cyclic dip coating (CDC) and cyclic spin coating (CSC). The physicochemical properties of the films were the subject of microscopic, microstructural, and surface–interface analyses. The antibacterial activity of each film was investigated against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) bacteria strains as model microorganisms. According to the findings, the CDC technique produced multilayer films with higher antibacterial activity for both bacteria compared to the CSC method. Bacteria adhesion inhibition was observed from only three cycles. The developed AgNPs–multilayer composite film offers advantageous antibacterial properties for biomedical applications.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/330392
url http://hdl.handle.net/10261/330392
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-112770RB-C22
https://doi.org/10.3390/ijms241310595
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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