A direct, efficient method for the preparation of siRNAs containing ribo like North [3.1.0]hexane pseudosugars

An efficient method for the preparation of siRNAs modified with ribo-like North bicyclo[3.1.0]hexane pseudosugars is described. The combined use of 2'-O-(2-cyanoethoxymethyl) (CEM) and 2'-O-TBDMS protection was successfully employed for RNA synthesis with the added advantage that both grou...

Descripción completa

Detalles Bibliográficos
Autores: Terrazas Martínez, Montserrat, Aviñó Andrés, Anna, Siddiqui, Maqbool A., Marquez, Victor E., Eritja i Casadellà, Ramon
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2011
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/179783
Acceso en línea:https://hdl.handle.net/2445/179783
Access Level:acceso abierto
Palabra clave:RNA
Nucleòsids
Estructura molecular
Nucleosides
Molecular structure
id ES_68be0db2d3f91b889a2f0f7c6df9718a
oai_identifier_str oai:recercat.cat:2445/179783
network_acronym_str ES
network_name_str España
repository_id_str
spelling A direct, efficient method for the preparation of siRNAs containing ribo like North [3.1.0]hexane pseudosugarsTerrazas Martínez, MontserratAviñó Andrés, AnnaSiddiqui, Maqbool A.Marquez, Victor E.Eritja i Casadellà, RamonRNANucleòsidsEstructura molecularRNANucleosidesMolecular structureAn efficient method for the preparation of siRNAs modified with ribo-like North bicyclo[3.1.0]hexane pseudosugars is described. The combined use of 2'-O-(2-cyanoethoxymethyl) (CEM) and 2'-O-TBDMS protection was successfully employed for RNA synthesis with the added advantage that both groups were efficiently removed in a single step. The resulting North ribo-methanocarba-modified siRNAs are compatible with the intracellular RNAi machinery and can mediate specific degradation of target mRNA.American Chemical Society2021202120112021info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion4 p.application/pdfhttps://hdl.handle.net/2445/179783Articles publicats en revistes (Química Inorgànica i Orgànica)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésVersió postprint del document publicat a: https://doi.org/10.1021/ol200909jOrganic Letters, 2011, vol. 13, num. 11, p. 2888-2891https://doi.org/10.1021/ol200909j(c) American Chemical Society , 2011info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1797832026-05-29T05:05:01Z
dc.title.none.fl_str_mv A direct, efficient method for the preparation of siRNAs containing ribo like North [3.1.0]hexane pseudosugars
title A direct, efficient method for the preparation of siRNAs containing ribo like North [3.1.0]hexane pseudosugars
spellingShingle A direct, efficient method for the preparation of siRNAs containing ribo like North [3.1.0]hexane pseudosugars
Terrazas Martínez, Montserrat
RNA
Nucleòsids
Estructura molecular
RNA
Nucleosides
Molecular structure
title_short A direct, efficient method for the preparation of siRNAs containing ribo like North [3.1.0]hexane pseudosugars
title_full A direct, efficient method for the preparation of siRNAs containing ribo like North [3.1.0]hexane pseudosugars
title_fullStr A direct, efficient method for the preparation of siRNAs containing ribo like North [3.1.0]hexane pseudosugars
title_full_unstemmed A direct, efficient method for the preparation of siRNAs containing ribo like North [3.1.0]hexane pseudosugars
title_sort A direct, efficient method for the preparation of siRNAs containing ribo like North [3.1.0]hexane pseudosugars
dc.creator.none.fl_str_mv Terrazas Martínez, Montserrat
Aviñó Andrés, Anna
Siddiqui, Maqbool A.
Marquez, Victor E.
Eritja i Casadellà, Ramon
author Terrazas Martínez, Montserrat
author_facet Terrazas Martínez, Montserrat
Aviñó Andrés, Anna
Siddiqui, Maqbool A.
Marquez, Victor E.
Eritja i Casadellà, Ramon
author_role author
author2 Aviñó Andrés, Anna
Siddiqui, Maqbool A.
Marquez, Victor E.
Eritja i Casadellà, Ramon
author2_role author
author
author
author
dc.subject.none.fl_str_mv RNA
Nucleòsids
Estructura molecular
RNA
Nucleosides
Molecular structure
topic RNA
Nucleòsids
Estructura molecular
RNA
Nucleosides
Molecular structure
description An efficient method for the preparation of siRNAs modified with ribo-like North bicyclo[3.1.0]hexane pseudosugars is described. The combined use of 2'-O-(2-cyanoethoxymethyl) (CEM) and 2'-O-TBDMS protection was successfully employed for RNA synthesis with the added advantage that both groups were efficiently removed in a single step. The resulting North ribo-methanocarba-modified siRNAs are compatible with the intracellular RNAi machinery and can mediate specific degradation of target mRNA.
publishDate 2011
dc.date.none.fl_str_mv 2011
2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/179783
url https://hdl.handle.net/2445/179783
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1021/ol200909j
Organic Letters, 2011, vol. 13, num. 11, p. 2888-2891
https://doi.org/10.1021/ol200909j
dc.rights.none.fl_str_mv (c) American Chemical Society , 2011
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Chemical Society , 2011
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 4 p.
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv Articles publicats en revistes (Química Inorgànica i Orgànica)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869409981705486336
score 15,811543

Similares en LA Referencia