Cytochrome c6 is the main respiratory and photosynthetic soluble electron donor in heterocysts of the cyanobacterium Anabaena sp. PCC 7120

Cytochrome c6 is a soluble electron carrier, present in all known cyanobacteria, that has been replaced by plastocyanin in plants. Despite their high structural differences, both proteins have been reported to be isofunctional in cyanobacteria and green algae, acting as alternative electron carriers...

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Autores: Torrado Maya, Alejandro, Ramírez Moncayo, Carmen, Navarro Carruesco, José Antonio, Mariscal Romero, Vicente, Molina Heredia, Fernando Publio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/130314
Acceso en línea:https://hdl.handle.net/11441/130314
https://doi.org/10.1016/j.bbabio.2018.11.009
Access Level:acceso abierto
Palabra clave:Cyanobacteria
Cytochrome c oxidase
Cytochrome c6
Heterocyst
Photosystem I
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spelling Cytochrome c6 is the main respiratory and photosynthetic soluble electron donor in heterocysts of the cyanobacterium Anabaena sp. PCC 7120Torrado Maya, AlejandroRamírez Moncayo, CarmenNavarro Carruesco, José AntonioMariscal Romero, VicenteMolina Heredia, Fernando PublioCyanobacteriaCytochrome c oxidaseCytochrome c6HeterocystPhotosystem ICytochrome c6 is a soluble electron carrier, present in all known cyanobacteria, that has been replaced by plastocyanin in plants. Despite their high structural differences, both proteins have been reported to be isofunctional in cyanobacteria and green algae, acting as alternative electron carriers from the cytochrome b6-f complex to photosystem I or terminal oxidases. We have investigated the subcellular localization of both cytochrome c6 and plastocyanin in the heterocyst-forming cyanobacterium Anabaena sp. PCC 7120 grown in the presence of combined nitrogen and under diazotrophic conditions. Our studies conclude that cytochrome c6 is expressed at significant levels in heterocysts, even in the presence of copper, condition in which it is strongly repressed in vegetative cells. However, the copper-dependent regulation of plastocyanin is not altered in heterocysts. In addition, in heterocysts, cytochrome c6 has shown to be the main soluble electron carrier to cytochrome c oxidase-2 in respiration. A cytochrome c6 deletion mutant is unable to grow under diazotrophic conditions in the presence of copper, suggesting that cytochrome c6 plays an essential role in the physiology of heterocysts that cannot be covered by plastocyanin.Fundación de Investigación de la Universidad de Sevilla FIUS05710000Ministerio de Economía y Competitividad BIO2015-64169-PJunta de Andalucía PAIDI BIO-022ElsevierBioquímica Vegetal y Biología Molecular2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/130314https://doi.org/10.1016/j.bbabio.2018.11.009reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésBiochimica et Biophysica Acta - Bioenergetics, 1860 (1), 60-68.FIUS05710000BIO2015-64169-PPAIDI BIO-022https://doi.org/10.1016/j.bbabio.2018.11.009info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1303142026-06-17T12:51:07Z
dc.title.none.fl_str_mv Cytochrome c6 is the main respiratory and photosynthetic soluble electron donor in heterocysts of the cyanobacterium Anabaena sp. PCC 7120
title Cytochrome c6 is the main respiratory and photosynthetic soluble electron donor in heterocysts of the cyanobacterium Anabaena sp. PCC 7120
spellingShingle Cytochrome c6 is the main respiratory and photosynthetic soluble electron donor in heterocysts of the cyanobacterium Anabaena sp. PCC 7120
Torrado Maya, Alejandro
Cyanobacteria
Cytochrome c oxidase
Cytochrome c6
Heterocyst
Photosystem I
title_short Cytochrome c6 is the main respiratory and photosynthetic soluble electron donor in heterocysts of the cyanobacterium Anabaena sp. PCC 7120
title_full Cytochrome c6 is the main respiratory and photosynthetic soluble electron donor in heterocysts of the cyanobacterium Anabaena sp. PCC 7120
title_fullStr Cytochrome c6 is the main respiratory and photosynthetic soluble electron donor in heterocysts of the cyanobacterium Anabaena sp. PCC 7120
title_full_unstemmed Cytochrome c6 is the main respiratory and photosynthetic soluble electron donor in heterocysts of the cyanobacterium Anabaena sp. PCC 7120
title_sort Cytochrome c6 is the main respiratory and photosynthetic soluble electron donor in heterocysts of the cyanobacterium Anabaena sp. PCC 7120
dc.creator.none.fl_str_mv Torrado Maya, Alejandro
Ramírez Moncayo, Carmen
Navarro Carruesco, José Antonio
Mariscal Romero, Vicente
Molina Heredia, Fernando Publio
author Torrado Maya, Alejandro
author_facet Torrado Maya, Alejandro
Ramírez Moncayo, Carmen
Navarro Carruesco, José Antonio
Mariscal Romero, Vicente
Molina Heredia, Fernando Publio
author_role author
author2 Ramírez Moncayo, Carmen
Navarro Carruesco, José Antonio
Mariscal Romero, Vicente
Molina Heredia, Fernando Publio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
dc.subject.none.fl_str_mv Cyanobacteria
Cytochrome c oxidase
Cytochrome c6
Heterocyst
Photosystem I
topic Cyanobacteria
Cytochrome c oxidase
Cytochrome c6
Heterocyst
Photosystem I
description Cytochrome c6 is a soluble electron carrier, present in all known cyanobacteria, that has been replaced by plastocyanin in plants. Despite their high structural differences, both proteins have been reported to be isofunctional in cyanobacteria and green algae, acting as alternative electron carriers from the cytochrome b6-f complex to photosystem I or terminal oxidases. We have investigated the subcellular localization of both cytochrome c6 and plastocyanin in the heterocyst-forming cyanobacterium Anabaena sp. PCC 7120 grown in the presence of combined nitrogen and under diazotrophic conditions. Our studies conclude that cytochrome c6 is expressed at significant levels in heterocysts, even in the presence of copper, condition in which it is strongly repressed in vegetative cells. However, the copper-dependent regulation of plastocyanin is not altered in heterocysts. In addition, in heterocysts, cytochrome c6 has shown to be the main soluble electron carrier to cytochrome c oxidase-2 in respiration. A cytochrome c6 deletion mutant is unable to grow under diazotrophic conditions in the presence of copper, suggesting that cytochrome c6 plays an essential role in the physiology of heterocysts that cannot be covered by plastocyanin.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/130314
https://doi.org/10.1016/j.bbabio.2018.11.009
url https://hdl.handle.net/11441/130314
https://doi.org/10.1016/j.bbabio.2018.11.009
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Biochimica et Biophysica Acta - Bioenergetics, 1860 (1), 60-68.
FIUS05710000
BIO2015-64169-P
PAIDI BIO-022
https://doi.org/10.1016/j.bbabio.2018.11.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
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