P53 in Penile Squamous Cell Carcinoma: A Pattern-Based Immunohistochemical Framework with Molecular Correlation

Simple Summary: Penile squamous cell carcinomas harbouring mutations of TP53 have an increased risk of lymph node metastases and an impaired prognosis, but the mutational analysis of the TP53 gene is not available in many pathology laboratories. Although p53 immunohistochemistry (IHC) has been propo...

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Autores: Trias, I, Saco, A, Marimon, L, del Campo, R, Manzotti, C, Ordi, O, del Pino, M, Perez, FM, Vega, N, Alos, S, Martinez, A, Rodriguez-Carunchio, L, Reig, O, Jares, P, Teixido, C, Ajami, T, Corral-Molina, JM, Algaba, F, Ribal, MJ, Ribera-Cortada, I, Rakislova, N
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
Repositorio:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
OAI Identifier:oai:iibsantpau.fundanetsuite.com:p16449
Acceso en línea:https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=16449
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85160626389&doi=10.3390%2fcancers15102719&partnerID=40&md5=608b28fd92daa6805ccf07bb73e1a7dd
Access Level:acceso abierto
Palabra clave:p53 immunohistochemistry
TP53 mutations
penile squamous cell carcinoma
surrogate marker
pattern-based framework
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spelling P53 in Penile Squamous Cell Carcinoma: A Pattern-Based Immunohistochemical Framework with Molecular CorrelationTrias, ISaco, AMarimon, Ldel Campo, RManzotti, COrdi, Odel Pino, MPerez, FMVega, NAlos, SMartinez, ARodriguez-Carunchio, LReig, OJares, PTeixido, CAjami, TCorral-Molina, JMAlgaba, FRibal, MJRibera-Cortada, IRakislova, Np53 immunohistochemistryTP53 mutationspenile squamous cell carcinomasurrogate markerpattern-based frameworkSimple Summary: Penile squamous cell carcinomas harbouring mutations of TP53 have an increased risk of lymph node metastases and an impaired prognosis, but the mutational analysis of the TP53 gene is not available in many pathology laboratories. Although p53 immunohistochemistry (IHC) has been proposed as an alternative to the molecular analysis, the current method of evaluation of p53 IHC has many inaccuracies. The aim of our study was to determine, in a series of 40 penile tumours, if a recently described pattern-based framework of p53 IHC evaluation correlates better than the classical method with the TP53 mutational status. Our results show that the new method has a very good correlation with TP53 mutations (95% sensitivity; 92% specificity), higher than that of the classical method, and can be considered as a reliable surrogate of the TP53 mutational status. This new framework can help clinicians to better define risk groups and refine treatment strategies. p53 immunohistochemistry (IHC) has been proposed as a surrogate for TP53 mutations in penile squamous cell carcinomas (PSCC). We aimed to evaluate the performance of a pattern-based evaluation of p53 IHC in PSCC. Human papilloma virus (HPV) DNA testing, p16 and p53 IHC, and whole exome sequencing were performed in a series of 40 PSCC. p53 IHC was evaluated following a pattern-based framework and conventional p53 IHC evaluation. Out of 40 PSCC, 12 (30.0%) were HPV-associated, and 28 (70.0%) were HPV-independent. The agreement between the p53 IHC pattern-based evaluation and TP53 mutational status was almost perfect (k = 0.85). The sensitivity and accuracy of the pattern-based framework for identifying TP53 mutations were 95.5% and 92.5%, respectively, which were higher than the values of conventional p53 IHC interpretation (54.5% and 70.0%, respectively), whereas the specificity was the same (88.9%). In conclusions, the pattern-based framework improves the accuracy of detecting TP53 mutations in PSCC compared to the classical p53 IHC evaluation.MDPI2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=16449https://www.scopus.com/inward/record.uri?eid=2-s2.0-85160626389&doi=10.3390%2fcancers15102719&partnerID=40&md5=608b28fd92daa6805ccf07bb73e1a7ddCancersISSN: 20726694reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pauinstname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)Inglésinfo:eu-repo/semantics/openAccessoai:iibsantpau.fundanetsuite.com:p164492026-06-14T12:41:47Z
dc.title.none.fl_str_mv P53 in Penile Squamous Cell Carcinoma: A Pattern-Based Immunohistochemical Framework with Molecular Correlation
title P53 in Penile Squamous Cell Carcinoma: A Pattern-Based Immunohistochemical Framework with Molecular Correlation
spellingShingle P53 in Penile Squamous Cell Carcinoma: A Pattern-Based Immunohistochemical Framework with Molecular Correlation
Trias, I
p53 immunohistochemistry
TP53 mutations
penile squamous cell carcinoma
surrogate marker
pattern-based framework
title_short P53 in Penile Squamous Cell Carcinoma: A Pattern-Based Immunohistochemical Framework with Molecular Correlation
title_full P53 in Penile Squamous Cell Carcinoma: A Pattern-Based Immunohistochemical Framework with Molecular Correlation
title_fullStr P53 in Penile Squamous Cell Carcinoma: A Pattern-Based Immunohistochemical Framework with Molecular Correlation
title_full_unstemmed P53 in Penile Squamous Cell Carcinoma: A Pattern-Based Immunohistochemical Framework with Molecular Correlation
title_sort P53 in Penile Squamous Cell Carcinoma: A Pattern-Based Immunohistochemical Framework with Molecular Correlation
dc.creator.none.fl_str_mv Trias, I
Saco, A
Marimon, L
del Campo, R
Manzotti, C
Ordi, O
del Pino, M
Perez, FM
Vega, N
Alos, S
Martinez, A
Rodriguez-Carunchio, L
Reig, O
Jares, P
Teixido, C
Ajami, T
Corral-Molina, JM
Algaba, F
Ribal, MJ
Ribera-Cortada, I
Rakislova, N
author Trias, I
author_facet Trias, I
Saco, A
Marimon, L
del Campo, R
Manzotti, C
Ordi, O
del Pino, M
Perez, FM
Vega, N
Alos, S
Martinez, A
Rodriguez-Carunchio, L
Reig, O
Jares, P
Teixido, C
Ajami, T
Corral-Molina, JM
Algaba, F
Ribal, MJ
Ribera-Cortada, I
Rakislova, N
author_role author
author2 Saco, A
Marimon, L
del Campo, R
Manzotti, C
Ordi, O
del Pino, M
Perez, FM
Vega, N
Alos, S
Martinez, A
Rodriguez-Carunchio, L
Reig, O
Jares, P
Teixido, C
Ajami, T
Corral-Molina, JM
Algaba, F
Ribal, MJ
Ribera-Cortada, I
Rakislova, N
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv p53 immunohistochemistry
TP53 mutations
penile squamous cell carcinoma
surrogate marker
pattern-based framework
topic p53 immunohistochemistry
TP53 mutations
penile squamous cell carcinoma
surrogate marker
pattern-based framework
description Simple Summary: Penile squamous cell carcinomas harbouring mutations of TP53 have an increased risk of lymph node metastases and an impaired prognosis, but the mutational analysis of the TP53 gene is not available in many pathology laboratories. Although p53 immunohistochemistry (IHC) has been proposed as an alternative to the molecular analysis, the current method of evaluation of p53 IHC has many inaccuracies. The aim of our study was to determine, in a series of 40 penile tumours, if a recently described pattern-based framework of p53 IHC evaluation correlates better than the classical method with the TP53 mutational status. Our results show that the new method has a very good correlation with TP53 mutations (95% sensitivity; 92% specificity), higher than that of the classical method, and can be considered as a reliable surrogate of the TP53 mutational status. This new framework can help clinicians to better define risk groups and refine treatment strategies. p53 immunohistochemistry (IHC) has been proposed as a surrogate for TP53 mutations in penile squamous cell carcinomas (PSCC). We aimed to evaluate the performance of a pattern-based evaluation of p53 IHC in PSCC. Human papilloma virus (HPV) DNA testing, p16 and p53 IHC, and whole exome sequencing were performed in a series of 40 PSCC. p53 IHC was evaluated following a pattern-based framework and conventional p53 IHC evaluation. Out of 40 PSCC, 12 (30.0%) were HPV-associated, and 28 (70.0%) were HPV-independent. The agreement between the p53 IHC pattern-based evaluation and TP53 mutational status was almost perfect (k = 0.85). The sensitivity and accuracy of the pattern-based framework for identifying TP53 mutations were 95.5% and 92.5%, respectively, which were higher than the values of conventional p53 IHC interpretation (54.5% and 70.0%, respectively), whereas the specificity was the same (88.9%). In conclusions, the pattern-based framework improves the accuracy of detecting TP53 mutations in PSCC compared to the classical p53 IHC evaluation.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=16449
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85160626389&doi=10.3390%2fcancers15102719&partnerID=40&md5=608b28fd92daa6805ccf07bb73e1a7dd
url https://iibsantpau.fundanetsuite.com/Publicaciones/ProdCientif/PublicacionFrw.aspx?id=16449
https://www.scopus.com/inward/record.uri?eid=2-s2.0-85160626389&doi=10.3390%2fcancers15102719&partnerID=40&md5=608b28fd92daa6805ccf07bb73e1a7dd
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Cancers
ISSN: 20726694
reponame:r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
instname:Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
instname_str Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
reponame_str r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
collection r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
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