Estudio de fármacos inhibidores de autofagia y epigenéticos en una línea celular de cáncer de próstata

Regulation of epigenetic and autophagy has an important role in prostate cancer. On the one hand, the impact of epigenetic modifications is engaged in proliferation, distinction and tumors survival. Panobinostat is an intense pan-deacetylases inhibitor which can reverse epigenetic changes involved i...

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Detalles Bibliográficos
Autores: Sánchez Arroyo, Carmen, González Sarmiento, Rogelio
Tipo de recurso: artículo
Fecha de publicación:2018
País:España
Institución:Universidad de Salamanca (USAL)
Repositorio:GREDOS. Repositorio Institucional de la Universidad de Salamanca
OAI Identifier:oai:gredos.usal.es:10366/136102
Acceso en línea:http://hdl.handle.net/10366/136102
Access Level:acceso abierto
Palabra clave:autofagia
Cloroquina
epigenética
Panobinostat
cáncer de próstata
Autophagy
Chloroquine
Epigenetic
Prostate cancer
Descripción
Sumario:Regulation of epigenetic and autophagy has an important role in prostate cancer. On the one hand, the impact of epigenetic modifications is engaged in proliferation, distinction and tumors survival. Panobinostat is an intense pan-deacetylases inhibitor which can reverse epigenetic changes involved in tumour development. On the other hand, autophagy in starting phases inhibits tumorigenesis endowing anti-oncogenic functions. Nonetheless, in ending phases, spreads metastasis and chemotherapy tolerance. Chloroquine is an antimalarial, it has an antineoplasic effect which  is being studying due to its autophagy inhibition. The aim of this project is to assess the cytotoxic effect of Chloroquine and Panobinostat over prostate cellular line VCAP through MTT assay, and analyse the effect of drugs, covering both; concrete and synergistic, over the cellular cycle. Because of the results obtained, it is shown that both drugs cause an antitumor effect in VCAP, being important to point out Panobinostat; it has also been shown that  powerful synergetic effect is responsible of  death of most cells tumour. To conclude, it would be a good treatment in patients who have been diagnosed with prostate cancer