Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells

[Background] Conflicting data exist on the role of pulmonary dendritic cells (DCs) and their maturation in patients with chronic obstructive pulmonary disease (COPD). Herein, we investigated whether disease severity and smoking status could affect the distribution and maturation of DCs in lung tissu...

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Autores: Arellano-Orden, Elena, Calero-Acuña, Carmen, Moreno-Mata, Nicolás, Gómez Izquierdo, Lourdes, Sánchez-López, Verónica, López-Ramírez, Cecilia, Tobar, Daniela, López-Villalobos, José Luis, Gutiérrez Rivera, César, Blanco-Orozco, Ana, López-Campos, J. L.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/180694
Acesso em linha:http://hdl.handle.net/10261/180694
Access Level:acceso abierto
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spelling Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic CellsArellano-Orden, ElenaCalero-Acuña, CarmenMoreno-Mata, NicolásGómez Izquierdo, LourdesSánchez-López, VerónicaLópez-Ramírez, CeciliaTobar, DanielaLópez-Villalobos, José LuisGutiérrez Rivera, CésarBlanco-Orozco, AnaLópez-Campos, J. L.[Background] Conflicting data exist on the role of pulmonary dendritic cells (DCs) and their maturation in patients with chronic obstructive pulmonary disease (COPD). Herein, we investigated whether disease severity and smoking status could affect the distribution and maturation of DCs in lung tissues of patients undergoing elective pneumectomy or lobectomy for suspected primary lung cancer.[Materials and Methods] A total of 75 consecutive patients were included. Spirometry testing was used to identify COPD. Lung parenchyma sections anatomically distant from the primary lesion were examined. We used flow cytometry to identify different DCs subtypes—including BDCA1-positive myeloid DCs (mDCs), BDCA3-positive mDCs, and plasmacytoid DCs (pDCs)—and determine their maturation markers (CD40, CD80, CD83, and CD86) in all participants. We also identified follicular DCs (fDCs), Langerhans DCs (LDCs), and pDCs in 42 patients by immunohistochemistry.[Results] COPD was diagnosed in 43 patients (16 current smokers and 27 former smokers), whereas the remaining 32 subjects were classified as non-COPD (11 current smokers, 13 former smokers, and 8 never smokers). The number and maturation of DCs did not differ significantly between COPD and non-COPD patients. However, the results of flow cytometry indicated that maturation markers CD40 and CD83 of BDCA1-positive mDCs were significantly decreased in smokers than in non-smokers (P = 0.023 and 0.013, respectively). Immunohistochemistry also revealed a lower number of LDCs in COPD patients than in non-COPD subjects.[Conclusions] Cigarette smoke, rather than airflow limitation, is the main determinant of impaired DCs maturation in the lung.This study was supported by a grant from the Instituto de Salud Carlos III, Ministerio de Economía y Competitividad, Fondo de Investigación Sanitaria (project PS09/00826).Peer reviewedPublic Library of ScienceInstituto de Salud Carlos IIIMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201920192016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/180694reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1371/journal.pone.0152737Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1806942026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
title Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
spellingShingle Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
Arellano-Orden, Elena
title_short Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
title_full Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
title_fullStr Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
title_full_unstemmed Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
title_sort Cigarette Smoke Decreases the Maturation of Lung Myeloid Dendritic Cells
dc.creator.none.fl_str_mv Arellano-Orden, Elena
Calero-Acuña, Carmen
Moreno-Mata, Nicolás
Gómez Izquierdo, Lourdes
Sánchez-López, Verónica
López-Ramírez, Cecilia
Tobar, Daniela
López-Villalobos, José Luis
Gutiérrez Rivera, César
Blanco-Orozco, Ana
López-Campos, J. L.
author Arellano-Orden, Elena
author_facet Arellano-Orden, Elena
Calero-Acuña, Carmen
Moreno-Mata, Nicolás
Gómez Izquierdo, Lourdes
Sánchez-López, Verónica
López-Ramírez, Cecilia
Tobar, Daniela
López-Villalobos, José Luis
Gutiérrez Rivera, César
Blanco-Orozco, Ana
López-Campos, J. L.
author_role author
author2 Calero-Acuña, Carmen
Moreno-Mata, Nicolás
Gómez Izquierdo, Lourdes
Sánchez-López, Verónica
López-Ramírez, Cecilia
Tobar, Daniela
López-Villalobos, José Luis
Gutiérrez Rivera, César
Blanco-Orozco, Ana
López-Campos, J. L.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
description [Background] Conflicting data exist on the role of pulmonary dendritic cells (DCs) and their maturation in patients with chronic obstructive pulmonary disease (COPD). Herein, we investigated whether disease severity and smoking status could affect the distribution and maturation of DCs in lung tissues of patients undergoing elective pneumectomy or lobectomy for suspected primary lung cancer.
publishDate 2016
dc.date.none.fl_str_mv 2016
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/180694
url http://hdl.handle.net/10261/180694
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1371/journal.pone.0152737
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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