Comparative genomics-driven design of virus-delivered short RNA inserts triggering robust gene silencing
[EN] RNA products and RNA virus-based technologies have the potential to transform agriculture by enabling on-demand crop trait reprogramming and effective pest and disease management (Pasin et al., 2024; Rossner € et al., 2022). In virus-induced gene silencing (VIGS), engineered RNA viruses can red...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/230584 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/230584 |
| Access Level: | acceso abierto |
| Palabra clave: | Virus-induced gene silencing (VIGS) Tobacco rattle virus (TRV) Functional genomics Nicotiana benthamiana Solanum aethiopicum Underutilized crops |
| Sumario: | [EN] RNA products and RNA virus-based technologies have the potential to transform agriculture by enabling on-demand crop trait reprogramming and effective pest and disease management (Pasin et al., 2024; Rossner € et al., 2022). In virus-induced gene silencing (VIGS), engineered RNA viruses can redirect the host RNA interference machineries to target gene silencing through the production of gene-specific small RNAs (sRNAs) (Rossner € et al., 2022). Although endogenous sRNAs and those resulting from VIGS are in the 20¿30-nt range, VIGS vectors are engineered to deliver larger inserts of 200¿400 nt with homology to a target gene, often located in less conserved regions to ensure specificity (Ahmed et al., 2020). Reducing insert sizes, may enhance the VIGS scalability and applicability to non-model species. |
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