Performance of the cobas u 701 Analyzer in Urinary Tract Infection Screening

Background: Negative urine cultures to rule out urinary tract infections (UTI) generate a considerable laboratory workload; thus, a rapid screening test is desirable. We evaluated the performance of a new automated microscopy analyzer, cobas u 701 (Roche Diagnostics International, Rotkreuz, Switzerl...

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Detalhes bibliográficos
Autores: Ortiz de la Tabla V, Gázquez G, Infante A, Martin C, Buñuel F, Gutiérrez F
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p4600
Acesso em linha:https://fisabio.portalinvestigacion.com/publicaciones/4600
Access Level:acceso abierto
Palavra-chave:Automated microscopy analyzer
cobas u 701
Screening urine samples
Urinary tract infection
Urine culture
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spelling Performance of the cobas u 701 Analyzer in Urinary Tract Infection ScreeningOrtiz de la Tabla VGázquez GInfante AMartin CBuñuel FGutiérrez FAutomated microscopy analyzercobas u 701Screening urine samplesUrinary tract infectionUrine cultureBackground: Negative urine cultures to rule out urinary tract infections (UTI) generate a considerable laboratory workload; thus, a rapid screening test is desirable. We evaluated the performance of a new automated microscopy analyzer, cobas u 701 (Roche Diagnostics International, Rotkreuz, Switzerland) for the screening of UTI, and developed a rule-out strategy to reduce the number of samples requiring culture. We also assessed squamous epithelial cell (SEC) count as a predictor of culture contamination. Methods: In total, 1,604 urine samples from outpatients were analyzed with cobas u 701 and culture. Bacterial (BAC) and white blood cell (WBC) counts were used for sample interpretation. To determine a useful cut-off point to predict negative cultures, we selected the highest sensitivity and specificity values obtained from ROC curves. Diagnostic accuracy by age and gender was evaluated. Results: Urine culture showed growth of >= 10(4) colony forming units (CFU)/mL in 256 samples (16.0%). The highest sensitivity (91.8%) and specificity (68.4%) were obtained for cut-off points of 119 BAC/mu L and 22 WBC/mu L. The combination of BAC and WBC improved the performance of the rule-out strategy with a low rate of false-negative results (1.5%) and a high negative predictive value (NPV, 97.3%). Fifty-seven percent of the samples would not have required culture. SEC count was a poor predictor of culture contamination. Conclusions: cobas u 701 can substantially reduce the number of urine samples requiring culture, with a low false-negative rate and a high NPV.KOREAN SOC LABORATORY MEDICINE2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/4600Annals of Laboratory MedicineISSN: 22343806ISSNe: 22343814reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p46002026-06-11T12:45:17Z
dc.title.none.fl_str_mv Performance of the cobas u 701 Analyzer in Urinary Tract Infection Screening
title Performance of the cobas u 701 Analyzer in Urinary Tract Infection Screening
spellingShingle Performance of the cobas u 701 Analyzer in Urinary Tract Infection Screening
Ortiz de la Tabla V
Automated microscopy analyzer
cobas u 701
Screening urine samples
Urinary tract infection
Urine culture
title_short Performance of the cobas u 701 Analyzer in Urinary Tract Infection Screening
title_full Performance of the cobas u 701 Analyzer in Urinary Tract Infection Screening
title_fullStr Performance of the cobas u 701 Analyzer in Urinary Tract Infection Screening
title_full_unstemmed Performance of the cobas u 701 Analyzer in Urinary Tract Infection Screening
title_sort Performance of the cobas u 701 Analyzer in Urinary Tract Infection Screening
dc.creator.none.fl_str_mv Ortiz de la Tabla V
Gázquez G
Infante A
Martin C
Buñuel F
Gutiérrez F
author Ortiz de la Tabla V
author_facet Ortiz de la Tabla V
Gázquez G
Infante A
Martin C
Buñuel F
Gutiérrez F
author_role author
author2 Gázquez G
Infante A
Martin C
Buñuel F
Gutiérrez F
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Automated microscopy analyzer
cobas u 701
Screening urine samples
Urinary tract infection
Urine culture
topic Automated microscopy analyzer
cobas u 701
Screening urine samples
Urinary tract infection
Urine culture
description Background: Negative urine cultures to rule out urinary tract infections (UTI) generate a considerable laboratory workload; thus, a rapid screening test is desirable. We evaluated the performance of a new automated microscopy analyzer, cobas u 701 (Roche Diagnostics International, Rotkreuz, Switzerland) for the screening of UTI, and developed a rule-out strategy to reduce the number of samples requiring culture. We also assessed squamous epithelial cell (SEC) count as a predictor of culture contamination. Methods: In total, 1,604 urine samples from outpatients were analyzed with cobas u 701 and culture. Bacterial (BAC) and white blood cell (WBC) counts were used for sample interpretation. To determine a useful cut-off point to predict negative cultures, we selected the highest sensitivity and specificity values obtained from ROC curves. Diagnostic accuracy by age and gender was evaluated. Results: Urine culture showed growth of >= 10(4) colony forming units (CFU)/mL in 256 samples (16.0%). The highest sensitivity (91.8%) and specificity (68.4%) were obtained for cut-off points of 119 BAC/mu L and 22 WBC/mu L. The combination of BAC and WBC improved the performance of the rule-out strategy with a low rate of false-negative results (1.5%) and a high negative predictive value (NPV, 97.3%). Fifty-seven percent of the samples would not have required culture. SEC count was a poor predictor of culture contamination. Conclusions: cobas u 701 can substantially reduce the number of urine samples requiring culture, with a low false-negative rate and a high NPV.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/4600
url https://fisabio.portalinvestigacion.com/publicaciones/4600
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv KOREAN SOC LABORATORY MEDICINE
publisher.none.fl_str_mv KOREAN SOC LABORATORY MEDICINE
dc.source.none.fl_str_mv Annals of Laboratory Medicine
ISSN: 22343806
ISSNe: 22343814
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
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