The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation

Many filamentous cyanobacteria respond to the external cue of nitrogen scarcity by the differentiation of heterocysts, cells specialized in the fixation of atmospheric nitrogen in oxic environments. Heterocysts follow a spatial pattern along the filament of two heterocysts separated by ca. 10–15 veg...

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Autores: Valladares Ruiz, Ana, Flores García, Enrique, Herrero Moreno, Antonia
Tipo de recurso: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2016
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/68197
Acceso en línea:http://hdl.handle.net/11441/68197
https://doi.org/doi: 10.1111/mmi.13268
Access Level:acceso abierto
Palabra clave:Heterocyst differentiation
Post-translational modification
Anabaena
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spelling The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylationValladares Ruiz, AnaFlores García, EnriqueHerrero Moreno, AntoniaHeterocyst differentiationPost-translational modificationAnabaenaMany filamentous cyanobacteria respond to the external cue of nitrogen scarcity by the differentiation of heterocysts, cells specialized in the fixation of atmospheric nitrogen in oxic environments. Heterocysts follow a spatial pattern along the filament of two heterocysts separated by ca. 10–15 vegetative cells performing oxygenic photosynthesis. HetR is a transcriptional regulator that directs heterocyst differentiation. In the model strain Anabaena sp. PCC 7120, the HetR protein was observed in various oligomeric forms in vivo, including a tetramer that peaked with maximal hetR expression during differentiation. Tetramers were not detected in a hetR point mutant incapable of differentiation, but were conspicuous in an over-differentiating strain lacking the PatS inhibitor. In differentiated filaments the HetR tetramer was restricted to heterocysts, being undetectable in vegetative cells. HetR co-purified with RNA polymerase from Anabaena mainly as a tetramer. In vitro, purified recombinant HetR was distributed between monomers, dimers, trimers and tetramers, and it was phosphorylated when incubated with (γ-32P)ATP. Phosphorylation and PatS hampered the accumulation of HetR tetramers and impaired HetR binding to DNA. In summary, tetrameric HetR appears to represent a functionally relevant form of HetR, whose abundance in the Anabaena filament could be negatively regulated by phosphorylation and by PatS.España, Gobierno BFU2010-17980 and BFU2013-44686-PWileyBioquímica Vegetal y Biología MolecularGobierno de España2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/11441/68197https://doi.org/doi: 10.1111/mmi.13268reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMolecular Microbiology, 99 (4), 808-819.BFU2010-17980BFU2013-44686-Phttp://onlinelibrary.wiley.com/doi/10.1111/mmi.13268/epdfinfo:eu-repo/semantics/openAccessoai:idus.us.es:11441/681972026-06-17T12:51:07Z
dc.title.none.fl_str_mv The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
title The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
spellingShingle The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
Valladares Ruiz, Ana
Heterocyst differentiation
Post-translational modification
Anabaena
title_short The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
title_full The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
title_fullStr The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
title_full_unstemmed The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
title_sort The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation
dc.creator.none.fl_str_mv Valladares Ruiz, Ana
Flores García, Enrique
Herrero Moreno, Antonia
author Valladares Ruiz, Ana
author_facet Valladares Ruiz, Ana
Flores García, Enrique
Herrero Moreno, Antonia
author_role author
author2 Flores García, Enrique
Herrero Moreno, Antonia
author2_role author
author
dc.contributor.none.fl_str_mv Bioquímica Vegetal y Biología Molecular
Gobierno de España
dc.subject.none.fl_str_mv Heterocyst differentiation
Post-translational modification
Anabaena
topic Heterocyst differentiation
Post-translational modification
Anabaena
description Many filamentous cyanobacteria respond to the external cue of nitrogen scarcity by the differentiation of heterocysts, cells specialized in the fixation of atmospheric nitrogen in oxic environments. Heterocysts follow a spatial pattern along the filament of two heterocysts separated by ca. 10–15 vegetative cells performing oxygenic photosynthesis. HetR is a transcriptional regulator that directs heterocyst differentiation. In the model strain Anabaena sp. PCC 7120, the HetR protein was observed in various oligomeric forms in vivo, including a tetramer that peaked with maximal hetR expression during differentiation. Tetramers were not detected in a hetR point mutant incapable of differentiation, but were conspicuous in an over-differentiating strain lacking the PatS inhibitor. In differentiated filaments the HetR tetramer was restricted to heterocysts, being undetectable in vegetative cells. HetR co-purified with RNA polymerase from Anabaena mainly as a tetramer. In vitro, purified recombinant HetR was distributed between monomers, dimers, trimers and tetramers, and it was phosphorylated when incubated with (γ-32P)ATP. Phosphorylation and PatS hampered the accumulation of HetR tetramers and impaired HetR binding to DNA. In summary, tetrameric HetR appears to represent a functionally relevant form of HetR, whose abundance in the Anabaena filament could be negatively regulated by phosphorylation and by PatS.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11441/68197
https://doi.org/doi: 10.1111/mmi.13268
url http://hdl.handle.net/11441/68197
https://doi.org/doi: 10.1111/mmi.13268
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Molecular Microbiology, 99 (4), 808-819.
BFU2010-17980
BFU2013-44686-P
http://onlinelibrary.wiley.com/doi/10.1111/mmi.13268/epdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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